Scaffolds Loaded with Dialdehyde Chitosan and Collagen—Their Physico-Chemical Properties and Biological Assessment

Abstract

In this work, dialdehyde chitosan (DAC) and collagen (Coll) scaffolds have been prepared and their physico-chemical properties have been evaluated. Their structural properties were studied by Fourier Transform Infrared Spectroscopy with Attenuated Internal Reflection (FTIR–ATR) accompanied by evaluation of thermal stability, porosity, density, moisture content and microstructure by Scanning Electron Microscopy—SEM. Additionally, cutaneous assessment using human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF) and melanoma cells (A375 and G-361) was performed. Based on thermal studies, two regions in DTG curves could be distinguished in each type of scaffold, what can be assigned to the elimination of water and the polymeric structure degradation of the materials components. The type of scaffold had no major effect on the porosity of the materials, but the water content of the materials decreased with increasing dialdehyde chitosan content in subjected matrices. Briefly, a drop in proliferation was noticed for scaffolds containing 20DAC/80Coll compared to matrices with collagen alone. Furthermore, increased content of DAC (50DAC/50Coll) either significantly induced the proliferation rate or maintains its ratio compared to the control matrix. This delivery is a promising technique for additional explorations targeting therapies in regenerative dermatology. The using of dialdehyde chitosan as one of the main scaffolds components is the novelty in terms of bioengineering.

Hyaluronic Acid/Ellagic Acid as Materials for Potential Medical Application

“…We assessed the impacts of solvents of ellagic acid (80HA/20EA, 50HA/50EA, and 20HA/80EA) on the viability of cutaneous cells (human dermal fibroblasts and epidermal keratinocytes) compared with human melanoma models (A375 and G361) ( Figure 3 ). The selection of cell lines was in accordance with previous reports [ 25 , 26 , 27 ]. The obtained results demonstrated the significant decrease in the proliferation ratio versus the control cells, in both healthy and tumor cells.…”

Application of Collagen-Based Hydrogel in Skin Wound Healing

“…The material’s byproducts and unreacted chemicals cannot be removed after repeated washing [ 52 ]. Reactants such as 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS), genipin, and dialdehyde starch (DAS), which are significantly less toxic to cells, are substitutes for glutaraldehyde [ 53 , 54 , 55 ]. EDC-NHS is a “zero-length” crosslinking agent that can facilitate the formation of amide bonds by linking the carboxyl group of glutamic or aspartic acid collagen to the amino group.…”

Composite Matrixes for Use in Traumatology and Regenerative Medicine

“…Ряд авторов также проводил исследования хитозан-коллагеновых каркасов ex vivo и in vivo, в ходе которых была выявлена высокая степень неоваскуляризации формирующихся на каркасе тканей, а также ускорение эпителизации раны [1,4,5]. Помимо повышенной эпителизации, в моделях ран было также продемонстрировано увеличение скорости пролиферации и повышение клеточной адгезии кератиноцитов in vitro [2,7,8].…”