Scaffolds for Tissue Engineering and 3D Cell Culture

Carletti, Eleonora; Motta, Antonella; Migliaresi, Claudio

doi:10.1007/978-1-60761-984-0_2

Cited by 339 publications

(277 citation statements)

References 91 publications

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“…The scaffold functions as the in vivo ECM by providing structural and functional microenvironment (i.e., ''niche'') for cell growth, migration, and differentiation. [9][10][11][12] Although the composition of ECM environment is unique to each tissue, the major components of ECMs are collagens, fibronectin, laminin, and various types of glycoaminoglycans and proteoglycans. 13 ECM, together with growth factors, cytokines, and interactions with other cell types, renders the biological and physical cues that dictate MSC function and overall fate.…”

Section: Introductionmentioning

confidence: 99%

Stromal-Cell-Derived Extracellular Matrix Promotes the Proliferation and Retains the Osteogenic Differentiation Capacity of Mesenchymal Stem Cells on Three-Dimensional Scaffolds

Antebi

Zhang

Wang

et al. 2015

Tissue Engineering Part C: Methods

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To date, expansion of bone-marrow-derived mesenchymal stem cells (MSCs) is typically carried out on twodimensional (2D) tissue culture plastic. Since this 2D substratum is very different from the physiological situation, MSCs gradually lose their unique multipotent properties during expansion. Recently, the role of the extracellular matrix (ECM) microenvironment (''niche'') in facilitating and regulating stem cell behavior in vivo has been elucidated. As a result, investigators have shifted their efforts toward developing threedimensional (3D) scaffolds capable of functioning like the native tissue ECM. In this study, we demonstrated that stromal-cell-derived ECM, formed within a collagen/hydroxyapatite (Col/HA) scaffold to mimic the bone marrow ''niche,'' promoted MSC proliferation and preserved their differentiation capacity. The ECM was synthesized by MSCs to reconstitute the tissue-specific 3D microenvironment in vitro. Following deposition of the ECM inside Col/HA scaffold, the construct was decellularized and reseeded with MSCs to study their behavior. The data showed that MSCs cultured on the ECM-Col/HA scaffolds grew significantly faster than the cells from the same batch cultured on the regular Col/HA scaffolds. In addition, MSCs cultured on the ECMCol/HA scaffolds retained their ''stemness'' and osteogenic differentiation capacity better than MSCs cultured on regular Col/HA scaffolds. When ECM-Col/HA scaffolds were implanted into immunocompromised mice, with or without loading MSCs, it was found that those scaffolds formed less bone as compared with regular Col/ HA scaffolds (i.e., without ECM), in both cases of with or without loading MSCs. The in vivo study further confirmed that the ECM-Col/HA scaffold was a suitable mimic of the bone marrow ''niche.'' This novel 3D stromal-cell-derived ECM system has the potential to be developed into a biomedical platform for regenerative medicine applications.

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Section: Introductionmentioning

confidence: 99%

Stromal-Cell-Derived Extracellular Matrix Promotes the Proliferation and Retains the Osteogenic Differentiation Capacity of Mesenchymal Stem Cells on Three-Dimensional Scaffolds

Antebi

Zhang

Wang

et al. 2015

Tissue Engineering Part C: Methods

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“…It was confirmed that design of degradation scaffold plays an important role due to appropriate environment for the cell to adhere, proliferate, differentiate and generate an extracellular matrix for tissue regeneration [15].…”

Section: Figure 5 Fibroblast Proliferation On Scaffoldsmentioning

confidence: 87%

Degradation profile and fibroblast proliferation on synthetic coral scaffold for bone regeneration

Mahanani¹,

Herningtyas²,

Bachtiar³

et al. 2016

AIP Conference Proceedings

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COLLECTIONS This paper was selected as Featured ARTICLES YOU MAY BE INTERESTED INChitosan-gelatine membrane construct with different cinnamaldehyde concentration as drug delivery system in oral cavity AIP Conference Proceedings 1755, 160006 (2016) 160007 (2016) At the request of all authors of the paper, and with the agreement of the Proceedings Editor, an updated version of this article was published on 27 November 2018. The original version supplied to AIP Publishing included an error in the name of an author, name was listed as Andika Dewi Ana rather than Ika Dewi Ana. This has been corrected in the updated and republished version. Abstract. For bone regeneration, one of the important key factors is the ability of the scaffold to be able to be degraded gradually at the implant side and the ability to provide a microenvironment for cells. No information available yet regarding our new synthetic coral scaffold. The purpose of this study was to observe the degradation profile and the human fibroblast gingiva proliferation on the synthetic coral scaffolds prepared from gelatin and calcium carbonate (CaCO3) in a various concentration of weight% wherein gelatin only (G) was used as a control. Scaffolds were incubated in non-phenol red DMEM for 1, 3, 6, 24, 48, and 72 h, then the supernatant was analyzed by spectrophotometer in 280 nm wavelength. After 72 hours, the degradation rate of the scaffold was accelerated using 1N HCl.The proliferation of human fibroblast gingiva was observed at 24, 48 and 72 hours after seeding 2.10 4 cells/well. A plate of 96 well and DMEM were used in this study. The degradation percentage of the scaffolds within 24 hours was considered small, ranging between 4.23 -5.15%. Meanwhile, the degradation between 24 to 72 hours incubation was confirmed at 38. 35, 62.16, 52.86, 47.86, 48.42, and 53.17% for G, 7/3, 6/4, 5/5, 4/6 and 3/7 respectively. In the accelerated condition, the fastest degradation was found in G.Fibroblast proliferation increased following the incubation time except in control group and 3:7, but no significantly different between the various concentration. The fastest degradation rate was found in the scaffold without CaCO3 addition, confirming that CaCO3 in certain concentration prolonged the half-life of the scaffold. The proliferation of fibroblast is increased for all incubation time. Degradation Profile and Fibroblast Proliferation on Synthetic Coral Scaffold for Bone Regeneration

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“…Les procédés d'ingénierie tissulaire font appel, dans la plupart des cas, à des matériaux poreux, structure-support pour la croissance (scaffolds). La constitution de ces scaffolds est essentielle pour assurer la multiplication et la différenciation des cellules [5]. Le scaffold pourra aussi contenir des vecteurs d'expression de gènes ou des facteurs de croissance cellulaire ou encore d'autres molécules susceptibles de guider les cellules et d'induire leur différencia-tion.…”

Section: E Fattalunclassified

Matériaux pour la médecine de demain

Fattal

2017

Med Sci (Paris)

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Éditorial> Depuis l'Antiquité, l'homme a tenté de réparer le corps humain ou de remplacer un tissu lésé par un autre tissu ou un substitut tout aussi viable et fonctionnel. Les premières tentatives d'implantation de dents remontent ainsi au temps de l'Égypte ancienne et des cultures précolombiennes. Elles étaient sculptées dans l'ivoire ou dans le bois et retenues entre elles par des fils d'or. Aujourd'hui, si on se réfère à la conférence (ou assistance) fonctionnelle ». La première génération de biomatériaux d'origine naturelle a progressivement été remplacée par des implants fabriqués à partir de polymères synthétiques, de céramiques, de métaux et d'alliages métalliques, qui sont constitués de composés qui vont entrer en contact avec les fluides biologiques. Il faudra qu'ils remplissent leur fonction tout en étant biocompatibles, c'est-à-dire sans activer la cascade du complément ou modifier la coagulation sanguine ou encore sans induire des réactions inflammatoires délétères pour le corps humain. Il faudra veiller à ce que les matériaux ne soient pas altérés, c'est-à-dire que les produits issus de leur dégradation ou de leur vieillissement n'entraînent pas des effets indésirables pour l'organisme. C'est dans le domaine des polymères que des avancées considérables ont pu être réalisées. En effet, une meilleure caractérisation de ces polymères, une amélioration de leurs techniques de synthèse et enfin leur transformation en nanotechnologies sont tout autant d'éléments qui ont permis des avancées considérables en implantologie. L'utilisation des biomatériaux ne s'est pas limitée aux dispositifs médicaux. Dans le domaine du médicament, la première utilisation de ces matériaux a été destinée à développer des systèmes pour la libération contrôlée de substances actives. De nombreux travaux ont eu pour but de démontrer la possibilité de contrôler dans le temps la libération des principes actifs, et ce pour des durées pouvant varier de plusieurs semaines à plusieurs années, réduisant ainsi les fréquences d'administration. C'est ainsi que, par exemple, sont nés des implants non biodé-1 Conférence organisée par la Société Européenne pour les Biomatériaux, faisant suite à une première conférence de 1986, ayant permis d'arriver à un consensus sur la définition et les attributs des biomatériaux et de la biocompatibilité.gradables pour la voie sous-cutanée comme le Nexplanon®, utilisé en substitut de la contraception orale, ou encore des implants biodégradables pour la libération contrôlée de peptides comme le Décapeptyl®, utilisé dans le traitement du cancer de la prostate, ou enfin pour la voie intraoculaire, un implant à base de dexaméthasone, l'Ozurdex®. Outre le confort que procure une telle approche pour le patient, l'utilisation de systèmes à libération contrôlée permet d'administrer localement des principes actifs dans des sanctuaires de l'organisme où la répétition des administrations est complexe et/ou l'utilisation de voies d'administration plus classiques est impossible. L'utilisation de biomatériaux a aussi ...

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Scaffolds for Tissue Engineering and 3D Cell Culture

Cited by 339 publications

References 91 publications

Stromal-Cell-Derived Extracellular Matrix Promotes the Proliferation and Retains the Osteogenic Differentiation Capacity of Mesenchymal Stem Cells on Three-Dimensional Scaffolds

Stromal-Cell-Derived Extracellular Matrix Promotes the Proliferation and Retains the Osteogenic Differentiation Capacity of Mesenchymal Stem Cells on Three-Dimensional Scaffolds

Degradation profile and fibroblast proliferation on synthetic coral scaffold for bone regeneration

Matériaux pour la médecine de demain

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