Scaffolds for skeletal muscle tissue engineering

Bartolacci, Joseph; Dziki, Jenna L.; Badylak, Stephen F.

doi:10.1016/b978-0-08-102563-5.00012-5

Cited by 4 publications

(3 citation statements)

References 103 publications

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“…Interestingly, some of them have shown to promote a M2balanced response (Sicari et al, 2014;Boersema et al, 2016;Wang et al, 2019). These scaffolds are intended to give mechanical and biochemical support to the different types of cells involved in the regeneration process, such as SCs, and they need to be biodegradable to give space for the formation of new myofibers (Wolf et al, 2015;Bartolacci et al, 2019). One specific type of scaffolds are injectable hydrogels, which are made up of polymeric biomaterials that form 3D networks with high water content and permeability and that can be applied in a minimally invasive way.…”

Section: Introductionmentioning

confidence: 99%

Elastin-Like Recombinamer Hydrogels for Improved Skeletal Muscle Healing Through Modulation of Macrophage Polarization

Ibáñez‐Fonseca

Maniega

Blanco

et al. 2020

Front. Bioeng. Biotechnol.

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Large skeletal muscle injuries, such as a volumetric muscle loss (VML), often result in an incomplete regeneration due to the formation of a non-contractile fibrotic scar tissue. This is, in part, due to the outbreak of an inflammatory response, which is not resolved over time, meaning that type-1 macrophages (M1, pro-inflammatory) involved in the initial stages of the process are not replaced by pro-regenerative type-2 macrophages (M2). Therefore, biomaterials that promote the shift from M1 to M2 are needed to achieve optimal regeneration in VML injuries. In this work, we used elastin-like recombinamers (ELRs) as biomaterials for the formation of non-(physical) and covalently (chemical) crosslinked bioactive and biodegradable hydrogels to fill the VML created in the tibialis anterior (TA) muscles of rats. These hydrogels promoted a higher infiltration of M2 within the site of injury in comparison to the non-treated control after 2 weeks (p<0.0001), indicating that the inflammatory response resolves faster in the presence of both types of ELR-based hydrogels. Moreover, there were not significant differences in the amount of collagen deposition between the samples treated with the chemical ELR hydrogel at 2 and 5 weeks, and this same result was found upon comparison of these samples with healthy tissue after 5 weeks, which implies that this treatment prevents fibrosis. The macrophage modulation also translated into the formation of myofibers that were morphologically more similar to those present in healthy muscle. Altogether, these results highlight that ELR hydrogels provide a friendly niche for infiltrating cells that biodegrades over time, leaving space to new muscle tissue. In addition, they orchestrate the shift of macrophage population toward M2, which resulted in the prevention of fibrosis in the case of the chemical hydrogel treatment and in a more healthy-like myofiber phenotype for both types of hydrogels. Further studies should focus in the assessment of the regeneration of skeletal muscle in larger animal models, where a more critical defect can be created and additional methods can be used to evaluate the functional recovery of skeletal muscle.

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Section: Introductionmentioning

confidence: 99%

Elastin-Like Recombinamer Hydrogels for Improved Skeletal Muscle Healing Through Modulation of Macrophage Polarization

Ibáñez‐Fonseca

Maniega

Blanco

et al. 2020

Front. Bioeng. Biotechnol.

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“…These constructs can be used to replace damaged muscle tissues, act as in vitro models for understanding the growth and development mechanisms of the muscular system, and for testing different drugs for treatment of muscular diseases and injuries [6,7]. Mimicking highly packed and organized cellular organization of the native muscle tissues, using natural or synthetic scaffolds and microscale technologies, is crucial for successful engineering of skeletal muscle tissues [8,9].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

et al. 2019

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Skeletal muscle tissue engineering aims to fabricate tissue constructs to replace or restore diseased or injured skeletal muscle tissues in the body. Several biomaterials and microscale technologies have been used in muscle tissue engineering. However, it is still challenging to mimic the function and structure of the native muscle tissues. Three-dimensional (3D) bioprinting is a powerful tool to mimic the hierarchical structure of native tissues. Here, 3D bioprinting was used to fabricate tissue constructs using gelatin methacryloyl (GelMA)-alginate bioinks. Mechanical and rheological properties of GelMA-alginate hydrogels were characterized. C2C12 myoblasts at the density 8 × 106 cells/mL were used as the cell model. The effects of alginate concentration (0, 6, and 8% (w/v)) and crosslinking mechanism (UV crosslinking or ionic crosslinking with UV crosslinking) on printability, cell viability, proliferation, and differentiation of bioinks were studied. The results showed that 10% (w/v) GelMA-8% (w/v) alginate crosslinked using UV light and 0.1 M CaCl2 provided the optimum niche to induce muscle tissue formation compared to other hydrogel compositions. Furthermore, metabolic activity of cells in GelMA bioinks was improved by addition of oxygen-generating particles to the bioinks. It is hoped that such bioprinted muscle tissues may find wide applications in drug screening and tissue regeneration.

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“…There are a large number of different materials available, and the benefits and disadvantages of the individual ones have already been covered by several extensive reviews and shall not be covered in detail here [ 33 , 34 , 35 , 36 ]. In brief terms, the choice of scaffold depends primarily on the desired outcome, fabrication method and cellular inclusion.…”

Section: Scaffold Composition Topography and Fabricationmentioning

confidence: 99%

Next Stage Approach to Tissue Engineering Skeletal Muscle

et al. 2020

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Large-scale muscle injury in humans initiates a complex regeneration process, as not only the muscular, but also the vascular and neuro-muscular compartments have to be repaired. Conventional therapeutic strategies often fall short of reaching the desired functional outcome, due to the inherent complexity of natural skeletal muscle. Tissue engineering offers a promising alternative treatment strategy, aiming to achieve an engineered tissue close to natural tissue composition and function, able to induce long-term, functional regeneration after in vivo implantation. This review aims to summarize the latest approaches of tissue engineering skeletal muscle, with specific attention toward fabrication, neuro-angiogenesis, multicellularity and the biochemical cues that adjuvate the regeneration process.

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Scaffolds for skeletal muscle tissue engineering

Cited by 4 publications

References 103 publications

Elastin-Like Recombinamer Hydrogels for Improved Skeletal Muscle Healing Through Modulation of Macrophage Polarization

Elastin-Like Recombinamer Hydrogels for Improved Skeletal Muscle Healing Through Modulation of Macrophage Polarization

Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

Next Stage Approach to Tissue Engineering Skeletal Muscle

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