Scaffolds for Articular Cartilage Repair

Olson, Ashley; Graver, Adam; Grande, Daniel

doi:10.1615/jlongtermeffmedimplants.2013006556

Cited by 13 publications

(9 citation statements)

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“…[15][16][17][18] Superficial cartilage defects have mainly been treated with hyaluronic acid (HA), while chondral and osteochondral lesions have been medicated by means of HA and autologous cartilage transplantation (ACT) since 1994. [19][20][21][22][23] In the latter case, the use of matrixassisted autologous chondrocyte transplantation has become increasingly attractive since 1998, because articular cartilage defects of varying degrees can be replenished more easily and in a more targeted manner.…”

Section: Introductionmentioning

confidence: 99%

Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds

Pasold

Zander

Heskamp

et al. 2015

IJN

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Purpose In the present study, silica nanoparticles (sNP) coupled with insulin-like growth factor 1 (IGF-1) were loaded on a collagen-based scaffold intended for cartilage repair, and the influence on the viability, proliferation, and differentiation potential of human primary articular chondrocytes was examined. Methods Human chondrocytes were isolated from the hyaline cartilage of patients (n=4, female, mean age: 73±5.1 years) undergoing primary total knee joint replacement. Cells were dedifferentiated and then cultivated on a bioresorbable collagen matrix supplemented with fluorescent sNP coupled with IGF-1 (sNP–IGF-1). After 3, 7, and 14 days of cultivation, cell viability and integrity into the collagen scaffold as well as metabolic cell activity and synthesis rate of matrix proteins (collagen type I and II) were analyzed. Results The number of vital cells increased over 14 days of cultivation, and the cells were able to infiltrate the collagen matrix (up to 120 μm by day 7). Chondrocytes cultured on the collagen scaffold supplemented with sNP–IGF-1 showed an increase in metabolic activity (5.98-fold), and reduced collagen type I (1.58-fold), but significantly increased collagen type II expression levels (1.53-fold; P =0.02) after 7 days of cultivation compared to 3 days. In contrast, chondrocytes grown in a monolayer on plastic supplemented with sNP-IGF-1 had significantly lower metabolic activity (1.32-fold; P =0.007), a consistent amount of collagen type I, and significantly reduced collagen type II protein expression (1.86-fold; P =0.001) after 7 days compared to 3 days. Conclusion Collagen-based scaffolds enriched with growth factors, such as IGF-1 coupled to nanoparticles, represent an improved therapeutic intervention for the targeted and controlled treatment of articular cartilage lesions.

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Section: Introductionmentioning

confidence: 99%

Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds

Pasold

Zander

Heskamp

et al. 2015

IJN

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“…In addition, scaffolds can be used to achieve drug delivery with high-loading efficiency at specific sites. Biologic scaffolds employed to date as natural polymers include alginate, collagen, fibrin, albumin, hyaluronan, platelet-rich plasma, and gelatin7.…”

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confidence: 99%

A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

Mazaki

Sugiyama

Yamane

et al. 2014

Sci Rep

110

106

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Osteochondral injuries remain difficult to repair. We developed a novel photo-cross-linkable furfurylamine-conjugated gelatin (gelatin-FA). Gelatin-FA was rapidly cross-linked by visible light with Rose Bengal, a light sensitizer, and was kept gelled for 3 weeks submerged in saline at 37°C. When bone marrow-derived stromal cells (BMSCs) were suspended in gelatin-FA with 0.05% Rose Bengal, approximately 87% of the cells were viable in the hydrogel at 24 h after photo-cross-linking, and the chondrogenic differentiation of BMSCs was maintained for up to 3 weeks. BMP4 fusion protein with a collagen binding domain (CBD) was retained in the hydrogels at higher levels than unmodified BMP4. Gelatin-FA was subsequently employed as a scaffold for BMSCs and CBD-BMP4 in a rabbit osteochondral defect model. In both cases, the defect was repaired with articular cartilage-like tissue and regenerated subchondral bone. This novel, photo-cross-linkable gelatin appears to be a promising scaffold for the treatment of osteochondral injury.

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“…Tanto a formação quanto a funcionalidade do tecido resultante estarão, dessa forma, diretamente relacionadas à mecanoquímica da malha molecular onde as células formadoras do tecido em questão se encontram [36,37] . A adesão celular a um biomaterial está relacionada a dois fenômenos diferentes: (1) [38] . Os sítios de adesão entre as células e o substrato são chamados de contatos focais ou placas de adesão, sendo a distância de união entre ambos em torno de 10-20 nm.…”

Section: Dinâmica Celular: Adesão Proliferação E Migração Celularunclassified

“…A cartilagem articular é conhecida por ter uma pobre capacidade intrínseca para o reparo, devido em grande parte à sua avascularidade e ao acesso limitado às células reparadoras. As células deste tecido apresentam baixo índice mitótico, o que contribui para a sua limitada capacidade de regeneração [1] .…”

Section: Introductionunclassified

Hidrogéis a base de ácido hialurônico e quitosana para engenharia de tecido cartilaginoso

Nascimento

Lombello

2016

Polímeros

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AesumoA Engenharia de Tecidos envolve o desenvolvimento de novos materiais ou dispositivos capazes de interações específicas com os tecidos biológicos, buscando a utilização de materiais biocompatíveis que devem servir como arcabouço para o crescimento de células in vitro, organizando e desenvolvendo o tecido que posteriormente será implantado no paciente. Uma variedade de arcabouços como hidrogéis poliméricos, sintéticos e naturais, têm sido investigados para a expansão de condrócitos in vitro, visando o reparo da cartilagem lesionada. Um hidrogel de interesse particular na regeneração de cartilagem é o ácido hialurónico (AH). Trata-se de um biopolímero atraente para a fabricação de arcabouços artificiais para Engenharia de Tecidos por ser biocompatível e biodegradável. A biocompatibilidade do AH deve-se ao fato de estar presente na matriz extracelular nativa, deste modo, cria-se um ambiente propício que facilita a adesão, proliferação e diferenciação celular, além da existência de sinalização celular específica, o que contribui para a regeneração do tecido. O uso de hidrogel composto de ácido hialurónico e quitosana (QUI) também tem sido investigado em aplicações de Engenharia de Tecidos de cartilagem, com resultados promissores. Baseando-se nestas informações, o objetivo este trabalho foi investigar as alternativas disponíveis para regeneração tecidual da cartilagem e conhecer mais detalhadamente as relações entre células e biomateriais. Palavras-chave: ácido hialurônico, biocompatibilidade, engenharia de tecidos, quitosana, cultura de células. AbstractTissue Engineering involves the development of new materials or devices capable of specific interactions with biological tissues, searching the use of biocompatible materials as scaffolds for cell growth in vitro, organizing and developing tissue that is subsequently implanted into the patient. A variety of scaffolds such as polymeric hydrogels, natural and synthetic, have been investigated for the expansion of chondrocytes in vitro in order to repair the damaged cartilage. A hydrogel of particular interest in cartilage regeneration is hyaluronic acid (HA). HA are attractive biopolymers for manufacturing artificial scaffolds for Tissue Engineering, it is biocompatible and biodegradable. The biocompatibility of HA is due to the fact that it is present in native extracellular matrix, thus creates an environment, which facilitates the adhesion, proliferation and differentiation, in addition to the existence of specific cell signaling, which contributes to tissue regeneration. The use of hydrogel composed of hyaluronic acid and chitosan (CHI) has also been investigated for applications in Tissue Engineering of soft tissues, like cartilage, with promising results. Based on this information, this study aims to investigate the alternatives available for cartilage tissue regeneration and meet more detail the relationships between cells and biomaterials.

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Scaffolds for Articular Cartilage Repair

Cited by 13 publications

References 0 publications

Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds

Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds

A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

Hidrogéis a base de ácido hialurônico e quitosana para engenharia de tecido cartilaginoso

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