Scaffolds based on hyaluronan crosslinked with a polyaminoacid: Novel candidates for tissue engineering application

Pitarresi, Giovanna; Palumbo, Fabio Salvatore; Cavallaro, Gennara; Farè, Silvia; Giammona, Gaetano

doi:10.1002/jbm.a.31825

Cited by 10 publications

(8 citation statements)

References 33 publications

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“…These preparation approaches always involve toxic reagents and limit their biomedical applications. Several semi‐synthetic hydrogels were prepared by combined natural polymer with PAsp derivatives, especially polyaspartyl hydrazide (PAHy) . Zhang et al prepared an injectable hyaluronic acid (HA)‐PAHy hydrogel for protein delivery.…”

Section: Introductionmentioning

confidence: 99%

“…Several semi-synthetic hydrogels were prepared by combined natural polymer with PAsp derivatives, especially polyaspartyl hydrazide (PAHy). 18,40,41 Zhang et al 18 prepared an injectable hyaluronic acid (HA)-PAHy hydrogel for protein delivery. The HA derivative carrying dialdehyde was shown to react fast (30 s) with PAHy under mild conditions, and produced a hydrazone conjugated hydrogel without addition of crosslinker or catalyst.…”

Section: Introductionmentioning

confidence: 99%

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An injectable and biodegradable hydrogel based on poly(α,β‐aspartic acid) derivatives for localized drug delivery

Wang

et al. 2013

J Biomedical Materials Res

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An injectable hydrogel via hydrazone cross-linking was prepared under mild conditions without addition of cross-linker or catalyst. Hydrazine and aldehyde modified poly(aspartic acid)s were used as two gel precursors. Both of them are water-soluble and biodegradable polymers with a protein-like structure, and obtained by aminolysis reaction of polysuccinimide. The latter can be prepared by thermal polycondensation of aspartic acid. Hydrogels were prepared in PBS solution and characterized by different methods including gel content and swelling, Fourier transformed-infrared spectroscopy, and in vitro degradation experiment. A scanning electron microscope viewed the interior morphology of the obtained hydrogels, which showed porous three-dimensional structures. Different porous sizes were present, which could be well controlled by the degree of aldehyde substitution in precursor poly(aspartic acid) derivatives. The doxorubicin-loaded hydrogels were prepared and showed a pH-sensitive release profile. The release rate can be accelerated by decreasing the environmental pH from a physiological to a weak acidic condition. Moreover, the cell adhesion and growth behaviors on the hydrogel were studied and the polymeric hydrogel showed good biocompatibility.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An injectable and biodegradable hydrogel based on poly(α,β‐aspartic acid) derivatives for localized drug delivery

Wang

et al. 2013

J Biomedical Materials Res

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“…Native HA has an extremely high molecular weight, up to millions of Daltons. To reduce hydrolysis by hyaluronidase, HA gels are usually formed by crosslinking, which often employs the carboxyl groups of HA that can react covalently with different crosslinkers [85,86]. Crosslinking can reduce the biocompatibility of HA.…”

Section: Controlled Delivery Of Angiogenic Factorsmentioning

confidence: 99%

Therapeutic Angiogenesis: Controlled Delivery of Angiogenic Factors

Chu

Wang

2012

Therapeutic Delivery

125

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Therapeutic angiogenesis aims at treating ischemic diseases by generating new blood vessels from existing vasculature. It relies on delivery of exogenous factors to stimulate neovasculature formation. Current strategies using genes, proteins and cells have demonstrated efficacy in animal models. However, clinical translation of any of the three approaches has proved to be challenging for various reasons. Administration of angiogenic factors is generally considered safe, according to accumulated trials, and offers off-the-shelf availability. However, many hurdles must be overcome before therapeutic angiogenesis can become a true human therapy. This article will highlight protein-based therapeutic angiogenesis, concisely review recent progress and examine critical challenges. We will discuss growth factors that have been widely utilized in promoting angiogenesis and compare their targets and functions. Lastly, since bolus injection of free proteins usually result in poor outcomes, we will focus on controlled release of proteins.Blood vessels that carry oxygen, nutrients, cells and signals are critical in both developmental and adult physiology. Without sufficient blood supply, tissues and organs cannot maintain regular activities. On the other hand, induction of neovasculature provides a potential strategy to treat many ischemic illnesses, especially cardiovascular diseases (CVDs) including coronary and peripheral arterial diseases. The morbidity, mortality and cost of CVDs are highlighted by the latest statistics from the American Heart Association [1]: an estimated 82,600,000 American adults (≥20 years old) have one or more types of CVDs; CVDs caused 813,804 of all 2,243,712 deaths (33.6%) or one of every 2.9 deaths in 2007, and coronary heart disease caused approximately one of every six deaths. The direct and indirect cost of CVD was estimated to be US$286 billion in 2007. The amount is higher than the $228 billion spent on cancer and benign neoplasms. As a potential therapy to revascularize ischemic tissues, including ischemic heart, therapeutic angiogenesis has drawn much attention in the last 20 years.Neovasculature can be obtained by three approaches based on different mechanisms: promoting expression of angiogenic genes, supplying potent angiogenic factors and delivering progenitor or stem cells. No matter which approach is used, angiogenic activity has to be precisely controlled in order to achieve stable vascularization. For cell delivery, the

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“…In our previous patent, a hybrid hydrogel obtained by a chemical cross-linking between HA and a synthetic polyaminoacid such as α,β-poly(aspartylhydrazide) (PAHy) has been reported and now it is developed as a porous scaffold for cartilage regeneration. 10,11 More recently our research group has prepared a new hybrid scaffold based on HA chemically cross-linked with the α,β-poly(N-2-hydroxyethyl)(2-aminoethylcarbamate)-D,Laspartamide (PHEA-EDA). 12,13 Our idea was to exploit the presence of this polyaminoacid derivative (PHEA-EDA) to improve cell attachment, mechanical performances and hydrolytic resistance if compared to hyaluronic acid alone.…”

Section: Introductionmentioning

confidence: 99%

Mechanical characterization of polysaccharide/polyaminoacid hydrogels as potential scaffolds for tissue regeneration

et al. 2011

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The mechanical properties of hydrogel scaffolds based on hyaluronic acid (HA) that were chemically crosslinked with α,β-poly(N-2-hydroxyethyl) (2-aminoethylcarbamate)-D,L-aspartamide (PHEA-EDA) were investigated. Variation of these properties as a function of three different PHEA-EDA amounts used to crosslink HA has been related to the reaction efficiency evaluated using a colorimetric assay. Moreover, the amount of unreacted amino groups that was still present in the hydrogels was related to the attachment behavior of human dermal fibroblasts to the hydrogel surface. The mechanical data and biological results suggest the suitability of the investigated hydrogels as scaffolds for the regeneration of soft tissues such as skin. © 2011 The Polymer Society of Korea and Springer Netherlands

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Scaffolds based on hyaluronan crosslinked with a polyaminoacid: Novel candidates for tissue engineering application

Cited by 10 publications

References 33 publications

An injectable and biodegradable hydrogel based on poly(α,β‐aspartic acid) derivatives for localized drug delivery

An injectable and biodegradable hydrogel based on poly(α,β‐aspartic acid) derivatives for localized drug delivery

Therapeutic Angiogenesis: Controlled Delivery of Angiogenic Factors

Mechanical characterization of polysaccharide/polyaminoacid hydrogels as potential scaffolds for tissue regeneration

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