Scaffolding polymeric biomaterials: Are naturally occurring biological macromolecules more appropriate for tissue engineering?

Abbasian, Mojtaba; Massoumi, Bakhshali; Mohammad‐Rezaei, Rahim; Samadian, Hadi; Jaymand, Mehdi

doi:10.1016/j.ijbiomac.2019.04.197

Cited by 164 publications

(89 citation statements)

References 270 publications

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“…Scaffold design is one of the essential aspects of tissue engineering because the nanofibers should be specialized in supporting attachment and proliferation of cells. To fabricating perfect scaffold for desired approach, many properties should be considered such as biodegradability, porosity and nanofiber diameters . In this study, we developed our scaffold based on our previous study but in new manner to increase percentage of CS up to 20%.…”

Section: Discussionmentioning

confidence: 99%

Chondro‐inductive nanofibrous scaffold based gelatin/polyvinyl alcohol/chondroitin sulfate for cartilage tissue engineering

Irani

Honarpardaz

Choubini

et al. 2020

Polymers for Advanced Techs

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Tissue engineering is a technique that can be used for repairing damaged cartilage.The aim of this study was chondro-differentiation of mesenchymal stem cells (MSCs) on polyvinyl alcohol/gelatin/chondroitin sulfate (PVA/GT/CS) blend nanofibers. Nanofibers were fabricated by electrospinning method with different concentrations of CS (10%, 15%, and 20%). MSCs were seeded on nanofibers for biocompatibility and cell viability test by MTT assay. Alcian blue staining assay was done to show that MSCs were differentiated to chondrocyte by staining the glycosaminoglycans (GAGs) that was produced by cells. Reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry (ICC) assay were done to confirm type II collagen expression. Scaffolds with different concentrations of CS were tested for biocompatibility, and the nanofiber with 15% of GAG showed better results (P = 0.02) compared with other nanofibers; therefore, it was chosen as the main sample for chondrogenesis tests. On the basis of scanning electron microscopy (SEM), hMSCs had a proper attachment to the nanofiber with 15% CS. Alcian blue staining showed the different rates of blue coloration, which means cells produced different types of GAGs from the GAG we used in the nanofiber. RT-PCR and ICC showed that COL2A1 was expressed in mRNA and protein levels. This study indicates that the PVA/GT/CS nanofiber containing 15% CS can be a good candidate for cartilage tissue engineering. K E Y W O R D S cartilage, chondroitin sulfate, electrospun, mesenchymal stem cells, tissue engineering

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Section: Discussionmentioning

confidence: 99%

Chondro‐inductive nanofibrous scaffold based gelatin/polyvinyl alcohol/chondroitin sulfate for cartilage tissue engineering

Irani

Honarpardaz

Choubini

et al. 2020

Polymers for Advanced Techs

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“…Bone tissue engineering has emerged in recent years as a potential approach to overcome the difficulties in healing, regeneration and functional restoration of bone defects following damages caused by trauma or as a result of osteoporosis amongst other relevant causes [1][2][3]. This approach is usually accompanied by the combination of a synthetic, natural or hybrid 2D or 3D scaffolds, cells, and a physiologically relevant chemical or physical signaling [4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Simple and Robust Fabrication and Characterization of Conductive Carbonized Nanofibers Loaded with Gold Nanoparticles for Bone Tissue Engineering Applications

Nekounam

Gholizadeh

Allahyari

et al. 2020

Preprint

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Bone tissue engineering is a new and applicable emerging approach to repair the bone defects. In this regard, designing and robust fabrication of tissue engineering scaffolds that could provide an appropriate environment for cell proliferation and differentiation is of high interest.Electrical conductive scaffolds which provide a substrate for stimulating cell growth and differentiation through a physiologically relevant physical signaling, electrical stimulation, has shown a highly promise in this approach. In this paper, we fabricated carbon nanofiber/gold nanoparticle (CNF/GNP) conductive scaffolds using two distinct methods; blending electrospinning in which gold nanoparticles were blended with electrospinning solution and electrospun, and electrospinning/electrospraying in which gold nanoparticle was electrosprayed simultaneously with electrospinning. The obtained electrospun mats underwent stabilization/carbonization process to prepare CNF/GNP scaffolds. The scaffolds were characterized by SEM, XRD, and Raman spectroscopy. SEM characterizations showed improved morphology and a slight decrease in the diameter of the spinned and sprayed nanofibers with moderate concentrations (from 178.66 ± 38.40 nm to 157.94 ± 24.14 nm and 120.81 ± 13.77 nm, respectively), In the electrosprayed form, better size distributions of nanofibers and less adhesion between individual fibers was observed, while XRD analysis confirmed the crystal structure of the nanofibers. Raman spectroscopy revealed enhancement in the graphitization of the structure, and the electrical conductivity of the structure improved by up to 29.2% and 81% in electrospraying and blending electrospinning modes, respectively. Indirect MTT and LDH toxicity assays directly were performed to assess MG63 cell toxicity, but no significant toxicity was observed and the scaffolds did not adversely affect cell proliferation.Overall, it can be concluded that in early tests, this structure have significant potential for bone tissue engineering applications.

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“…ECM-mimicking cell culture substrates are required for controlling cell function and fate as in vivo. However, synthetic polymers having a completely different composition from collagen have been used as artificial ECM [48]. Since gelatin is obtained from collagen and chemical composition is similar to collagen, gelatin hydrogel has attracted considerable attention as an artificial ECM.…”

Section: Introductionmentioning

confidence: 99%

Development of Advanced Biodevices Using Quantum Beam Microfabrication Technology

Oyama

Kimura

Nagasawa

et al. 2020

QuBS

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Biodevices with engineered micro- and nanostructures are strongly needed for advancements in medical technology such as regenerative medicine, drug discovery, diagnostic reagents, and drug delivery to secure high quality of life. The authors produced functional biocompatible plastics and hydrogels with physical and chemical properties and surface microscopic shapes that can be freely controlled in three dimensions during the production process using the superior properties of quantum beams. Nanostructures on a biocompatible poly(L-lactic acid) surface were fabricated using a focused ion beam. Soft hydrogels based on polysaccharides were micro-fabricated using a focused proton beam. Gelatin hydrogels were fabricated using γ-rays and electron beam, and their microstructures and stiffnesses were controlled for biological applications. HeLa cells proliferated three-dimensionally on the radiation-crosslinked gelatin hydrogels and, furthermore, their shapes can be controlled by the micro-fabricated surface of the hydrogel. Long-lasting hydrophilic concave structures were fabricated on the surface of silicone by radiation-induced crosslinking and oxidation. The demonstrated advanced biodevices have potential applications in three-dimensional cell culture, gene expression control, stem cell differentiation induction/suppression, cell aggregation into arbitrary shapes, tissue culture, and individual diagnosis in the medical field.

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Scaffolding polymeric biomaterials: Are naturally occurring biological macromolecules more appropriate for tissue engineering?

Cited by 164 publications

References 270 publications

Chondro‐inductive nanofibrous scaffold based gelatin/polyvinyl alcohol/chondroitin sulfate for cartilage tissue engineering

Chondro‐inductive nanofibrous scaffold based gelatin/polyvinyl alcohol/chondroitin sulfate for cartilage tissue engineering

Simple and Robust Fabrication and Characterization of Conductive Carbonized Nanofibers Loaded with Gold Nanoparticles for Bone Tissue Engineering Applications

Development of Advanced Biodevices Using Quantum Beam Microfabrication Technology

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