Scaffold Sheet Design Strategy for Soft Tissue Engineering

Tran, Richard T.; Thevenot, Paul; Zhang, Yi; Gyawali, Dipendra; Tang, Liping; Yang, Jian

doi:10.3390/ma3021375

Cited by 42 publications

(37 citation statements)

References 49 publications

(59 reference statements)

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“…Whole tissues in vivo may therefore have elastic moduli and ultimate tensile strengths approximately an order of magnitude greater than what is reported here 6 . For future tissue augmentation applications, several layers of constructs may potentially be combined to achieve the properties desired 42 .…”

Section: Discussionmentioning

confidence: 99%

A bioreactor system for in vitro tendon differentiation and tendon tissue engineering

Youngstrom

Rajpar

Kaplan

et al. 2015

Journal Orthopaedic Research

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There is significant clinical demand for functional tendon grafts in human and veterinary medicine. Tissue engineering techniques combining cells, scaffolds and environmental stimuli may circumvent the shortcomings of traditional transplantation processes. In this study, the influence of cyclic mechanical stimulation on graft maturation and cellular phenotype was assessed in an equine model. Decellularized tendon scaffolds from four equine sources were seeded with syngeneic bone marrow-derived mesenchymal stem cells and subjected to 0%, 3% or 5% strain at 0.33Hz for up to one hour daily for 11 days. Cells cultured at 3% strain integrated deep into their scaffolds, altered extracellular matrix composition, adopted tendon-like gene expression profiles, and increased construct elastic modulus and ultimate tensile strength to native levels. This bioreactor protocol is therefore suitable for cultivating replacement tendon material or as an in vitro model for studying differentiation of stem cells toward tendon.

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Section: Discussionmentioning

confidence: 99%

A bioreactor system for in vitro tendon differentiation and tendon tissue engineering

Youngstrom

Rajpar

Kaplan

et al. 2015

Journal Orthopaedic Research

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“…Advanced biomaterial technologies for creating artificial refined cell-instructive platforms based on knowledge obtained from materials science, biology and engineering have heralded a new era in the redesign of cellular scaffolds [37,96]. In this era, the architecture of the stem cell milieu or niche can be replicated in terms of its biochemical, mechanical, structural and component details to manipulate cell fate, including cell migration, gene expression and the maintenance of functional homeostasis [48].…”

Section: Biomaterials For Tissue Engineeringmentioning

confidence: 99%

“…More exciting than expected, a cohesive cell-formed sheet can be layered into 3D tissues or organs with physiological mechanical strength [715,716]; this concept has been demonstrated to be feasible in clinical sittings [717]. In addition, there is a wealth of evidence that cell-formed ECM products can be applied to enhance the large-scale expansion of highly functional adult human MSCs [718] or to decorate the surfaces of synthetic polymers and manufactured metals on which subsequent cell adhesion is regulated by adsorbed ECM proteins [96,184,719,720]. It is self-evident that the physical properties of synthetic biomaterials can be extensively tailored, but it is also clear that these materials often suffer from limited biological functionalities.…”

Section: Future Directions and Outlookmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

927

513

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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“…In the second, there is intensification of the inflammatory reaction and rejection of the material. The results showed that few lymphocytes were present, and did not show any cell destruction, though they did present a reaction mediated by giant cells 1,2,[30][31][32][33][34][35] .…”

Section: Figure 5 -Photomicrographs Of a Histological Analysis Of Thementioning

confidence: 99%

A comparative study of the areas of osteochondral defects produced in femoral condyles of rabbits treated with sugar cane biopolymer gel

Albuquerque

Aguiar²,

Filho³

et al. 2015

Acta Cir. Bras.

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PURPOSE:To assess the histological response of damaged osteochondral tissue in the femoral condyles of rabbits after repairing the wounds with sugar cane biopolymer gel -compared to the control group. METHODS:The study investigated 16 New Zealand rabbits, at 90, 120 and 180 days after surgery. In all the animals, a lesion of 3.2 mm in diameter and 4 mm deep was induced in each right and left femoral condyle. Each animal has provided both knees, divided into medial and lateral condyle, resulting in 64 samples. 32 knees were divided into two groups: Right knee, medial and lateral condyles, filled with biopolymer; Left knee, medial and lateral condyles, unfilled. The anatomical specimens were removed, and subjected to histological techniques and morphometric and statistical analysis. RESULTS:In all the periods of the group under study an inflammatory reaction mediated by giant cells and mononuclear cells was found, while in the control group there was early healing produced by fibroblasts and few mononuclear cells with statistical significance between groups. CONCLUSION:The biopolymer gel caused an inflammatory reaction mediated by giant cells and mononuclear cells while the control group there was cicatrization mediated by fibroblasts.Key words: Osteogenesis. Cartilage. Histology. Biopolymers. Rabbits.A comparative study of the areas of osteochondral defects produced in femoral condyles of rabbits treated with sugar cane biopolymer gel

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Scaffold Sheet Design Strategy for Soft Tissue Engineering

Cited by 42 publications

References 49 publications

A bioreactor system for in vitro tendon differentiation and tendon tissue engineering

A bioreactor system for in vitro tendon differentiation and tendon tissue engineering

Advancing biomaterials of human origin for tissue engineering

A comparative study of the areas of osteochondral defects produced in femoral condyles of rabbits treated with sugar cane biopolymer gel

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