Scaffold-free trachea regeneration by tissue engineering with bio-3D printing†

Taniguchi, Daisuke; Matsumoto, Keitaro; Tsuchiya, Tomoshi; Machino, Ryusuke; Takeoka, Yosuke; Elgalad, Abdelmotagaly; Gunge, Kiyofumi; Takagi, Katsunori; Taura, Yasuho; Hatachi, Go; Matsuo, Naoto; Yamasaki, Naoya; Nakayama, Koichi; Nagayasu, Takeshi

doi:10.1093/icvts/ivx444

Cited by 110 publications

(61 citation statements)

References 25 publications

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“…After a specific period of time, adjacent spheroids spontaneously fuse with each other and their 3D structure and form is maintained even when the needle array is decannulated (Moldovan et al, ). Tubular materials such as vessels (Itoh et al, ), tracheae (Taniguchi et al, ), and nerve conduits (Yurie et al, ; Zhang et al, ) have been fabricated using this “Bio‐3D printer.” The cell types used to form these structures have included fibroblasts, endothelial cells or mesenchymal stromal cells of human origin, thereby confirming that these structures can be created using a patient's own cells.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The Bio 3D conduits were fabricated by using a "Bio-3D printer (Regenova ® , Cyfuse, Tokyo, Japan)" to assemble the spheroids for constructing a tubular structure, as previously described (Itoh et al, 2015;Moldovan, Hibino, & Nakayama, 2017;Ong, Zhou, Han, et al, 2018;Takeoka, Matsumoto, Taniguchi, et al, 2019;Taniguchi et al, 2018;Yurie et al, 2017;Zhang et al, 2018). Briefly, spheroids were robotically picked out from the 96-well plate using a 25-gauge suction nozzle and inserted into a needle array unit according to a 3-dimensional computer-aided design that specified the shape of the tubular construct ( Figure 1b).…”

Section: Spheroid Generation and Fabrication Of Bio 3d Conduitsmentioning

confidence: 99%

“…A “Bio‐3D printer (Regenova®, Cyfuse, Tokyo, Japan)” can fabricate tubular materials such as vessels (Itoh, Nakayama, Noguchi, et al, ), tracheae (Taniguchi, Matsumoto, Tsuchiya, et al, ), and nerve conduits (Yurie, Ikeguchi, Aoyama, et al, ; Zhang et al, ) exclusively from cells without added foreign materials. The Bio 3D nerve conduit was fabricated by placing spheroids, which were generated from normal human dermal fibroblasts, into a needle array to form a tubular structure using a “Bio‐3D printer.” Biomaterial was not used during its fabrication.…”

Section: Introductionmentioning

confidence: 99%

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A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

et al. 2019

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Introduction A Bio 3D printed nerve conduit was reported to promote nerve regeneration in a 5 mm nerve gap model. The purpose of this study was to fabricate Bio 3D nerve conduits suitable for a 10 mm nerve gap and to evaluate their capacity for nerve regeneration in a rat sciatic nerve defect model. Materials and Methods Eighteen F344 rats with immune deficiency (9–10 weeks old; weight, 200–250 g) were divided into three groups: a Bio 3D nerve conduit group (Bio 3D, n = 6), a nerve graft group (NG, n = 6), and a silicon tube group (ST, n = 6). A 12‐mm Bio 3D nerve conduit or silicon tube was transplanted into the 10‐mm defect of the right sciatic nerve. In the nerve graft group, reverse autografting was performed with an excised 10‐mm nerve segment. Assessments were performed at 8 weeks after the surgery. Results In the region distal to the suture site, the number of myelinated axons in the Bio 3D group were significantly larger compared with the silicon group (2,548 vs. 950, p < .05). The myelinated axon diameter (MAD) and the myelin thickness (MT) of the regenerated axons in the Bio 3D group were significantly larger compared with those of the ST group (MAD: 3.09 vs. 2.36 μm; p < .01; MT: 0.59 vs. 0.40 μm, p < .01). Conclusions This study indicates that a Bio 3D nerve conduit can enhance peripheral nerve regeneration even in a 10 mm nerve defect model.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Spheroid Generation and Fabrication Of Bio 3d Conduitsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

et al. 2019

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“…Taniguchi et al . generated a scaffold‐free tubular trachea via 3D bioprinting using multicellular spheroids. Upon printing, the artificial trachea was matured in a bioreactor and transplanted into nine F334 rats.…”

Section: Organ and Tissue Biofabrificationmentioning

confidence: 99%

Emerging technologies in organ preservation, tissue engineering and regenerative medicine: a blessing or curse for transplantation?

et al. 2019

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Summary Since the beginning of transplant medicine in the 1950s, advances in surgical technique and immunosuppressive therapy have created the success story of modern organ transplantation. However, today more than ever, we are facing a huge discrepancy between organ supply and demand, limiting the potential for transplantation to save and improve the lives of millions. To address the current limitations and shortcomings, a variety of emerging new technologies focusing on either maximizing the availability of organs or on generating new organs and organ sources hold great potential to eventully overcoming these hurdles. These advances are mainly in the field of regenerative medicine and tissue engineering. This review gives an overview of this emerging field and its multiple sub‐disciplines and highlights recent advances and existing limitations for widespread clinical application and potential impact on the future of transplantation.

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Bioengineering and Clinical Translation of Human Lung and its Components

et al. 2023

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Clinical lung transplantation has rapidly established itself as the gold standard of treatment for end‐stage lung diseases in a restricted group of patients since the first successful lung transplant occurred. Although significant progress has been made in lung transplantation, there are still numerous obstacles on the path to clinical success. The development of bioartificial lung grafts using patient‐derived cells may serve as an alternative treatment modality; however, challenges include developing appropriate scaffold materials, advanced culture strategies for lung‐specific multiple cell populations, and fully matured constructs to ensure increased transplant lifetime following implantation. This review highlights the development of tissue‐engineered tracheal and lung equivalents over the past two decades, key problems in lung transplantation in a clinical environment, the advancements made in scaffolds, bioprinting technologies, bioreactors, organoids, and organ‐on‐a‐chip technologies. The review aims to fill the lacuna in existing literature toward a holistic bioartificial lung tissue, including trachea, capillaries, airways, bifurcating bronchioles, lung disease models, and their clinical translation. Herein, the efforts are on bridging the application of lung tissue engineering methods in a clinical environment as it is thought that tissue engineering holds enormous promise for overcoming the challenges associated with the clinical translation of bioengineered human lung and its components.

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Scaffold-free trachea regeneration by tissue engineering with bio-3D printing†

Abstract: The scaffold-free isogenic artificial tracheas produced by a bio-3D printer could be utilized as tracheal grafts in rats.

Cited by 110 publications

References 25 publications

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

Emerging technologies in organ preservation, tissue engineering and regenerative medicine: a blessing or curse for transplantation?

Bioengineering and Clinical Translation of Human Lung and its Components

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