Scaffold-free, stem cell-based cartilage repair

Yasui, Yukihiko; Ando, Wataru; Shimomura, Kazunori; Koizumi, Keiichi; Chijimatsu, Ryota; Hamamoto, Shuichi; Kobayashi, Masato; Yoshikawa, Hideki; Nakamura, Norimasa

doi:10.1016/j.jcot.2016.06.002

Cited by 35 publications

(26 citation statements)

References 75 publications

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“…In this regard, human synovium‐derived stem cells (hSSCs) have been identified as a good cell source based on their superior potential for chondrogenesis and proliferation compared with other adult stem cells in vitro (Sakaguchi, Sekiya, Yagishita, & Muneta, ). We have been developing several strategies for cartilage repair using hSSCs in vivo (Shimomura et al, ; Yasui, Ando, et al, ) and, moreover, have shown successful formation of cartilage‐like tissue from hSSCs in vitro (Chijimatsu et al, ; Koizumi et al, ; Yasui, Chijimatsu, et al, ). Although it will be potential alternatives for ACI, the cost to induce chondrogenesis using growth factors is not a negligible issue for clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro

Chijimatsu

Yano

Saito

et al. 2019

J Tissue Eng Regen Med

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Section: Introductionmentioning

confidence: 99%

Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro

Chijimatsu

Yano

Saito

et al. 2019

J Tissue Eng Regen Med

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“…[28]. In addition, there are some comparable approaches which focus on the production of SFCCs [9,11,[29][30][31]. SFCCs revealed a cartilage-like phenotype detected by gene expression and histological analysis (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Most 3D culture systems involve a scaffold to provide the cells with a predetermined structure where their distinct effects on the cells are often not considered. Recent developments aim to establish scaffold-free tissue engineered cartilage mainly from human chondrocytes which could serve as a promising approach to cartilage repair in vivo, as well as an excellent in vitro model, since ECM formation and degradation can be evaluated without the interference of a scaffold [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in silico modeling of cellular and matrix-related changes during the early phase of osteoarthritis

Weber

Fischer

Damerau

et al. 2019

Preprint

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Objective: Understanding the pathophysiological processes of osteoarthritis (OA) require adequate model systems. Although different in vitro or in vivo models have been described, further comprehensive approaches are needed to study specific parts of the disease. This study aimed to combine in vitro and in silico modeling to describe cellular and matrix-related changes during the early phase of OA. We developed an in vitro OA model based on scaffoldfree cartilage-like constructs (SFCCs), which was mathematically modeled using a partial differential equation (PDE) system to resemble the processes during the onset of OA.Design: SFCCs were produced from mesenchymal stromal cells and analyzed weekly by histology and qPCR to characterize the cellular and matrix-related composition. To simulate the early phase of OA, SFCCs were treated with interleukin-1β (IL-1β), tumor necrosis factor α (TNFα) and examined after 3 weeks or cultivated another 3 weeks without inflammatory cytokines to validate the regeneration potential. Mathematical modeling was performed in parallel to the in vitro experiments.Results: SFCCs expressed cartilage-specific markers, and after stimulation an increased expression of inflammatory markers, matrix degrading enzymes, a loss of collagen II (Col-2) and a reduced cell density was observed which could be partially reversed by retraction of stimulation. Based on the PDEs, the distribution processes within the SFCCs, including those of IL-1β, Col-2 degradation and cell number reduction was simulated. Conclusions:By combining in vitro and in silico methods, we aimed to develop a valid, efficient alternative approach to examine and predict disease progression and new therapeutic strategies.

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“…Such autologous synovial MSCs were applied to cartilage injury 29. Currently, only one clinical study repairing chondral defect using scaffold-free three-dimensional tissue engineered construct, derived from human synovial MSCs, was completed in March 2015; but, the outcomes of the study were not reported 30,31. This may be considered to be evident that these naturally occurring MSCs may play an important role in meniscal healing and regeneration.…”

Section: Introductionmentioning

confidence: 99%

Potential use of mesenchymal stem cells in human meniscal repair: current insights

Pak

Lee²,

Park³

et al. 2017

OAJSM

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The menisci of the human knee play an important role in maintaining normal functions to provide stability and nutrition to the articular cartilage, and to absorb shock. Once injured, these important structures have very limited natural healing potential. Unfortunately, the traditional arthroscopic meniscectomy performed on these damaged menisci may predispose the joint toward early development of osteoarthritis. Although a very limited number of studies are available, mesenchymal stem cells (MSCs) have been investigated as an alternative therapeutic modality to repair human knee meniscal tears. This review summarizes the results of published applications of MSCs in human patients, which showed that the patients who received MSCs (autologous adipose tissue-derived stem cells or culture-expanded bone marrow-derived stem cells) presented symptomatic improvements, along with magnetic resonance imaging evidences of the meniscal repair.

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Scaffold-free, stem cell-based cartilage repair

Cited by 35 publications

References 75 publications

Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro

Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro

In vitro and in silico modeling of cellular and matrix-related changes during the early phase of osteoarthritis

Potential use of mesenchymal stem cells in human meniscal repair: current insights

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