Scaffold-Free Cartilage Fabrication System Using Passaged Porcine Chondrocytes and Basic Fibroblast Growth Factor

Jin, Ri Long; Park, Sora; Choi, Byung Hyune; Min, Byoung‐Hyun

doi:10.1089/ten.tea.2008.0349

Cited by 22 publications

(14 citation statements)

References 44 publications

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“…24 Porcine chondrocyte doubling time in 2D culture is around 6.4 ± 0.3 days in serum-containing media. 41 We speculate that part of the reason the cells did not double at a similar rate when encapsulated in the PEG gels is that the selected gel formulations are non-degradable. Thus, the polymer network limited the amount of space available for chondrocytes to grow, and the media did not contain serum.…”

Section: Discussionmentioning

confidence: 92%

Covalently tethered TGF‐ β 1 with encapsulated chondrocytes in a PEG hydrogel system enhances extracellular matrix production

Sridhar

Doyle

Randolph

et al. 2014

J. Biomed. Mater. Res.

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Healing articular cartilage defects remains a significant clinical challenge because of its limited capacity for self-repair. While delivery of autologous chondrocytes to cartilage defects has received growing interest, combining cell-based therapies with growth factor delivery that can locally signal cells and promote their function is often advantageous. We have previously shown that PEG thiol-ene hydrogels permit covalent attachment of growth factors. However, it is not well known if embedded chondrocytes respond to tethered signals over a long period. Here, chondrocytes were encapsulated in PEG hydrogels functionalized with transforming growth factor-beta 1 (TGF-β1) with the goal of increasing proliferation and matrix production. Tethered TGF-β1 was found to be distributed homogenously throughout the gel, and its bioactivity was confirmed with a TGF-β1 responsive reporter cell line. Relative to solubly delivered TGF-β1, chondrocytes presented with immobilized TGF-β1 showed significantly increased DNA content and GAG and collagen production over 28 days, while maintaining markers of articular cartilage. These results indicate the potential of thiol-ene chemistry to covalently conjugate TGF-β1 to PEG to locally influence chondrocyte function over 4 weeks. Scaffolds with other or multiple tethered growth factors may prove broadly useful in the design of chondrocyte delivery vehicles for cartilage tissue engineering applications.

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Section: Discussionmentioning

confidence: 92%

Covalently tethered TGF‐ β 1 with encapsulated chondrocytes in a PEG hydrogel system enhances extracellular matrix production

Sridhar

Doyle

Randolph

et al. 2014

J. Biomed. Mater. Res.

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show abstract

“…Fibroblasts and chondrocytes, for example, produce collagens and proteoglycans in vitro. This ECM production capacity is accelerated under confluence or 3D culture conditions, a phenomenon that may be inspired to develop scaffold-free systems for fabrication of 3D constructs from isolated chondrocytes [73,74]. Normal anchorage-dependent cells proliferate at low density in monolayer culture.…”

Section: In Vitro Self-produced Ecm-rich Scaffold-free Constructsmentioning

confidence: 99%

In Vitro Biofabrication of Tissues and Organs

Nakayama

2013

Biofabrication

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“…Scaffold-free methods have been described in various platforms ranging from spheroid aggregates 5,7,8 to cartilage sheets. Scaffold-free cartilage sheet generation has been described from rat, 9 bovine, 10–12 goat, 13 pig, 14 rabbit, 15,16 and human 4 articular chondrocyte cell sources, and human mesenchymal stem cell sources.…”

Section: Introductionmentioning

confidence: 99%

Methods for Producing Scaffold-Free Engineered Cartilage Sheets from Auricular and Articular Chondrocyte Cell Sources and Attachment to Porous Tantalum

Whitney

Mera²,

Weidenbecher

et al. 2012

BioResearch Open Access

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Scaffold-free cartilage engineering techniques may provide a simple alternative to traditional methods employing scaffolds. We previously reported auricular chondrocyte-derived constructs for use in an engineered trachea model; however, the construct generation methods were not reported in detail. In this study, methods for cartilage construct generation from auricular and articular cell sources are described in detail, and the resulting constructs are compared for use in a joint resurfacing model. Attachment of cartilage sheets to porous tantalum is also investigated as a potential vehicle for future attachment to subchondral bone. Large scaffold-free cartilage constructs were produced from culture-expanded chondrocytes from skeletally mature rabbits, and redifferentiated in a chemically-defined culture medium. Auricular constructs contained more glycosaminoglycan (39.6±12.7 vs. 9.7±1.9 μg/mg wet weight, mean and standard deviation) and collagen (2.7±0.45 vs. 1.1±0.2 μg/mg wet weight, mean and standard deviation) than articular constructs. Aggregate modulus was also higher for auricular constructs vs. articular constructs (0.23±0.07 vs. 0.12±0.03 MPa, mean and standard deviation). Attachment of constructs to porous tantalum was achieved by neocartilage ingrowth into tantalum pores. These results demonstrate that large scaffold-free neocartilage constructs can be produced from mature culture-expanded chondrocytes in a chemically-defined medium, and that these constructs can be attached to porous tantalum.

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Scaffold-Free Cartilage Fabrication System Using Passaged Porcine Chondrocytes and Basic Fibroblast Growth Factor

Cited by 22 publications

References 44 publications

Covalently tethered TGF‐ β 1 with encapsulated chondrocytes in a PEG hydrogel system enhances extracellular matrix production

Covalently tethered TGF‐ β 1 with encapsulated chondrocytes in a PEG hydrogel system enhances extracellular matrix production

In Vitro Biofabrication of Tissues and Organs

Methods for Producing Scaffold-Free Engineered Cartilage Sheets from Auricular and Articular Chondrocyte Cell Sources and Attachment to Porous Tantalum

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