SC8 transcription assays for evaluating estrogenic activity

Detailed Review Paper Appraisal of Test Methods for Sex Hormone Disrupting Chemicals

“…In an abstract, von Angerer et al (1994) described the use of MCF-7 cells transiently transfected with a reporter plasmid containing the luciferase gene under the control of an oestrogen response element for evaluating the oestrogenic activity of various compounds. A further paper by the same authors used these transiently transfected MCF-7 cells to test the effects of an oestrogenic drug (Meyer et al, 1994). In a 1995 paper, Desauliniers et al compared various proliferation indices of MCF-7 cells (including the MTT assay, […”

“…The authors used this system to detect oestrogenic and anti-oestrogenic activities. Using apparently similar cells, von Angerer et al (1994) were unable to detect weak oestrogens (agonists) because of a high level of basal luciferase activity. In contrast, Pons et al found the system to be highly sensitive and suggested the assay could be used as an early screening test for new oestrogens or anti-oestrogens for a number of reasons: a) Luciferase was expressed in response to oestradiol exposure in a dose-dependent manner with an EC 50 lower than 10 -10 M.…”

Ligand‐free, copper‐catalyzed, one‐pot, three‐component synthesis of novel 1,2,3‐triazole‐linked indoles in magnetized water

“…Indole is an important material used in organic synthesis, and its physiological properties have attracted much scientific attention . A variety of 2‐phenylindoles have been found to inhibit the growth of human breast cancer cells by dissimilar mechanisms depending upon the type and position of the substituents . Recently, 2‐phenylindole‐3‐carboxaldehydes have been proven to exert antimitotic activity in human breast cancer cells by inhibiting tubulin polymerization .…”