SC1 limits tube formation, branching, migration, expansion and induce apoptosis of endothelial cells

Akgöl, Sezer; Kalkan, Batuhan Mert; Yücel, Doğacan; Kocabaş, Fatih

doi:10.1016/j.vph.2021.106903

Cited by 2 publications

(2 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The matrigel tube formation assay is widely used to evaluate angiogenesis in in vitro experiments 20,21 . Matrigel solution (ABW, China, Cat.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Fucoidan promotes angiogenesis and accelerates wound healing through AKT/Nrf2/HIF‐1α signalling pathway

Wen,

Yang,

Wang

et al. 2023

International Wound Journal

View full text Add to dashboard Cite

After skin injury, wound repair involves a complex process in which angiogenesis plays a crucial role. Previous research has indicated that fucoidan may aid in wound healing; we therefore hypothesised that fucoidan may speed up the process by promoting angiogenesis. In this study, we investigated the potential molecular mechanism underlying fucoidan's ability to accelerate wound healing by promoting angiogenesis. Using a full‐cut wound model, we observed that fucoidan significantly intensified wound closure and promoted granulation formation and collagen deposition. Immunofluorescence staining revealed that fucoidan also promoted wound angiogenesis, specifically by accelerating the migration of new blood vessels to the middle area of the wound. Furthermore, fucoidan demonstrated the ability to enhance the proliferation of human umbilical vein endothelial cells (HUVECs) damaged by hydrogen peroxide (H2O2) and to improve the formation of endothelial tubes. Mechanistic studies revealed that fucoidan upregulated the protein levels of the AKT/Nrf2/HIF‐1α signalling pathway, which plays a crucial role in angiogenesis. This was further confirmed using the inhibitor LY294002, which reversed the promotion of endothelial tube formation by fucoidan. Overall, our findings suggest that fucoidan can promote angiogenesis via the AKT/Nrf2/HIF‐1α signalling pathway and accelerate wound healing.

show abstract

“…The matrigel tube formation assay is widely used to evaluate angiogenesis in in vitro experiments 20,21 . Matrigel solution (ABW, China, Cat.…”

Section: Methodsmentioning

confidence: 99%

“…The matrigel tube formation assay is widely used to evaluate angiogenesis in in vitro experiments. 20,21 Matrigel solution (ABW, China, Cat. 0827045) was thawed overnight at 4 C and placed in pre-refrigerated 48-well plates on ice.…”

Section: In Vitro Tube Formation Assaymentioning

confidence: 99%