SC1/Hevin

Hambrock, Harald O.; Nitsche, D. Patric; Hansen, Uwe; Brückner, Peter; Paulsson, Mats; Maurer, Patrik; Hartmann, Ursula

doi:10.1074/jbc.m212291200

Cited by 76 publications

(35 citation statements)

References 29 publications

(29 reference statements)

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“…Sparcl1, which was also overexpressed in purified BFCN, is highly expressed in the developing and adult CNS and contains one follistatin domain (49); thus, like noggin, it may interact with BMPs. The rise in Sparcl1 expression upon treatment with BMP9, preceding those of Col1a1 and Hspb8, may not be surprising because SPARC (secreted protein, acidic, cysteine-rich) can bind to collagen type I and regulate its production (50). In this regard, it should be pointed out that BMP9 induced the concerted expression of genes associated with the laying down of extracellular collagen matrix.…”

Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein 9 induces the transcriptome of basal forebrain cholinergic neurons

López-Coviella

Follettie

Mellott

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Basal forebrain cholinergic neurons (BFCN) participate in processes of learning, memory, and attention. Little is known about the genes expressed by BFCN and the extracellular signals that control their expression. Previous studies showed that bone morphogenetic protein (BMP) 9 induces and maintains the cholinergic phenotype of embryonic BFCN. We measured gene expression patterns in septal cultures of embryonic day 14 mice and rats grown in the presence or absence of BMP9 by using species-specific microarrays and validated the RNA expression data of selected genes by immunoblot and immunocytochemistry analysis of their protein products. BMP9 enhanced the expression of multiple genes in a time-dependent and, in most cases, reversible manner. The set of BMP9-responsive genes was concordant between mouse and rat and included genes encoding cell-cycle͞growth control proteins, transcription factors, signal transduction molecules, extracellular matrix, and adhesion molecules, enzymes, transporters, and chaperonins. BMP9 induced the p75 neurotrophin receptor (NGFR), a marker of BFCN, and Cntf and Serpinf1, two trophic factors for cholinergic neurons, suggesting that BMP9 creates a trophic environment for BFCN. To determine whether the genes induced by BMP9 in culture were constituents of the BFCN transcriptome, we purified BFCN from embryonic day 18 mouse septum by using fluorescence-activated cell sorting of NGFR ؉ cells and profiled mRNA expression of these and NGFR ؊ cells. Approximately 30% of genes induced by BMP9 in vitro were overexpressed in purified BFCN, indicating that they belong to the BFCN transcriptome in situ and suggesting that BMP signaling contributes to maturation of BFCN in vivo.nerve growth factor receptor ͉ neuronal development ͉ septum ͉ microarray ͉ fluorescence-activated cell sorting B one morphogenetic proteins (BMPs) play critical roles in the development of the nervous system, and there is growing evidence that BMPs regulate the expression of neurotransmitter phenotype, including the cholinergic phenotype (1-4) of basal forebrain cholinergic neurons (BFCN) that project to the neocortex and hippocampus and are important in the processes of attention, learning, and memory (5). Previous studies identified multiple BMP-regulated target genes in a variety of cells, including nervous tissue. However, although BMP target genes have been characterized in the specification of catecholaminergic and serotonergic neurons (6, 7), little is known about BMP target genes implicated in BFCN determination. BFCN are defined by their neuroanatomical location and the neurotransmitter that they synthesize and release, i.e., acetylcholine. The latter process requires a concerted expression of three genes, encoding choline acetyltransferase (Chat), the vesicular acetylcholine transporter (Vacht), and the choline transporter 1 (Cht1). Several additional features of these cells include expression of acetylcholinesterase, the neurotrophin receptors NGFR and TRKA, the expression of certain neurotransmitter receptors (...

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Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein 9 induces the transcriptome of basal forebrain cholinergic neurons

López-Coviella

Follettie

Mellott

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…This hypothesis had been demonstrated by many researches. Hambrock et al reported SPARCL1 in the extracellular matrix (ECM) tethers to the collagen ECM [28]. Hurley et al and his colleagues demonstrate that SPARCL1 in the ECM limited cellular ability to form focal adhesions on the collagen matrix and thereby attenuated corresponding biophysical dynamics of the cytoskeletal framework, which have been shown to potentiate migration [29].…”

Section: Discussionmentioning

confidence: 99%

SPARCL1, a Novel Prognostic Predictive Factor for GI Malignancies: a Meta-Analysis

Cui

Zheng

et al. 2017

Cell Physiol Biochem

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Background/Aims: Secreted protein acidic and rich in cysteines-like 1 (SPARCL1) is abnormally expressed in gastrointestinal (GI) malignancies. However, the correlation between SPARCL1 expression and the prognosis of patients remains unknown. Therefore, we performed a meta-analysis to investigate the potential value of SPARCL1 as a prognostic predictive marker for GI malignancies. Methods: The PubMed, Embase, EBSCO, CNKI, and Wanfang databases were systematically searched for studies examining SPARCL1 and clinicopathological features, including the prognoses of patients. Hazard ratios (HRs) and odds ratios (ORs) from individual studies were calculated and pooled using a random-effects or fix-effects model. Heterogeneity and publication bias analyses were performed. Results: Data from 8 studies, including a total of 2,356 patients, were summarized. The expression of SPARCL1 suggested a better prognosis (HR=0.57, 95% CI: 0.445-0.698, P=0.000) and was associated with clinicopathological features of GI malignancies, including distant metastasis (OR=0.44, 95% CI: 0.23-0.85, P=0.014), lymph node metastasis (OR=0.56, 95% CI: 0.39–0.81, P=0.002) and tumor differentiation (OR=2.21, 95% CI: 1.82–2.69, P=0.000). Subgroup analyses based on cancer type revealed that the expression of SPARCL1 had no effect on lymph node metastasis in colorectal cancer, and it did not influence tumor differentiation in gastric cancer. Egger’s test showed no evidence of publication bias (all P>0.05). Conclusion: SPARCL1 could be a novel prognostic predictive factor for GI malignancies. The expression of SPARCL1 could influence the clinicopathological features of GI malignancies. Further large-scale studies are essential to confirm SPARCL1’s prognostic predictive value, and more fundamental experimental studies are needed to illustrate the mechanisms.

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“…33 SPRL1 is also known to bind collagen-I 34 and can regulate decorin production and collagen assembly. 35 In cancer, the role of SPRL1 remains poorly understood.…”

Section: ■ Discussionmentioning

confidence: 99%

Sparc-Like Protein 1 Is a New Marker of Human Glioma Progression

et al. 2012

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High-grade gliomas (glioblastomas) are the most common and deadly brain tumors in adults, currently with no satisfactory treatment available. Apart from de novo glioblastoma, it is currently accepted that these malignancies mainly progress from lower grade glial tumors. However, the molecular entities governing the progression of gliomas are poorly understood. Extracellular and membrane proteins are key biomolecules found at the cell-to-cell communication interface and hence are a promising proteome subpopulation that could help understand the development of glioma. Accordingly, the current study aims at identifying new protein markers of human glioma progression. For this purpose, we used glial tumors generated orthotopically with T98G and U373 human glioma cells in nude mice. This setup allowed also to discriminate the protein origin, namely, human (tumor) or mouse (host). Extracellular and membrane proteins were selectively purified using biotinylation followed by streptavidin affinity chromatography. Isolated proteins were digested and then identified and quantified employing 2D-nano-HPLC−MS/MS analysis. A total of 23 and 27 upregulated extracellular and membrane proteins were identified in the T98G and U373 models, respectively. Approximately two-thirds of these were predominantly produced by the tumor, whereas the remaining proteins appeared to be mainly overexpressed by the host tissue. Following extensive validation, we have focused our attention on sparc-like protein 1. This protein was further investigated using immunohistochemistry in a large collection of human glioma samples of different grades. The results showed that sparc-like protein 1 expression correlates with glioma grade, suggesting the possible role for this protein in the progression of this malignancy.

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SC1/Hevin

Cited by 76 publications

References 29 publications

Bone morphogenetic protein 9 induces the transcriptome of basal forebrain cholinergic neurons

Bone morphogenetic protein 9 induces the transcriptome of basal forebrain cholinergic neurons

SPARCL1, a Novel Prognostic Predictive Factor for GI Malignancies: a Meta-Analysis

Sparc-Like Protein 1 Is a New Marker of Human Glioma Progression

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