SBTX, a new toxic protein distinct from soyatoxin and other toxic soybean [Glycine max] proteins, and its inhibitory effect on Cercospora sojina growth

Vasconcelos, Ilka M.; Morais, Janne K. S.; Siebra, Elisângela A; Carlini, Célia R.; Sousa, Daniele O.B.; Beltramini, Leila Maria; Melo, Vânia Maria Maciel; Oliveira, José T.A.

doi:10.1016/j.toxicon.2007.10.005

Cited by 11 publications

(14 citation statements)

References 33 publications

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“…Therefore, for experiments involving treatment with the protein, a four-fold diluted SDB was used, allowing the protein to be fully active without significantly affecting the growth of any of the C. albicans strains used for this study. SBTX was purified as described previously [1]. Briefly, soybean defatted powder was extracted with 0.025 M Tris-HCl/0.005 M dithiothreitol (DTT), pH 7.5, (1∶5, m/v) for 3 h at 4°C under constant stirring and filtered through cheesecloth.…”

Section: Methodsmentioning

confidence: 99%

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Soybean Toxin (SBTX) Impairs Fungal Growth by Interfering with Molecular Transport, Carbohydrate/Amino Acid Metabolism and Drug/Stress Responses

et al. 2013

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Soybean toxin (SBTX) is an antifungal protein from soybeans with broad inhibitory activity against the growth and filamentation of many fungi, including human and plant pathogenic species such as Candida albicans, Candida parapsilosis, Aspergillus niger, Penicillium herquei, Cercospora sojina and Cercospora kikuchii. Understanding the mechanism by which SBTX acts on fungi and yeasts may contribute to the design of novel antifungal drugs and/or the development of transgenic plants resistant to pathogens. To this end, the polymorphic yeast C. albicans was chosen as a model organism and changes in the gene expression profile of strain SC5314 upon exposure to SBTX were examined. Genes that were differentially regulated in the presence of SBTX were involved in glucose transport and starvation-associated stress responses as well as in the control of both the induction and repression of C. albicans hyphal formation. Transmission electron microscopy showed that C. albicans cells exposed to SBTX displayed severe signs of starvation and were heavily granulated. Our data were indicative of C. albicans cell starvation despite sufficient nutrient availability in the medium; therefore, it can be speculated that SBTX blocks nutrient uptake systems. Because neither the starvation signal nor the alkaline response pathway lead to the induction of hyphae, we hypothesise that conflicting signals are transmitted to the complex regulatory network controlling morphogenesis, eventually preventing the filamentation signal from reaching a significant threshold.

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Section: Methodsmentioning

confidence: 99%

“…kg -1 by intraperitoneal injection). We have previously shown that SBTX inhibits the mycelial growth of several phytopathogenic species, including Cercospora sojina and C. kikuchii [1]. Both of these fungi cause economically important diseases in soybeans [2], [3].…”

Section: Introductionmentioning

confidence: 99%

Soybean Toxin (SBTX) Impairs Fungal Growth by Interfering with Molecular Transport, Carbohydrate/Amino Acid Metabolism and Drug/Stress Responses

et al. 2013

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“…Lectins are another class of antinutritional elements that become attached to the brush layer of the intestinal epithelium, reducing absorption and causing atrophy of the microvilli. Soybean seeds can also contain toxic components, including ureases, soybean toxin (SBTX) and soyatoxin (SYTX) . The content and activity of these antinutritional and toxic factors have been extensively studied in varied geographic and genetically modified cultivars .…”

Section: ‘State‐of‐the‐art’ Seed Proteomicsmentioning

confidence: 99%

Section: Proteomic Strategies In Trait Improvement In Soybean Seedsmentioning

confidence: 99%

Proteomic insights into synthesis of isoflavonoids in soybean seeds

Dastmalchi

Dhaubhadel

2015

Proteomics

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Soybean seeds are the major human dietary source of isoflavonoids, a class of plant natural products almost entirely exclusive to legumes. Isoflavonoids reduce the risk of a number of chronic human illnesses. Biosynthesis and accumulation of this class of compounds is a multigenic and complex trait, with a great deal of variability among soybean cultivars and with respect to the environment. There is a wealth of genomic, transcriptomic, and metabolomics data regarding isoflavonoid biosynthesis, but the connection between multigene families and their cognate proteins is a missing link that could provide us with a great deal of functional information. The changing proteome of the developing seed can shed light on the correlative increase in isoflavonoids, while the maternal seed coat proteome can provide the link with inherited metabolic and signaling machinery. In this effort, 'seed-filling' proteomics has revealed key secondary metabolite enzymes that quantitatively vary throughout seed development. Seed coat proteomics has revealed the existence of metabolic apparatus specific to isoflavonoid biosynthesis (isoflavonoid reductase) that could potentially influence the chemical content of this organ. The future of proteomic analysis of isoflavonoid biosynthesis should be centered on the development of quantitative, tissue-specific proteomes that emphasize low-abundance metabolic proteins to extract the whole suite of factors involved.

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“…It inhibited the growth of Cercospora sojina, a fungus causing frogeye leaf spot in soybeans at a concentration much lower than the lethal dose to mice. Thus it may be useful for the development of transgenic plants with augmented resistance to pathogens [30].…”

Section: Sbtx a New Toxic Protein Distinct From Soyatoxinmentioning

confidence: 99%