Saving the zone of stasis in burns with melatonin: An experimental study in rats

Kayapınar, Muhammed; Seyhan, Nevra; Avunduk, Mustafa Cihad; Savacı, Nedim

doi:10.5505/tjtes.2015.53059

Cited by 3 publications

(4 citation statements)

References 28 publications

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“…In this study, we used the experimental model of zone of stasis, which was first described by Regas and Ehrlich (1,11). By calculating (a) the amount of living tissue macroscopically, (b) cell proliferation immunohistochemically by PCNA, and through (c) scintigraphic examination, we showed that the quercetin treatment, especially pre-burn, statistically significantly increased the viability of tissue in the zone of statis of a burn when compared to the control group.…”

Section: Discussionmentioning

confidence: 95%

“…After the appropriate antiseptic application, burns were created on the dorsum of each animal using a custommade steel comb with four rectangular rows (20x10 mm) and three interspaces (20x5 mm) weighing 150 gr pre-heated in boiling water for 15 minutes and applied without pressure for 20 seconds. This resulted in four rectangular 10×20 mm full-thickness burns separated by three 5×10 mm unburned interspaces representing the zone of stasis, according to the comb burn model defined by Regas and Ehrlich (11).…”

Section: Burn Proceduresmentioning

confidence: 99%

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Beneficial Effects of Quercetin on the Zone of Stasis in an Experimental Burn Model

Orhan¹,

Şahin²,

Metin³

et al. 2018

Erciyes Med J

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Objective: In burn injury, the zone of stasis determines the width and depth of the necrosis. Our aim is to show the effectiveness of quercetin on the viability within the zone of stasis in burns of rats. Materials and Methods: Forty-eight rats were divided into three groups. The rats in Group 1 (control group) were only applied the comb burn model; the rats in Group 2 (post-burn group) were administered 50 mg/kg of quercetin intraperitoneally, every day after the burn procedure until euthanasia; and the rats in Group 3 (pre-burn group) were administered 50 mg/kg of quercetin intraperitoneally, every day for 7 days before and after the burn procedure until euthanasia. Results: The living tissue calculated was 85.41% (±14.06) in Group 3, 40.37% (±9.75) in Group 2, and 16.81% (±9.4) in Group 1. The level of apoptosis was 30.0 (±10.8) in Group 3, 33.8 (±08.7) in Group 2, and 37.4 (±11.5) in Group 1. The level of autophagy was 49.50 (±8.58) in Group 3, 27.17 (±5.53) in Group 2, and 21.00 (±5.66) in Group 1. All the differences between the groups were statistically significant (p<0.01). Conclusion: Quercetin reduces apoptosis and increases autophagy, thereby increasing tissue viability in the zone of stasis of burn injury.

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Section: Discussionmentioning

confidence: 95%

Section: Burn Proceduresmentioning

confidence: 99%

Beneficial Effects of Quercetin on the Zone of Stasis in an Experimental Burn Model

Orhan¹,

Şahin²,

Metin³

et al. 2018

Erciyes Med J

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“…There is preliminary evidence that melatonin has benefits that limit burn wound damage. Data shows that, when given parentally, melatonin reduces cellular damage in the zone of stasis that surrounds the area of irreversible coagulation necrosis that is a result of a burn [152,153]. In particular, the histopathological examination of the zone of stasis revealed less edema and congestion, a reduced inflammatory response, and less fibrotic tissue generation, thereby limiting scar tissue.…”

Section: Melatonin In Burn Wound Damagementioning

confidence: 96%

The Multiple Functions of Melatonin: Applications in the Military Setting

Gancitano

Reíter

2022

Biomedicines

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The aim of this review is to provide the reader with a general overview on the rationale for the use of melatonin by military personnel. This is a technique that is being increasingly employed to manage growing psycho-physical loads. In this context, melatonin, a pleotropic and regulatory molecule, has a potential preventive and therapeutic role in maintaining the operational efficiency of military personnel. In battlefield conditions in particular, the time to treatment after an injury is often a major issue since the injured may not have immediate access to medical care. Any drug that would help to stabilize a wounded individual, especially if it can be immediately administered (e.g., per os) and has a very high safety profile over a large range of doses (as melatonin does) would be an important asset to reduce morbidity and mortality. Melatonin may also play a role in the oscillatory synchronization of the neuro–cardio–respiratory systems and, through its epigenetic action, poses the possibility of restoring the main oscillatory waves of the cardiovascular system, such as the Mayer wave and RSA (respiratory sinus arrhythmia), which, in physiological conditions, result in the oscillation of the heartbeat in synchrony with the breath. In the future, this could be a very promising field of investigation.

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“…MT can preserve the level of glutathione (GSH) within cells and in mitochondria, suppressing oxidative damage [42,43]. MT not only attenuates oxidative damage in experimental burns [44][45][46][47][48] but also in burn patients [49]. While previous research indicates that MT could be beneficial in instances of DOI after severe skin burn, a comprehensive review on this topic has yet to be performed.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Melatonin against Severe Burn-Induced Distant Organ Injury: A Systematic Review and Meta-Analysis of Experimental Studies

et al. 2020

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Extensive burns result in a local wound response and distant-organ injury (DOI) caused by oxidative-stress and inflammation. Melatonin (MT) shows promise in alleviating oxidative-stress and inflammation, but its role in thermal injury is largely unexplored. The present systematic review and meta-analysis were designed to assess the effects of MT on oxidative-stress and inflammatory markers against severe burn-induced DOI. Mean difference (MD)/standard mean difference (SMD) with 95% confidence interval (CI) were estimated using fixed-effect/random-effects models. Eighteen experimental studies met the inclusion criteria. Compared with the control group, MT significantly decreased the levels of malondialdehyde (SMD, −1.03; 95% CI, −1.30, −0.76, p < 0.00001) and 4-hydroxynonenal (MD, −1.06; 95% CI, −1.57, −0.56, p < 0.0001). Additionally, MT increased the levels of glutathione (SMD, 1.94; 95% CI, 1.27, 2.61, p < 0.00001) and superoxide-dismutase (SMD, 0.76; 95% CI, 0.08, 1.45, p = 0.03). Finally, MT significantly decreased the levels of tumor necrosis factor-α (SMD, −1.34; 95% CI, −1.92 to −0.77; p < 0.00001) and C-reactive protein (MD, −12.67; 95% CI, −16.72 to −8.62; p < 0.00001). Meta-analysis indicates that severe burn followed by immediate MT (10 mg/kg) intervention shows significant beneficial effects after 24-h against DOI by regulating oxidative-stress and the inflammatory response.

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Saving the zone of stasis in burns with melatonin: An experimental study in rats

Cited by 3 publications

References 28 publications

Beneficial Effects of Quercetin on the Zone of Stasis in an Experimental Burn Model

Beneficial Effects of Quercetin on the Zone of Stasis in an Experimental Burn Model

The Multiple Functions of Melatonin: Applications in the Military Setting

Protective Effects of Melatonin against Severe Burn-Induced Distant Organ Injury: A Systematic Review and Meta-Analysis of Experimental Studies

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