Sauroine — A Novel Lycopodium Alkaloid from Huperzia saururus.

Abstract

Alkaloids U 0600 Sauroine -A Novel Lycopodium Alkaloid from Huperzia saururus. -(ORTEGA, M. G.; AGNESE, A. M.; CABRERA*, J. L.; Tetrahedron Lett. 45 (2004) 38, 7003-7005; Dep. Farm., Fac. Cienc. Quim., Univ. Nac. Cordoba, 5016 Cordoba, Argent.; Eng.) -Mais 01-179

Huperzia saururus, activity on synaptic transmission in the hippocampus

“…In order to separate and identify the components present in E 2 this extract was submitted to GLC-MS (Ortega et al, 2004a). By using a Perkin-Elmer Qmass-910 apparatus, and a capillary column SE 30, 30 m in length, E 2 analysis was performed.…”

“…can produce severe adverse effects such as vomiting, diarrhea, convulsions and even death (Amorin, 1974;Ratera and Ratera, 1980;Toursarkissian, 1980). Results of previous studies on Huperzia saururus showed that its main constituents are alkaloids; sauroine, a novel alkaloid (Ortega et al, 2004a), sauroxine, 6-hydroxylycopodine, N-acetyllycodine, lycopodine, lycodine, N-methyllycodine, and clavolonine were isolated and identified (Ortega et al, 2004b) (Fig. 1).…”

Study of the interaction of Huperzia saururus Lycopodium alkaloids with the acetylcholinesterase enzyme

“…[43] Previous studies on H. saururus collected in Pampa de Achala (Córdoba), Argentina, showed the presence of eight different alkaloids in a purified alkaloid extract, with sauroine (9), being the predominant one (Scheme 2). [21,44] The purified alkaloid extract of H. saururus was shown to be active as an AChEI, [45,46] although the major alkaloid (9) was not. The IC 50 values for the Page 6 of 23 A c c e p t e d M a n u s c r i p t Lycopodine Class of alkaloids were similar to those of other alkaloids from the same class [47] but based on the experimental information available, the lycopodine type alkaloids may be less active than the lycodine type.…”

“…Sauroine (9), 6-hydroxylycopodine (10) and sauroxine (11) were isolated, purified and identified as previously reported. [21,[44][45][46]49] Anticholinesterase activity assay Enzyme source. Erythrocyte membranes were used as the enzyme source and were obtained as previously informed.…”

“…Erythrocyte membranes were used as the enzyme source and were obtained as previously informed. [21,[44][45][46] Determination of the inhibitory effect. The acetyl cholinesterase assay was performed using the colorimetric method of Ellman and co-workers, [50] with some modifications incorporated by our group.…”