SAUR15 Promotes Lateral and Adventitious Root Development via Activating H<sup>+</sup>-ATPases and Auxin Biosynthesis

Yin, Hengfu; Li, Mengzhan; Lv, Mei; Hepworth, Shelley R.; Li, Dingding; Ma, Chaofan; Li, Jia

doi:10.1104/pp.19.01250

Cited by 42 publications

(42 citation statements)

References 82 publications

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“…Indole‐3‐acetic acid stimulation of PR growth has been investigated in numerous studies, and there are two theories to explain how auxin promotes root growth: the ‘acid‐growth theory’ and the ‘gene expression theory’ (Rayle et al ., 1970; Takahashi et al ., 2012; Enders & Strader, 2015; Wang et al ., 2016; Yin et al ., 2020). Here, we demonstrate that free IAA can suppress NH 4 + efflux in roots under high NH 4 + (Fig.…”

Section: Discussionmentioning

confidence: 99%

WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and indole‐3‐acetic acid‐conjugating genes and by inhibiting ammonium efflux in the root elongation zone

Sun

Wang

et al. 2021

New Phytologist

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Summary Ammonium (NH4+) is toxic to root growth in most plants, even at moderate concentrations. Transcriptional regulation is one of the most important mechanisms in the response of plants to NH4+ toxicity, but the nature of the involvement of transcription factors (TFs) in this regulation remains unclear. Here, RNA‐seq analysis was performed on Arabidopsis roots to screen for ammonium‐responsive TFs. WRKY46, the member of the WRKY transcription factor family most responsive to NH4+, was selected. We defined the role of WRKY46 using mutation and overexpression assays, and characterized the regulation of NUDX9 and indole‐3‐acetic acid (IAA)‐conjugating genes by WRKY46 via yeast one‐hybrid and electrophoretic mobility shift assays and chromatin immunoprecipitation‐quantitative real‐time polymerase chain reaction (ChIP‐qPCR). Knockout of WRKY46 increased, while overexpression of WRKY46 decreased, NH4+‐suppression of the primary root. WRKY46 is shown to directly bind to the promoters of the NUDX9 and IAA‐conjugating genes (GH3.1, GH3.6, UGT75D1, UGT84B2) and to inhibit their transcription, thus positively regulating free IAA content and stabilizing protein N‐glycosylation, leading to an inhibition of NH4+ efflux in the root elongation zone (EZ). We identify TF involvement in the regulation of NH4+ efflux in the EZ, and show that WRKY46 inhibits NH4+ efflux by negative regulation of NUDX9 and IAA‐conjugating genes.

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Section: Discussionmentioning

confidence: 99%

WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and indole‐3‐acetic acid‐conjugating genes and by inhibiting ammonium efflux in the root elongation zone

Sun

Wang

et al. 2021

New Phytologist

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“…ARFs typically regulate the expression of auxin responsive genes through binding to auxin response elements (AuxRE: TGTCTC) in the promoter regions of the target genes (Ghelli et al ., 2018; Guilfoyle and Hagen, 2007; Yin et al ., 2020). To this end, an AuxRE was found at position −1549 (+) of the CWIN2 promoter (Figure 4a; Table S5).…”

Section: Discussionmentioning

confidence: 99%

Identification of transcription factors controlling cell wall invertase gene expression for reproductive development via bioinformatic and transgenic analyses

Foster

et al. 2021

The Plant Journal

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Cell wall invertase (CWIN) hydrolyses sucrose into glucose and fructose in the extracellular matrix and plays crucial roles in assimilate partitioning and sugar signalling. However, the molecular regulators controlling CWIN gene transcription remain unknown. As the first step to address this issue, we performed bioinformatic and transgenic studies, which identified a cohort of transcription factors (TFs) modulating CWIN gene expression in Arabidopsis thaliana. Comprehensive bioinformatic analyses identified 18 TFs as putative regulators of the expression of AtCWIN2 and AtCWIN4 that are predominantly expressed in Arabidopsis reproductive organs. Among them, MYB21, ARF6, ARF8, AP3 and CRC were subsequently shown to be the most likely regulators of CWIN gene expression based on molecular characterization of the respective mutant of each candidate TF. More specifically, the obtained data indicate that ARF6, ARF8 and MYB21 regulate CWIN2 expression in the anthers and CWIN4 in nectaries, anthers and petals, whereas AP3 and CRC were determined primarily to regulate the transcriptional activity of CWIN4. TF-promoter interaction assays demonstrated that ARF6 and ARF8 directly control CWIN2 and CWIN4 transcription with AP3 activating CWIN4. The involvement of ARF8 in regulating CWIN4 expression was further supported by the finding that enhanced CWIN4 expression partially recovered the short silique phenotype displayed by the arf8-3 mutant. The identification of the five TFs regulating CWIN expression serves as a launching pad for future studies to dissect the upstream molecular network underpinning the transcription of CWINs and provides a new avenue, potentially, to engineer assimilate allocation and reproductive development for improving seed yield.

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“…COI1-dependent JA signaling actives the expression of DAO1, which in turn regulates IAA homeostasis by irreversible degradation ( Lakehal et al, 2019b ). Copeland (2020) and Yin et al (2020) proposed a model of the involvement of SAUR15 in auxin signaling-mediated AR formation in Arabidopsis seedlings. ARFs directly bind to the promoter of SAUR15 to activate its expression.…”

Section: Molecular Bases Associated With Auxin Signaling During Advenmentioning

confidence: 99%

“…Recently, Yin et al (2020) revealed the interaction between SMALL AUXIN-UP RNA15 ( SAUR15 ) and ARFs in controlling AR formation. ARF6 and ARF7 directly bind to the promoter of SAUR15 to promote its expression.…”

Section: Molecular Bases Associated With Auxin Signaling During Advenmentioning

confidence: 99%

Molecular Bases for the Regulation of Adventitious Root Generation in Plants

2021

Front. Plant Sci.

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The formation of adventitious roots (ARs) is an ecologically and economically important developmental process in plants. The evolution of AR systems is an important way for plants to cope with various environmental stresses. This review focuses on identified genes that have known to regulate the induction and initiation of ARs and offers an analysis of this process at the molecular level. The critical genes involved in adventitious rooting are the auxin signaling-responsive genes, including the AUXIN RESPONSE FACTOR (ARF) and the LATERAL ORGAN BOUNDARIES-DOMAIN (LOB) gene families, and genes associated with auxin transport and homeostasis, the quiescent center (QC) maintenance, and the root apical meristem (RAM) initiation. Several genes involved in cell wall modulation are also known to be involved in the regulation of adventitious rooting. Furthermore, the molecular processes that play roles in the ethylene, cytokinin, and jasmonic acid signaling pathways and their crosstalk modulate the generation of ARs. The crosstalk and interaction among many molecular processes generates complex networks that regulate AR generation.

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SAUR15 Promotes Lateral and Adventitious Root Development via Activating H⁺-ATPases and Auxin Biosynthesis

Cited by 42 publications

References 82 publications

WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and indole‐3‐acetic acid‐conjugating genes and by inhibiting ammonium efflux in the root elongation zone

WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and indole‐3‐acetic acid‐conjugating genes and by inhibiting ammonium efflux in the root elongation zone

Identification of transcription factors controlling cell wall invertase gene expression for reproductive development via bioinformatic and transgenic analyses

Molecular Bases for the Regulation of Adventitious Root Generation in Plants

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SAUR15 Promotes Lateral and Adventitious Root Development via Activating H+-ATPases and Auxin Biosynthesis

Cited by 42 publications

References 82 publications

WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and indole‐3‐acetic acid‐conjugating genes and by inhibiting ammonium efflux in the root elongation zone

WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and indole‐3‐acetic acid‐conjugating genes and by inhibiting ammonium efflux in the root elongation zone

Identification of transcription factors controlling cell wall invertase gene expression for reproductive development via bioinformatic and transgenic analyses

Molecular Bases for the Regulation of Adventitious Root Generation in Plants

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SAUR15 Promotes Lateral and Adventitious Root Development via Activating H⁺-ATPases and Auxin Biosynthesis