Sauerstoffdissoziationskurven des Blutes von S�ugetieren

Bartels, H.; Harms, H.

doi:10.1007/bf00380734

Cited by 237 publications

(36 citation statements)

References 24 publications

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“…0, tension and pH were determined by an IL pH/gas analyzer 113 S1 (Instrumentation Laboratories, Boston, MA). Oxygen hemoglobin equilibrium curves were constructed graphically according to the method of Bartels and Harms (1). Their position is expressed by log P,, at pH 7.15 using Bohr factors (Alog PO,/ApH) -0.39 for 2,3-DPG-free hemoglobin solutions, -0.43 at 1 mM 2,3-DPG, and -0.56 at 5 mM 2,3-DPG.…”

Section: Procedures and Methodsmentioning

confidence: 99%

Oxygen Affinity of Hemoglobins F and A Partially Oxidized to Methemoglobin: Influence of 2,3-Diphosphoglycerate

et al. 1978

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Section: Procedures and Methodsmentioning

confidence: 99%

Oxygen Affinity of Hemoglobins F and A Partially Oxidized to Methemoglobin: Influence of 2,3-Diphosphoglycerate

et al. 1978

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“…Their average Wintrobe hematocrit was normal for the guinea pig, i.e., 38.5±4 ml/100 ml blood (11). Oxgen saturation of the blood was calculated from the pH and P02 using the guinea pig 02 dissociation curve (12). From the 02 saturation and the hemoglobin concentration (as derived from the hematocrit) the 02 content of hemoglobin was calculated.…”

Section: Methodsmentioning

confidence: 99%

Role of the Mast Cell in the Pulmonary Pressor Response to Hypoxia

Haas¹,

Bergofsky²

1972

J. Clin. Invest.

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A B S T R A C T This study investigated the role of the mast cell in the pulmonary arterial pressor response to hypoxia. We found that pulmonary arteries 50-500 iA in diameter have a predictable distribution of perivascular mast cells; that such pulmonary mast cells are degranulated in vivo during alveolar hypoxia; that hypoxia releases histamine from mast cells isolated from the peritoneal cavity without apparent injury to the cells; and that histamine is released from the lung of intact guinea pigs during alveolar hypoxia, with the rise in pulmonary vascular resistance during this period proportional to the amount of histamine released. These data point to the perivascular pulmonary mast cell in the rat and guinea pig as an important structure in the mediation of the pulmonary pressor response to hypoxia, even though the responsible humoral vasoconstrictor released from such a cell may not be histamine, or histamine alone. INTRODUCTIONThe long-standing search for the mechanism whereby hypoxia elicits pulmonary vasoconstriction has recently focused on local events at the site of the small pulmonary resistance vessel and its smooth muscle cell (1).

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“…Oxygen saturation was measured by an analyzer for human blood and values were corrected by means of the oxyhemoglobin dissociation curve for pigs [24] . Arterial oxygen delivery (DO 2 ) and oxygen uptake (VO 2 …”

Section: Calculationsmentioning

confidence: 99%

Measurements of Subcutaneous Tissue PO<sub>2</sub> Reflect Oxygen Metabolism of the Small Intestinal Mucosa during Hemorrhage and Resuscitation

et al. 2009

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Background: The first tissues to be deprived of perfusion and oxygenation in a hypovolemic situation and the last ones to be reperfused are the subcutaneous tissue and the gastrointestinal mucosa. We hypothesized that measurements of subcutaneous tissue gases and pH might reflect simultaneous changes in oxygenation of the intestinal mucosa. The aim of this study was to evaluate tissue gases and pH as measures of tissue oxygenation and tissue oxygen metabolism in subcutaneous and intestinal tissues simultaneously. Material and Methods: Five out of 17 domestic pigs (weight 21–25 kg) were used as controls without bleeding. Twelve animals were bled in 3 steps, 10% of their calculated blood volume at each step. The removed blood, crystalloid and colloid were thereafter infused, and animals were stabilized for 30 min. Measurements were made after each step of bleeding, retransfusion and stabilization. Before bleeding, all animals had a sensor (Paratrend 7™) implanted subcutaneously in the left groin for measurements of tissue gases and pH (P_scO₂, P_scCO₂ and pH_sc). Catheters were positioned in the jugular vein, portal vein, carotid artery, pulmonary artery and femoral artery for infusion, bleeding and oxygen monitoring. Via a midline laparotomy, 2 silicon tonometers (TRIP™ sigmoid catheters) were positioned in the ileum and sigmoid colon for measurements of PCO₂ and pH (P_iICO₂, P_siCO₂, pH_iI and pH_si). Blood flow in the portal vein was measured by an ultrasound probe (H6SB) and a Clark electrode (Cardiff tissue oxymeter) was used for serosal PO₂ measurements of the ileum (P_iIO₂) and sigmoid colon (P_siO₂). Results: After the first step of bleeding, P_scO₂ decreased from 64 ± 17 to 56 ± 22 mm Hg (SD; p < 0.05). P_scCO₂ and pH_sc did not change. P_iICO₂ increased from 64 ± 14 to 79 ± 14 mm Hg (p < 0.05), P_siCO₂ increased from 77 ± 16 to 90 ± 18 mm Hg (p < 0.05). pH_iI decreased from 7.15 ± 0.09 to 7.03 ± 0.09 (p < 0.05). P_iIO₂ and P_siO₂ decreased, but not significantly until steps of further bleeding. After re-transfusion and stabilization, P_scO₂ and P_iICO₂ returned to baseline. Conclusion: Measurements of subcutaneous PO₂ are sensitive to bleeding and resuscitation and reflect oxygen metabolism in the small intestinal mucosa as measured by PCO₂ and pH.

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Sauerstoffdissoziationskurven des Blutes von S�ugetieren

Cited by 237 publications

References 24 publications

Oxygen Affinity of Hemoglobins F and A Partially Oxidized to Methemoglobin: Influence of 2,3-Diphosphoglycerate

Oxygen Affinity of Hemoglobins F and A Partially Oxidized to Methemoglobin: Influence of 2,3-Diphosphoglycerate

Role of the Mast Cell in the Pulmonary Pressor Response to Hypoxia

Measurements of Subcutaneous Tissue PO<sub>2</sub> Reflect Oxygen Metabolism of the Small Intestinal Mucosa during Hemorrhage and Resuscitation

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