Saturating the genetic map of <i>Arabidopsis thaliana</i> with embryonic mutations

Franzmann, Linda H.; Yoon, Elizabeth S.; Meinke, David W.

doi:10.1046/j.1365-313x.1995.7020341.x

Cited by 95 publications

(88 citation statements)

References 22 publications

(31 reference statements)

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“…There are several reports of an inserted T-DNA not causing a particular mutant phenotype (42)(43)(44)(45)(46)(47). To determine whether the T-DNA insertion caused the rat5 phenotype, a complemen-tation analysis was performed.…”

Section: Discussionmentioning

confidence: 99%

An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration

Mysore

Nam

Gelvin

2000

Proc. Natl. Acad. Sci. U.S.A.

154

150

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Agrobacterium tumefaciens genetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, the T-DNA, to the plant and integrating the T-DNA into the plant genome. Little is known about the T-DNA integration process, and no plant genes involved in integration have yet been identified. We characterized an Arabidopsis mutant generated by T-DNA insertional mutagenesis, rat5, that is resistant to Agrobacterium root transformation. rat5 contains two copies of T-DNA integrated as a tandem direct repeat into the 3 untranslated region of a histone H2A gene, upstream of the polyadenylation signal sequence. Transient and stable ␤-glucuronidase expression data and assessment of the amount of T-DNA integrated into the genomes of wild-type and rat5 Arabidopsis plants indicated that the rat5 mutant is deficient in T-DNA integration. We complemented the rat5 mutation by expressing the RAT5 histone H2A gene in the mutant plant. Overexpression of RAT5 in wild-type plants increased Agrobacterium transformation efficiency. Furthermore, transient expression of a RAT5 gene from the incoming T-DNA was sufficient to complement the rat5 mutant and to increase the transformation efficiency of wild-type Arabidopsis plants.T-DNA transformation ͉ haplo-insufficiency

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Section: Discussionmentioning

confidence: 99%

An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration

Mysore

Nam

Gelvin

2000

Proc. Natl. Acad. Sci. U.S.A.

154

150

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“…A search of the Arabidopsis mutant database revealed the presence of two known embryo defective mutants, emb34 and erpb93, that map close to this location. The T-QNA insertional mutant emb93 maps at 11 cM on chromosome 2 on the classical map (Castle et al, 1993;Franzmann et al, 1995). Embyro development in emb93 is blocked at the transition from the globular to cotyledon stage of seed development.…”

Section: Discussionmentioning

confidence: 99%

Antisense Expression of the Peptide Transport Gene AtPTR2-B Delays Flowering and Arrests Seed Development in Transgenic Arabidopsis Plants

et al. 1997

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Previously, we identified a peptide transport gene, AfPTRZ-B, from Arabidopsis fhaliana that was constitutively expressed in all plant organs, suggesting an important physiological role in plant growth and development. To evaluate the function of this transporter, transgenic Arabidopsis plants were constructed expressing antisense or sense AfPTRZ-B. Genomic Southern analysis indicated that four independent antisense and three independent sense AtPTRZ-B transgenic lines were obtained, which was confirmed by analysis of the segregation of the kanamycin resistance gene carried on the T-DNA. RNA blot data showed that the endogenous AfPJRZ-B mRNA levels were significantly reduced in transgenic leaves and flowers, but not in transgenic roots. Consistent with this reduction in endogenous AfPTRZ-B mRNA levels, all four antisense lines and one sense line exhibited significant phenotypic changes, including late flowering and arrested seed development. These phenotypic changes could be explained by a defect in nitrogen nutrition due to the reduced peptide transport activity conferred by AtPTR2-B. These results suggest that AtPTRZ-B may play a general role in plant nutrition. l h e AtPTR2-B gene was mapped to chromosome 2, which is closely linked to the restriction fragment length polymorphism marker 111246.

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“…TT1 mRNA was not detectable by RNA gel blot techniques using polyA+ RNA or by in situ localization with radioactively or digoxigeninlabeled riboprobes (E. Schmelzer, unpubl.). However, the TT1 mRNA was detected by RT-PCR in flowers and developing siliques, but not in roots, stems, or young or (Koornneef 1990;Franzmann et al 1995) close to the centromere (black circle). Five BAC clones (black bars; BAC designation on the right) which hybridized to tt1-2ϻEn DNA sequence and which contained marker ms100 mapped between markers agp6e and mi63.…”

Section: Accumulation Pattern Of Tt1 Mrnamentioning

confidence: 99%

A. thaliana TRANSPARENT TESTA 1 is involved in seed coat development and defines the WIP subfamily of plant zinc finger proteins

Sagasser¹,

Lü²,

Hahlbrock³

et al. 2002

Genes Dev.

182

153

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Seeds of the Arabidopsis thaliana transparent testa 1 mutant (tt1) appear yellow, due to the lack of condensed tannin pigments in the seed coat. The TT1 gene was isolated by reverse genetics using an En-1 transposon mutagenized A. thaliana population. TT1 gene expression was detected in developing ovules and young seeds only, and the gene was shown to encode a nuclear protein. Mutant seeds displayed altered morphology of the seed endothelium in which brown tannin pigments accumulate in wild-type plants, indicating that TT1 is involved in the differentiation of this cell layer. When overexpressed in transgenic A. thaliana plants, TT1 caused aberrant development and organ morphology. The protein contains a novel combination of two TFIIIA-type zinc finger motifs. Closely related motifs were detected in a number of putative proteins deduced from plant genomic and EST sequences. The new protein domain containing this type of zinc finger motifs was designated WIP, according to three strictly conserved amino acid residues. Our data indicate the existence of a small gene family in A. thaliana which is defined by the occurrence of the WIP domain. WIP genes may play important roles in regulating developmental processes, including the control of endothelium differentiation.

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Saturating the genetic map of Arabidopsis thaliana with embryonic mutations

Cited by 95 publications

References 22 publications

An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration

An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration

Antisense Expression of the Peptide Transport Gene AtPTR2-B Delays Flowering and Arrests Seed Development in Transgenic Arabidopsis Plants

A. thaliana TRANSPARENT TESTA 1 is involved in seed coat development and defines the WIP subfamily of plant zinc finger proteins

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