Saturated hydrogen alleviates CCl₄-induced acute kidney injury via JAK2/STAT3/p65 signaling

Abstract

This study assessed the protective effects of saturated hydrogen against CCl 4-induced acute kidney injury (AKI) in mice, and investigated signaling pathways activated by exposure to saturated hydrogen. Methods: A mouse model of CCl 4-induced AKI was established; some mice were treated with saturated hydrogen. Levels of cystatin C and kidney injury molecule 1 were determined using enzyme-linked immunosorbent assays. Blood urea nitrogen and serum creatinine were measured on a fully automated biochemical analyzer. Interleukin-8, tumor necrosis factor-a, and interferon-c in serum and kidney tissues were measured using enzyme-linked immunosorbent assays. Malondialdehyde, glutathione peroxidase, and superoxide dismutase in kidney tissues were measured using biochemical kits. Oxidative stress in kidney tissues was analyzed using nitrotyrosine staining. Expression levels of p-JAK2, p-STAT3, and p-p65 signal protein were assayed by immunohistochemistry and western blotting. Results: Compared with untreated mice with CCl 4-induced AKI, mice that were treated with saturated hydrogen exhibited improved renal function and reduced oxidative stress. Moreover, expression levels of p-JAK2, p-STAT3, and p-p65 were significantly reduced in mice treated with saturated hydrogen, compared with expression levels in untreated mice. Conclusions: Treatment with saturated hydrogen can reduce oxidative stress and inflammatory cytokine activation, potentially through inhibition of JAK2/STAT3/p65 signaling, thereby protecting against AKI.

Loganin exerts a protective effect on ischemia–reperfusion‐induced acute kidney injury by regulating JAK2/STAT3 and Nrf2/HO‐1 signaling pathways

“…Zhu et al (Zhu et al, 2020)reported that curcumin alleviated septic AKI via inhibiting JAK2/STAT3 signaling pathway. Wu and his colleagues revealed that saturated hydrogen attenuated AKI by repressing JAK2/STAT3 signaling pathway (Wu et al, 2020). Moreover, diosmetin relieved AKI through enhancing Nrf2/HO‐1 signaling pathway (Wang, Zhang, et al, 2020), sirtuin 5 reduced AKI by promoting Nrf2/HO‐1 signaling pathway (Li et al, 2019).…”

Hydrogen: A Novel Option in Human Disease Treatment

“…HRS markedly reduced interstitial congestion, edema, and hemorrhage in renal tissue, prevented renal injury, and promoted renal function recovery after I/R injury in rats through antiapoptotic and anti-inflammatory actions in kidney cells [ 146 ]. Other authors described that HW significantly reduced the increased postvoid residual volume in obstructed rats and ameliorated bladder dysfunction secondary to bladder outlet obstruction by attenuating oxidative stress [ 147 ].…”

The Inhibitory Effect of Geraniol on CCL4-induced Hepatorenal Toxicity in Pregnant Mice through the PI3K/AKT Signaling Pathway

“…In addition, histological studies on the kidney of CCL 4 -treated mice were similar with other studies. [ 51 52 53 ]…”