Saturated and <i>cis/trans</i> Unsaturated Acyl CoA Esters Differentially Regulate Wild-Type and Polymorphic β-Cell ATP-Sensitive K+ Channels

Riedel, Michael; Light, Peter E.

doi:10.2337/diabetes.54.7.2070

Cited by 39 publications

(40 citation statements)

References 42 publications

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“…Fatty acids are key components of cellular membrane phospholipids and are involved in intracellular mechanisms, as previously described [12,13]. Previous studies reported the stimulatory effect of the monounsaturated palmitoleic acid (C16:1) on KATP channel activity [16] and promotion of β-cell function and proliferation at low glucose concentrations [27]. Although we observed a significant increase in palmitoleic acid (C16:1) in untreated diabetic islets (Fig.…”

Section: Discussionsupporting

confidence: 77%

“…Thus, it is clear that changes in the contents of fatty acids can influence membrane structure and function of islet cells, namely the activities of β-cell channels and receptors such as K + , Ca 2+ and voltage-sensitive channels, glucose transporters, and adenylate cyclasecoupled and phospholipase C-coupled receptors [14]. Furthermore, phospholipids, diacylglycerol, phosphatidic acid and triacylglycerol are implicated in different cellular mechanisms, as they can be precursors or generators of important molecules such as prostaglandins and ceramides and they are involved also in gene expression, influencing a range of glucose and lipid signalling pathways [8,10,[13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Accumulating evidence suggests that chain length, spatial conformation and degree of saturation of FFAs profoundly influence the insulinotropic potency and the stimulation of KATP channel activity [7,16,[18][19][20]. Studies focussed on fatty acids chain length reported that the increase of the number of carbons has a negative effect on insulin release by increasing β-cell KATP channel activity and induces uncoupling of β-cell oxidative phosphorylation [7,16,18].…”

Section: Introductionmentioning

confidence: 99%

“…Studies focussed on fatty acids chain length reported that the increase of the number of carbons has a negative effect on insulin release by increasing β-cell KATP channel activity and induces uncoupling of β-cell oxidative phosphorylation [7,16,18]. Concerning the spatial conformation, trans fatty acids induce better results in insulin release than the corresponding cis isoforms [21].…”

Section: Introductionmentioning

confidence: 99%

“…Concerning the spatial conformation, trans fatty acids induce better results in insulin release than the corresponding cis isoforms [21]. However, it has been shown that cis monounsaturated fatty acids (MUFAs) have a positive effect on glycaemic control [16,22].…”

Section: Introductionmentioning

confidence: 99%

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Soybean oil treatment impairs glucose-stimulated insulin secretion and changes fatty acid composition of normal and diabetic islets

et al. 2007

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We investigated the effect of sub-chronic soybean oil (SO) treatment on the insulin secretion and fatty acid composition of islets of Langerhans obtained from Goto-Kakizaki (GK), a model of type 2 diabetes, and normal Wistar rats. We observed that soybean-treated Wistar rats present insulin resistance and defective islet insulin secretion when compared with untreated Wistar rats. The decrease in insulin secretion occurred at all concentrations of glucose and arginine tested. Furthermore we observed that soybean-treated normal islets present a significant decrease in two saturated fatty acids, myristic and heneicosanoic acids, and one monounsaturated eicosenoic acid, and the appearance of the monounsaturated erucic acid. Concerning diabetic animals, we observed that soybean-treated diabetic rats, when compared with untreated GK rats, present an increase in plasma non-fasting free fatty acids, an exacerbation of islet insulin secretion impairment in all conditions tested and a significant decrease in the monounsaturated palmitoleic acid. Altogether our results show that SO treatment results in a decrease of insulin secretion and alterations on fatty acid composition in normal and diabetic islets. Furthermore, the impairment of insulin secretion, islet erucic acid and fasting plasma insulin levels are similar in treated normal and untreated diabetic rats, suggesting that SO could have a deleterious effect on β-cell function and insulin sensitivity.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Soybean oil treatment impairs glucose-stimulated insulin secretion and changes fatty acid composition of normal and diabetic islets

et al. 2007

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Current literature in diabetes

2006

Diabetes Metabolism Res

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of diabetes/metabolism. Each bibliography is divided into 26 sections: 1 Reviews & Symposia; 2 General; 3 Genetics; 4 Epidemiology; 5 Immunology; 6 Obesity; 7 Prediction and Prevention; 8 Intervention: a) General; b) Care; c) Drug Therapy; d)Economics; e) Gene therapy; f) Nursing; g) Nutrition; h) Surgery; i) Transplantation; 9 Pathology and Complications: a) General; b) Cardiovascular; c) Eye disease; d) Gestational and fetal; e) Neurological; f) Podiatrical; g) Renal; 10 Endocrinology & Metabolism; 11 Experimental Studies; 12 Diagnosis and Techniques. Within each section, articles are listed in alphabetical order with respect to author

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Glucose‐induced de novo synthesis of fatty acyls causes proportional increases in INS‐1E cellular lipids

et al. 2007

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Raised concentrations of glucose for extended periods of time have detrimental effects on the insulin-producing beta-cell. As de novo synthesis of lipids has been observed under such conditions, it was hypothesized that newly formed lipids may preferentially contain saturated fatty acids, which in particular have been associated with impaired beta-cell function. Glucose-induced de novo synthesis of fatty acids in INS-1E cells cultured in 5.5, 11, 20 or 27 mM glucose for 5 days was assessed by high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy and gas chromatography-mass spectrometry (GC-MS). The glucose origin of the increase in fatty acyls was verified by replacing glucose with [1-13C]glucose during culture followed by analysis with two-dimensional 1H-13C NMR spectroscopy. The composition of the fatty acyls was determined by GC-MS. Fatty acyls determined by HR-MAS (1)H NMR spectroscopy were increased fivefold in INS-1E cells cultured in 20 or 27 mM glucose compared with cells cultured in 5.5 mM glucose. The five most abundant fatty acids with their relative percentages in INS-1E cells cultured in 5.5 mM glucose were oleate (33%), palmitate (25%), stearate (19%), octadecenoate (13%) and palmitoleate (4.4%). These proportions were not affected by glucose- induced de novo synthesis in INS-1E cells cultured in 11, 20 or 27 mM glucose. It is concluded that glucose-induced de novo lipid synthesis results in accumulation of both saturated and unsaturated fatty acids in specific proportions that are identical with those present under control conditions.

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Saturated and cis/trans Unsaturated Acyl CoA Esters Differentially Regulate Wild-Type and Polymorphic β-Cell ATP-Sensitive K+ Channels

Cited by 39 publications

References 42 publications

Soybean oil treatment impairs glucose-stimulated insulin secretion and changes fatty acid composition of normal and diabetic islets

Soybean oil treatment impairs glucose-stimulated insulin secretion and changes fatty acid composition of normal and diabetic islets

Current literature in diabetes

Glucose‐induced de novo synthesis of fatty acyls causes proportional increases in INS‐1E cellular lipids

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