Satisfying the need for rapid diagnosis of new variant influenza A H1N1

Vries, Erhard van der; Schutten, Martin

doi:10.1586/erm.10.18

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…RT-qPCR has proven to be sensitive and a good alternative for conventional diagnostic methods. 22 However, comparing quantitative influenza RT-PCR data between laboratories and studies is hampered by the lack of an international accepted standard. 23 In the present study, we made use of two commercially available electron microscopyecounted influenza virus stocks for calculation of a viral particle count, instead of in-house quantified RNA or armed particle stocks.…”

Section: Discussionmentioning

confidence: 99%

Molecular Assays for Quantitative and Qualitative Detection of Influenza Virus and Oseltamivir Resistance Mutations

Vries

Anber

Linden

et al. 2013

The Journal of Molecular Diagnostics

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Sensitive and reproducible molecular assays are essential for influenza virus diagnostics. This manuscript describes the design, validation, and evaluation of a set of real-time RT-PCR assays for quantification and subtyping of human influenza viruses from patient respiratory material. Four assays are included for detection of oseltamivir resistance mutations H275Y in prepandemic and pandemic influenza A/H1N1 and E119V and R292K in influenza A/H3N2 neuraminidase. The lower limits of detection of the quantification assay were determined to be 1.7 log(10) virus particles per milliliter (vp/mL) for influenza A and 2.2 log(10) vp/mL for influenza B virus. The lower limits of quantification were 2.1 and 2.3 log(10) vp/mL, respectively. The RT-PCR efficiencies and lower limits of detection of the quantification assays were only marginally affected when tested on the most dissimilar target sequences found in the GenBank database. Finally, the resistance RT-PCR assays detected at least 5% mutant viruses present in mixtures containing both wild-type and mutant viruses with approximated limits of detection of 2.4 log(10) vp/mL. Overall, this set of RT-PCR assays is a powerful tool for enhanced influenza virus surveillance.

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Section: Discussionmentioning

confidence: 99%

Molecular Assays for Quantitative and Qualitative Detection of Influenza Virus and Oseltamivir Resistance Mutations

Vries

Anber

Linden

et al. 2013

The Journal of Molecular Diagnostics

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“…Rapid diagnosis of influenza is important for introduction of antiviral therapy and quarantine measures, since antiviral therapy should preferably be initiated within 24 h after appearance of the patient's first clinical symptoms (12). This article describes a nucleic acid lateral-flow (NALF) assay, called the rapidSTRIPE assay, used as a molecular-genetic rapid test for the diagnosis of the pandemic S-OIV A (H1N1) virus.…”

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confidence: 99%

rapidSTRIPE H1N1 Test for Detection of the Pandemic Swine Origin Influenza A (H1N1) Virus

Patel¹,

Graser²,

Robst³

et al. 2011

J Clin Microbiol

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Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay

Cho²,

Cho³

et al. 2013

Osong Public Health and Research Perspectives

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ObjectivesThe aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.Materials and methodsSeveral monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR).ResultsThe detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005).ConclusionThese results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.

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Satisfying the need for rapid diagnosis of new variant influenza A H1N1

Cited by 3 publications

References 7 publications

Molecular Assays for Quantitative and Qualitative Detection of Influenza Virus and Oseltamivir Resistance Mutations

Molecular Assays for Quantitative and Qualitative Detection of Influenza Virus and Oseltamivir Resistance Mutations

rapidSTRIPE H1N1 Test for Detection of the Pandemic Swine Origin Influenza A (H1N1) Virus

Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay

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