Satellite Glial Cells of the Dorsal Root Ganglion: A New “Guest/Physiopathological Target” in ALS

Ruiz-Soto, María; Riancho, Javier; Tapia, Olga; Lafarga, Miguel; Berciano, Marı́a T.

doi:10.3389/fnagi.2020.595751

Cited by 10 publications

(16 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, in TS GCs, we assessed the detection of peroxidized lipids, a cellular indicator of ROS imbalance and of oxidative damage to lipids [ 59 , 60 ]. For this purpose, we used an in situ assay with Bodipy C11, a lipid-soluble ratiometric fluorescent indicator for the peroxidation of certain lipids of membranous organelles [ 61 ]. We found that the cytoplasmic fluorescent signal of Bodipy C11 increased significantly in TS GCs as compared to those of controls ( Figure 1 H–J).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative-Stress-Associated Proteostasis Disturbances and Increased DNA Damage in the Hippocampal Granule Cells of the Ts65Dn Model of Down Syndrome

et al. 2022

Self Cite

View full text Add to dashboard Cite

Oxidative stress (OS) is one of the neuropathological mechanisms responsible for the deficits in cognition and neuronal function in Down syndrome (DS). The Ts65Dn (TS) mouse replicates multiple DS phenotypes including hippocampal-dependent learning and memory deficits and similar brain oxidative status. To better understand the hippocampal oxidative profile in the adult TS mouse, we analyzed cellular OS-associated alterations in hippocampal granule cells (GCs), a neuronal population that plays an important role in memory formation and that is particularly affected in DS. For this purpose, we used biochemical, molecular, immunohistochemical, and electron microscopy techniques. Our results indicate that TS GCs show important OS-associated alterations in the systems essential for neuronal homeostasis: DNA damage response and proteostasis, particularly of the proteasome and lysosomal system. Specifically, TS GCs showed: (i) increased DNA damage, (ii) reorganization of nuclear proteolytic factories accompanied by a decline in proteasome activity and cytoplasmic aggregation of ubiquitinated proteins, (iii) formation of lysosomal-related structures containing lipid droplets of cytotoxic peroxidation products, and (iv) mitochondrial ultrastructural defects. These alterations could be implicated in enhanced cellular senescence, accelerated aging and neurodegeneration, and the early development of Alzheimer’s disease neuropathology present in TS mice and the DS population.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Moreover, some complex bodies with several lipid droplets may be lipofuscin residual granules containing indigestible oxidized lipids and proteins. Indeed, some cytoplasmic bodies in TS GCs displayed a strong autofluorescence with confocal microscopy ( Figure 6 F), a typical characteristic of lipofuscin granules [ 61 , 86 ].…”

Section: Resultsmentioning

confidence: 99%

Oxidative-Stress-Associated Proteostasis Disturbances and Increased DNA Damage in the Hippocampal Granule Cells of the Ts65Dn Model of Down Syndrome

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…During this period, it was unclear whether satellite cells were interconnected, if they completely sheath neuronal somata, or if pores that bypassed the satellite layer allowed direct communication between neuron and interstitial space. Several subsequent investigations have shown that individual DRG neurons of different species were normally completely surrounded by satellite cells (Cervos-Navarro 1959 ; Pannese 1960 ; Rigon et al 2013 ; Ruiz-Soto et al 2020 ). Scanning EM analysis showed that the neuron-satellite complexes in frogs and chickens have diameters of about 25 μm respectively (Matsuda et al 2005 ).…”

Section: Structure Of Satellite Cellsmentioning

confidence: 99%

“…Satellite cells in DRG of mice have round-ovoid nuclei with a diameter of 4–7 μm, a double layer nuclear membrane, and a homogenous nucleoplasm with a peripheral enrichment of heterochromatin (Table 2 ) (Ruiz-Soto et al 2020 ). The chromatin of the satellite cell nucleus appears to be denser compared to neuronal chromatin.…”

Section: Organelles In Satellite Cellsmentioning

confidence: 99%

Ultrastructure of dorsal root ganglia

et al. 2023

View full text Add to dashboard Cite

Dorsal root ganglia (DRG) contains thousands of sensory neurons that transmit information about our external and internal environment to the central nervous system. This includes signals related to proprioception, temperature, and nociception. Our understanding of DRG has increased tremendously over the last 50 years and has established the DRG as an active participant in peripheral processes. This includes interactions between neurons and non-neuronal cells such as satellite glia cells and macrophages that contribute to an increasingly complex cellular environment that modulates neuronal function. Early ultrastructural investigations of the DRG have described subtypes of sensory neurons based on differences in the arrangement of organelles such as the Golgi apparatus and the endoplasmic reticulum. The neuron-satellite cell complex and the composition of the axon hillock in DRG have also been investigated, but, apart from basic descriptions of Schwann cells, ultrastructural investigations of other cell types in DRG are limited. Furthermore, detailed descriptions of key components of DRG, such as blood vessels and the capsule that sits at the intersection of the meninges and the connective tissue covering the peripheral nervous system, are lacking to date. With rising interest in DRG as potential therapeutic targets for aberrant signalling associated with chronic pain conditions, gaining further insights into DRG ultrastructure will be fundamental to understanding cell–cell interactions that modulate DRG function. In this review, we aim to provide a synopsis of the current state of knowledge on the ultrastructure of the DRG and its components, as well as to identify areas of interest for future studies.

show abstract

“…In the nervous system, glial cells including microglia, astrocytes and Schwann cells (SCs) etc., could support and guide neuronal migration, regeneration and immune response via differentiation and proliferation [ 25 ]. SCs are the main glial cells in the peripheral nervous system, which are wrapped in the outer layer of axons and play a key role in the regeneration and repair process of peripheral nerve injury [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration

Zheng

Sun

et al. 2022

Burns &Amp; Trauma

View full text Add to dashboard Cite

Background Anisotropic topologies are known to regulate cell-oriented growth and induce cell differentiation, which is conducive to accelerating nerve regeneration, while co-culture of endothelial cells (ECs) and Schwann cells (SCs) can significantly promote the axon growth of dorsal root ganglion (DRG). However, the synergistic regulation of EC and SC co-culture of DRG behavior on anisotropic topologies is still rarely reported. The study aims to investigate the effect of anisotropic topology co-cultured with Schwann cells and endothelial cells on dorsal root ganglion behavior for promoting peripheral nerve regeneration. Methods Chitosan/artemisia sphaerocephala (CS/AS) scaffolds with anisotropic topology were first prepared using micro-molding technology, and then the surface was modified with dopamine to facilitate cell adhesion and growth. The physical and chemical properties of the scaffolds were characterized through morphology, wettability, surface roughness and component variation. SCs and ECs were co-cultured with DRG cells on anisotropic topology scaffolds to evaluate the axon growth behavior. Results Dopamine-modified topological CS/AS scaffolds had good hydrophilicity and provided an appropriate environment for cell growth. Cellular immunofluorescence showed that in contrast to DRG growth alone, co-culture of SCs and ECs could not only promote the growth of DRG axons, but also offered a stronger guidance for orientation growth of neurons, which could effectively prevent axons from tangling and knotting, and thus may significantly inhibit neurofibroma formation. Moreover, the co-culture of SCs and ECs could promote the release of nerve growth factor and vascular endothelial growth factor, and up-regulate genes relevant to cell proliferation, myelination and skeletal development via the PI3K-Akt, MAPK and cytokine and receptor chemokine pathways. Conclusions The co-culture of SCs and ECs significantly improved the growth behavior of DRG on anisotropic topological scaffolds, which may provide an important basis for the development of nerve grafts in peripheral nerve regeneration.

show abstract

Satellite Glial Cells of the Dorsal Root Ganglion: A New “Guest/Physiopathological Target” in ALS

Cited by 10 publications

References 52 publications

Oxidative-Stress-Associated Proteostasis Disturbances and Increased DNA Damage in the Hippocampal Granule Cells of the Ts65Dn Model of Down Syndrome

Oxidative-Stress-Associated Proteostasis Disturbances and Increased DNA Damage in the Hippocampal Granule Cells of the Ts65Dn Model of Down Syndrome

Ultrastructure of dorsal root ganglia

Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration

Contact Info

Product

Resources

About