Sas Is Amplified Predominantly in Surface Osteosarcoma

Abstract

Summary:The development of several types of human tumors is related to amplification of genes that are involved in cell growth. The protein products of these genes give the cells a selective growth advantage. The q13-15 region of chromosome 12 is frequently altered in human sarcomas, and the SAS gene has been identified in an amplification unit mapping to this region. Gene amplification of SAS was analyzed to determine the frequency of genetic alteration of this gene in osteosarcoma. Using Southern blot analysis as well as quantitative polymerase chain reaction, SAS was found to be amplified in 10 (36%) of 28 osteosarcomas. Gene amplification was evaluated in subtypes of osteosarcoma. All seven surface osteosarcomas displayed amplified SAS. In contrast, SAS was amplified in only two (13%) of 15 intramedullary osteosarcomas. The finding that all surface osteosarcomas demonstrated SAS gene amplification suggests that this gene may play a role in the pathogenesis of osteosarcoma subtypes and that surface osteosarcoma may be genetically different from high-grade intramedullary osteosarcoma.-

Immunohistochemical analysis of MDM2 and CDK4 distinguishes low-grade osteosarcoma from benign mimics

“…11,[15][16][17][18][19][20] These results suggest that the similarity between parosteal and low-grade central osteosarcomas stems from the genetic level, further strengthening the relationship between the two tumor subtypes. 1,14 Diagnosis of low-grade osteosarcoma is challenging.…”

Cytogenetics and Molecular Biology of Osteosarcoma

“…The SAS gene, located on chromosome 12q13 along with CDK4 and MDM2, was reported to be amplified in 36% of osteosarcomas and 100% of parosteal osteosarcomas (Noble-Topham et al, 1996). SAS encodes a transmembrane protein believed to be involved in cellular growth processes (Ladanyi and Gorlick, 2000).…”

CDK4 gene amplification in osteosarcoma: Reciprocal relationship withINK4A gene alterations and mapping of 12q13 amplicons

“…4). Two of the 6 tumors which showed amplification were predominantly surface osteosarcomas (parosteal osteosarcoma), a type of sarcoma which may show a higher prevalence of 12q13 amplification (Noble-Topham et al, 1996;Szymanska et al, 1996).…”