SARS-CoV-2 viral RNA detection using the novel CoVradar device associated with the CoVreader smartphone app

Martín-Sierra, Carmen; Tabraue-Chávez, Mavys; Escobedo, Pablo; García-Cabrera, Victor; Lopez‐Delgado, Francisco Javier; Guardia-Monteagudo, Juan J.; Ruiz-García, Isidoro; Erenas, Miguel M.; Sánchez‐Martín, Rosario M.; Capitán-Vallvey, L.F.; Palma, A.; Pernagallo, Salvatore; Díaz‐Mochón, Juan José

doi:10.1016/j.bios.2023.115268

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…On the other hand, the salivary proteome consists of about 2,000 characteristic proteins and peptides that can be used to diagnose a multitude of pathologies [ 6 ]. Furthermore, saliva has been employed as a detection medium for ultrasensitive in-situ immunoassay, serving as a diagnostic tool for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) [ 7 , 8 ] and other viruses, such as human papillomavirus (HPV) [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

POC device for rapid oral pH determination based on a smartphone platform

Arroyo,

Escobedo,

Ruiz-García

et al. 2024

Microchim Acta

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Salivary pH serves as a valuable and useful diagnostic marker for periodontal disease, as it not only plays a critical role in disease prevention but also in its development. Typically, saliva sampling is collected by draining and spitting it into collection tubes or using swabs. In this study, we have developed a Point-of-Care (POC) device for in situ determination of oral pH without the need for complex instruments, relying solely on a smartphone as the detection device. Our system utilizes a non-toxic vegetable colourimetric indicator, immobilized on a chitosan membrane located on a disposable stick, enabling direct sampling within the buccal cavity. An ad hoc designed 3D-printed attachment is used to ensure accurate positioning and alignment of the stick, as well as isolation from external lighting conditions. A custom-developed smartphone application captures and automatically processes the image of the sensing membrane, providing the salivary pH results. After optimizing the cocktail composition, the developed sensors demonstrated the capacity to determine pH within a range of 5.4 to 8.1 with a remarkable precision of 0.6%, achieving a very short analysis time of just 1 min. A stability study conducted on the sensing membranes revealed a lifetime of 50 days. To validate the performance of our analytical device, we compared its results against those obtained from a calibrated pH-meter, using a group of individuals. The device exhibited an average error of 2.4% when compared with the pH-meter results, confirming its reliability and accuracy. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

POC device for rapid oral pH determination based on a smartphone platform

Arroyo,

Escobedo,

Ruiz-García

et al. 2024

Microchim Acta

Self Cite

View full text Add to dashboard Cite

show abstract

“…18,20–22,24–32 In addition, DCL has been applied to detect a variety of pathogens, including viruses and parasites, and can also analyse alpha satellite DNA in in situ hybridization. 33–36…”

Section: Introductionmentioning

confidence: 99%