SARS-CoV-2 seroepidemiological study in healthcare workers and discordant results using seven different diagnostic methods

Tatsi, Elizabeth‐Barbara; Dellis, Charilaos; Petridou, Evangelia; Banou, Kirkira; Zachariadou, Levantia; Syriopoulou, Vassiliki; Michos, Athanasios

doi:10.1007/s15010-021-01653-2

Cited by 3 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seroprevalence in Greece evolved over time and varied across geographic regions and between metropolitan/non-metropolitan areas, with regional units encompassing the cities of Athens and Thessaloniki showing an approximate two-fold increase in seropositivity. Our present work extends from our previously published seroprevalence survey reports, and is in accordance with the limited number of published studies from Greece in high-risk populations, specifically considering health care workers, transmission hot spots or other unique groups such as blood donors [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. These studies all addressed earlier stages of the COVID-19 pandemic.…”

Section: Discussionsupporting

confidence: 90%

SARS-CoV-2 Sero-Surveillance in Greece: Evolution over Time and Epidemiological Attributes during the Pre-Vaccination Pandemic Era

et al. 2022

View full text Add to dashboard Cite

Background: Nation-wide SARS-CoV-2 seroprevalence surveys provide valuable insights into the course of the pandemic, including information often not captured by routine surveillance of reported cases. Methods: A serosurvey of IgG antibodies against SARS-CoV-2 was conducted in Greece between March and December 2020. It was designed as a cross-sectional survey repeated at monthly intervals. The leftover sampling methodology was used and a geographically stratified sampling plan was applied. Results: Of 55,947 serum samples collected, 705 (1.26%) were found positive for anti-SARS-CoV-2 antibodies, with higher seroprevalence (9.09%) observed in December 2020. Highest seropositivity levels were observed in the “0–29” and “30–49” year age groups. Seroprevalence increased with age in the “0–29” age group. Highly populated metropolitan areas were characterized with elevated seroprevalence levels (11.92% in Attica, 12.76% in Thessaloniki) compared to the rest of the country (5.90%). The infection fatality rate (IFR) was estimated at 0.451% (95% CI: 0.382–0.549%) using aggregate data until December 2020, and the ratio of actual to reported cases was 9.59 (7.88–11.33). Conclusions: The evolution of seroprevalence estimates aligned with the course of the pandemic and varied widely by region and age group. Young and middle-aged adults appeared to be drivers of the pandemic during a severe epidemic wave under strict policy measures.

show abstract

Section: Discussionsupporting

confidence: 90%

SARS-CoV-2 Sero-Surveillance in Greece: Evolution over Time and Epidemiological Attributes during the Pre-Vaccination Pandemic Era

et al. 2022

View full text Add to dashboard Cite

show abstract

“…There have been several studies examining the prevalence of SARS-CoV-2 and the seroprevalence of IgM/IgG antibodies in HCWs compared to community levels, and results are varied [ 4 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. Though there have been fewer studies of pediatric HCWs to date, rates of positive serology in pediatric HCWs have consistently been found to be lower or equal to those measured in the general population, with the reported range of positive serology ranging from 0–16.9% [ 5 , 6 , 7 , 8 , 9 , 19 , 20 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although the potential for increased exposure to SARS-CoV-2 among healthcare workers (HCWs) has led to numerous serologic studies, the pediatric HCW population remains somewhat less well surveyed [ 4 , 5 , 6 , 7 , 8 , 9 ]. Pediatric HCW are in general exposed to a large variety of infectious diseases, and during the COVID-19 pandemic, at increased risk for both known and unknown exposure to SARS-CoV-2 given the varied presentation of COVID-19 in children.…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 Serology Testing in an Asymptomatic, At-Risk Population: Methods, Results, Pitfalls

Heyming

Bacon

Lara

et al. 2021

Infectious Disease Reports

View full text Add to dashboard Cite

The primary aim of this study was to determine the seroprevalence of SARS-CoV-2 antibodies in a population of pediatric healthcare workers (HCWs). This study was conducted 14 May–13 July 2020. Study participants included pediatric HCWs at a pediatric hospital with either direct patient contact or close proximity to patient-care areas. SARS-CoV-2 antibodies were assessed via the Wytcote Superbio SARS-CoV-2 IgM/IgG Antibody Fast Detection Kit and the Abbott Architect SARS-CoV-2 IgG assay. Participants underwent RT-PCR testing upon entry to the study and following rapid IgM+/IgG+ results; respiratory panel PCR (RP-PCR) was performed following IgM+ results. A total of 57 of 289 (19.7%) of participants demonstrated positive serology as assessed by the Wytcote rapid kit (12 on Day 1 and 45 throughout the study). However, only one of these participants demonstrated IgG+ serology via the Abbott assay. Two participants tested SARS-CoV-2+ via RT-PCR testing. One individual was adenovirus+ and enterovirus/rhinovirus+. In our study population, we observed a seroprevalence of SARS-CoV-2 antibodies of 0.35%. The lack of concordance between antibody tests suggests that the Wytcote rapid test kit may not be of use as a screening tool. However, the feasibility of the overall process indicates that a similar methodology may have potential for future epidemiologic surveillance.

show abstract

Socioeconomic and Demographic Risk Factors for SARS-CoV-2 Seropositivity Among Healthcare Workers in a UK Hospital: A Prospective Cohort Study

Lam,

Saso,

Torres Ortiz

et al. 2023

Clinical Infectious Diseases

View full text Add to dashboard Cite

Background To protect healthcare workers (HCWs) from the consequences of disease due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is necessary to understand the risk factors that drive exposure and infection within hospitals. Insufficient consideration of key socioeconomic variables is a limitation of existing studies that can lead to bias and residual confounding of proposed risk factors for infection. Methods The Co-STARs study prospectively enrolled 3679 HCWs between April 2020 and September 2020. We used multivariate logistic regression to comprehensively characterize the demographic, occupational, socioeconomic, and environmental risk factors for SARS-CoV-2 seropositivity. Results After adjusting for key confounders, relative household overcrowding (odds ratio [OR], 1.4 [95% confidence interval {CI}, 1.1–1.9]; P = .006), Black, Black British, Caribbean, or African ethnicity (OR, 1.7 [95% CI, 1.2–2.3]; P = .003), increasing age (ages 50–60 years: OR, 1.8 [95% CI, 1.3–2.4]; P < .001), lack of access to sick pay (OR, 1.8 [95% CI, 1.3–2.4]; P < .001). Conclusions Socioeconomic and demographic factors outside the hospital were the main drivers of infection and exposure to SARS-CoV-2 during the first wave of the pandemic in an urban pediatric referral hospital. Overcrowding and out-of-hospital SARS-CoV-2 contact are less amenable to intervention. However, lack of access to sick pay among externally contracted staff is more easily rectifiable. Our findings suggest that providing easier access to sick pay would lead to a decrease in SARS-CoV-2 transmission and potentially that of other infectious diseases in hospital settings. Clinical Trials Registration NCT04380896.

show abstract

SARS-CoV-2 seroepidemiological study in healthcare workers and discordant results using seven different diagnostic methods

Cited by 3 publications

References 29 publications

SARS-CoV-2 Sero-Surveillance in Greece: Evolution over Time and Epidemiological Attributes during the Pre-Vaccination Pandemic Era

SARS-CoV-2 Sero-Surveillance in Greece: Evolution over Time and Epidemiological Attributes during the Pre-Vaccination Pandemic Era

SARS-CoV-2 Serology Testing in an Asymptomatic, At-Risk Population: Methods, Results, Pitfalls

Socioeconomic and Demographic Risk Factors for SARS-CoV-2 Seropositivity Among Healthcare Workers in a UK Hospital: A Prospective Cohort Study

Contact Info

Product

Resources

About