SARS‐CoV‐2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup

Stadlbauer, Daniel; Amanat, Fatima; Chromikova, Veronika; Jiang, Kaijun; Strohmeier, Shirin; Arunkumar, Guha Asthagiri; Tan, Jessica; Bhavsar, Disha; Capuano, Christina; Kirkpatrick, Ericka; Meade, Philip; Brito, Ruhi Nichalle; Teo, Su Hui Catherine; McMahon, Meagan; Simon, Viviana; Krammer, Florian

doi:10.1002/cpmc.100

Cited by 729 publications

(921 citation statements)

References 13 publications

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“…15 Antibodies to RBD correlate with neutralizing antibodies 16,17 that could be associated with protection based on studies of other coronaviruses and animal models. [17][18][19][20] The RBD antigen, kindly donated by the Krammer lab (Mount Sinai, New York) 21 , was coupled to magnetic MAGPLEX 6.5 μ m COOH-microspheres from Luminex Corporation (Austin, TX) at a concentration of 40 µg/ml for 10,000 beads/µl, as previously pandemic, at 1/500), were added per plate. Two blank control wells with beads in PBS-BN were set up to measure background signal.…”

Section: Quantification Of Antibodies To Sars-cov-2 By Luminexmentioning

confidence: 99%

Seroprevalence of antibodies against SARS-CoV-2 among health care workers in a large Spanish reference hospital

García‐Basteiro

Moncunill

Tortajada

et al. 2020

Preprint

125

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Background: Health care workers (HCW) are a high-risk population to acquire SARS-CoV-2 infection from patients or other fellow HCW. At the same time, they can be contagious to highly vulnerable individuals seeking health care. This study aims at estimating the seroprevalence of antibodies against SARS-CoV-2 and associated factors in HCW from a large referral hospital in Barcelona, Spain, one of the countries hardest hit by COVID-19 in the world. Methods: From 28 March to 9 April 2020, we recruited a random sample of 578 HCW from the human resources database of Hospital Clinic in Barcelona. We collected a nasopharyngeal swab for direct SARS-CoV-2 detection through real time reverse-transcriptase polymerase chain reaction (rRT-PCR), as well as blood for plasma antibody quantification. IgM, IgG and IgA antibodies to the receptor-binding domain of the spike protein were measured by Luminex. The cumulative prevalence of infection (past or current) was defined by a positive SARS-CoV-2 rRT-PCR and/or antibody seropositivity. Results: Of the 578 total participants, 39 (6.7%, 95% CI: 4.8-9.1) had been previously diagnosed with COVID-19 by rRT-PCR, 14 (2.4%, 95% CI: 1.4-4.3) had a positive rRT-PCR at recruitment, and 54 (9.3%, 95% CI: 7.2-12.0) were seropositive for IgM and/or IgG and/or IgA against SARS-CoV-2. Of the 54 seropositive HCW, 21 (38.9%) had not been previously diagnosed with COVID-19, although 10 of them (47.6%) reported past COVID-19-compatible symptoms. The cumulative prevalence of SARS-CoV-2 infection was 11.2% (65/578, 95% CI: 8.9-14.1). Among those with evidence of past or current infection, 40.0% (26/65) had not been previously diagnosed with COVID-19, of which 46.2% (12/26) had history of COVID-19-compatible symptoms. The odds of being seropositive was higher in participants who reported any COVID-19 symptom (OR: 8.84, 95% CI: 4.41-17.73). IgM levels positively correlated with age (rho=0.36, p-value=0.031) and were higher in participants with more than 10 days since onset of symptoms (p-value=0.022), and IgA levels were higher in symptomatic than asymptomatic subjects (p-value=0.041). Conclusions: The seroprevalence of antibodies against SARS-CoV-2 among HCW was lower than expected. Thus, being a high-risk population, we anticipate these estimates to be an upper limit to the seroprevalence of the general population. Forty per cent of those with past or present infection had not been previously diagnosed with COVID-19, which calls for active periodic rRT-PCR testing among all HCW to minimize potential risk of hospital-acquired SARS-CoV-2 infections.

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Section: Quantification Of Antibodies To Sars-cov-2 By Luminexmentioning

confidence: 99%

Seroprevalence of antibodies against SARS-CoV-2 among health care workers in a large Spanish reference hospital

García‐Basteiro

Moncunill

Tortajada

et al. 2020

Preprint

125

View full text Add to dashboard Cite

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“…Along these lines, immune patients will have a low risk of pregnancy complications in the event of an embryo transfer, or of propagating the disease when attending a medical facility. Yet, the accuracy of these tests has been debated and it is still unclear how long any immunity lasts and if reinfection is possible [32]. Given the likelihood that this pandemic will continue for many months ahead, we must keep an open mind, and look for what is -and not for fear of what might be.…”

Section: Practical Considerations For Art Treatment Providersmentioning

confidence: 99%

COVID-19 and assisted reproductive technology services: repercussions for patients and proposal for individualized clinical management

Alviggi

Esteves

Orvieto

et al. 2020

Reprod Biol Endocrinol

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The prolonged lockdown of health services providing high-complexity fertility treatments -as currently recommended by many reproductive medicine entities-is detrimental for society as a whole, and infertility patients in particular. Globally, approximately 0.3% of all infants born every year are conceived using assisted reproductive technology (ART) treatments. By contrast, the total number of COVID-19 deaths reported so far represents approximately 1.0% of the total deaths expected to occur worldwide over the first three months of the current year. It seems, therefore, that the number of infants expected to be conceived and born -but who will not be so due to the lockdown of infertility services-might be as significant as the total number of deaths attributed to the COVID-19 pandemic. We herein propose remedies that include a prognostic-stratification of more vulnerable infertility cases in order to plan a progressive restart of worldwide fertility treatments. At a time when preventing complications and limiting burdens for national health systems represent relevant issues, our viewpoint might help competent authorities and health care providers to identify patients who should be prioritized for the continuation of fertility care in a safe environment.

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“…. https://doi.org/10.1101/2020 with FcγR signatures to a range of CoV. Amino acid (aa) alignment analyses of NP and S1 proteins 162 showed that there is 91% (NP) and 77% (S1) aa similarity between SARS-CoV-2 and SARS-CoV-1 163 proteins, while SARS-COV-2 and MERS share 47% (NP) aa similarity, hCoVs NL63 and 229E share 164 29% and 26% aa similarity, respectively in NP, and hCoV HKU and 229E share 28% and 27% in S1, 165 respectively (Extended Data Figure 3c Table 3) were screened for SARS-CoV-2 antigen-specific serological profiles (Extended Data 186 Figure 4).…”

Section: (Which Was Not Certified By Peer Review)mentioning

confidence: 99%

Distinct systems serology features in children, elderly and COVID patients

Selva

Sandt

Lemke

et al. 2020

Preprint

Self Cite

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54 55 SARS-CoV-2, the pandemic coronavirus that causes COVID-19, has infected millions worldwide, 56 causing unparalleled social and economic disruptions. COVID-19 results in higher pathogenicity and 57 mortality in the elderly compared to children. Examining baseline SARS-CoV-2 cross-reactive 58 coronavirus immunological responses, induced by circulating human coronaviruses, is critical to 59 understand such divergent clinical outcomes. The cross-reactivity of coronavirus antibody responses 60 of healthy children (n=89), adults (n=98), elderly (n=57), and COVID-19 patients (n=19) were 61 analysed by systems serology. While moderate levels of cross-reactive SARS-CoV-2 IgG, IgM, and 62 IgA were detected in healthy individuals, we identified serological signatures associated with SARS-63 CoV-2 antigen-specific Fcγ receptor binding, which accurately distinguished COVID-19 patients 64 from healthy individuals and suggested that SARS-CoV-2 induces qualitative changes to antibody Fc 65 upon infection, enhancing Fcγ receptor engagement. Vastly different serological signatures were 66 observed between healthy children and elderly, with markedly higher cross-reactive SARS-CoV-2 67 IgA and IgG observed in elderly, whereas children displayed elevated SARS-CoV-2 IgM, including 68receptor binding domain-specific IgM with higher avidity. These results suggest that less-experienced 69 humoral immunity associated with higher IgM, as observed in children, may have the potential to 70 induce more potent antibodies upon SARS-CoV-2 infection. These key insights will inform COVID-

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SARS‐CoV‐2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup

Cited by 729 publications

References 13 publications

Seroprevalence of antibodies against SARS-CoV-2 among health care workers in a large Spanish reference hospital

Seroprevalence of antibodies against SARS-CoV-2 among health care workers in a large Spanish reference hospital

COVID-19 and assisted reproductive technology services: repercussions for patients and proposal for individualized clinical management

Distinct systems serology features in children, elderly and COVID patients

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