2020
DOI: 10.1101/2020.12.12.422516
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SARS-CoV-2 RNA reverse-transcribed and integrated into the human genome

Abstract: SummaryProlonged SARS-CoV-2 RNA shedding and recurrence of PCR-positive tests have been widely reported in patients after recovery, yet these patients most commonly are non-infectious1–14. Here we investigated the possibility that SARS-CoV-2 RNAs can be reverse-transcribed and integrated into the human genome and that transcription of the integrated sequences might account for PCR-positive tests. In support of this hypothesis, we found chimeric transcripts consisting of viral fused to cellular sequences in pub… Show more

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Cited by 58 publications
(49 citation statements)
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References 49 publications
(41 reference statements)
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“…We next quantified the reads that partly mapped to the human genome and partly mapped to the SARS-CoV2 genome (see methods ). We found that nearly 0.05-1% of all viral reads are formed of hybrid sequences between host and virus RNAs, a frequency consistent with that recently reported by others 15,16 ( Fig. 1C ).…”
Section: Resultssupporting
confidence: 91%
See 2 more Smart Citations
“…We next quantified the reads that partly mapped to the human genome and partly mapped to the SARS-CoV2 genome (see methods ). We found that nearly 0.05-1% of all viral reads are formed of hybrid sequences between host and virus RNAs, a frequency consistent with that recently reported by others 15,16 ( Fig. 1C ).…”
Section: Resultssupporting
confidence: 91%
“…RNA-sequencing reads that partly align to the host genome and partly align to the viral genome are the signature of HVC events in RNA-sequencing datasets. Recent reports 15,16 suggesting the presence of HVC events in SARS-CoV2-infected cells have been interpreted as supporting viral integration into the human genome as a mechanism of viral persistence. To gain insights into the authenticity of these events, we re-analyzed the 3 available RNA-seq datasets from patients with COVID-19 (n=57 samples) and in vitro SARS-CoV2 infected cells (n=64 samples).…”
Section: Resultsmentioning
confidence: 99%
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“…Рекомбинация мРНК ретротранспозонов и РНК экзогенного неретровируса приводит к интеграции комплементарной к ней ДНК в геном [23], поэтому включение информации о mРНКвакцине в геном прививаемого не представляется невозможным. Применительно к SARS-Cov-2 в условиях in vitro уже подтверждено включение его генома в геном человека [30].…”
Section: векторные вакцины или мрнк-вакцины?unclassified
“…It is already clear, though mobile and exchangeable, that the human microbiome is part of our functional body (Ogunrinola et al 2020) and that a change in the microbiome, as well as viral gene transfer, is transformative to the body. At the time of writing there exists only a preprint of a yet-to-be-peer-reviewed paper about SARS-CoV-2 RNA fragments in the human genome (Zhang et al 2020) and little can be said about any consequences. However, it points towards the possibility of a deep future of the virus as part of the human genome.…”
Section: An Overviewmentioning
confidence: 99%