SARS-CoV-2 infections in infants in Haiti 2020-2021; evidence from a serological cohort

Louis, Rigan; Pu, Ruiyu; Logan, Tracy; Trimmer-Smith, Luke; Chamblain, Richard; Gallagher, Adriana; Rochars, Valery Madsen Beau De; Nelson, Eric J.; Cummings, Derek A. T.; Long, Maureen T.; Morris, J. Glenn

doi:10.1101/2022.03.17.22272561

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…A research ELISA targeting the Receptor Binding Domain (RBD) of the virus was used to detect exposure to SARS-CoV-2. The ELISA was adapted from a previously published protocol, which targets the RBD of the spike protein ( 34 , 35 ). Briefly, 96-well ELISA plates were coated with 1 μg/ml RBD protein diluted in carbonate/bicarbonate buffer (pH 9.6) and incubated at room temperature (RT) for 1 h. Each plate was blocked with 1X Tris-buffered saline (TBS) with 5% milk and incubated for 2 h. After blocking, each sample, diluted at a concentration of 1:100 in TBS-0.5% Tween, was added in duplicate to the plate.…”

Section: Methodsmentioning

confidence: 99%

The infected and the affected: A longitudinal study of the impact of the COVID-19 pandemic on schoolchildren in Florida

McKune

Acosta²,

Fujii³

et al. 2023

Front. Public Health

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ObjectivesTo identify risk factors associated with symptoms of anxiety, depression, and obsessive-compulsive disorder (OCD) among children during the 1st year of the COVID-19 pandemic.MethodsA longitudinal study with three cross-sectional timepoints [April 2020 (n = 273), October 2020 (n = 180), and April 2021 (n = 116)] was conducted at a K-12 public school in Florida. Infection and sero-positivity for SARS-CoV-2 was determined by molecular and serologic approaches. Adjusted odds ratios using mixed effect logistic regression models for symptom-derived indicators of anxiety, depression, and OCD in children in April 2021 are presented; past infection and seropositivity were included in the models.ResultsThe prevalence of anxiety, depression, or OCD moved from 47.1, to 57.2, to 42.2% across the three timepoints during the study. By endline of the study, in April 2021, non-white children were at higher risk for depression and OCD. Risk for anxiety, depression, and OCD was associated with students who lost a family member due to COVID-19 and who were identified as at-risk in previous timepoints. Rates of SARS-CoV-2 infection and seropositivity were low and not statistically associated with assessed outcomes.ConclusionsIn situations like the COVID-19 pandemic, targeted mental health interventions and screenings are needed in children and adolescents, especially among minority children.

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Section: Methodsmentioning

confidence: 99%

The infected and the affected: A longitudinal study of the impact of the COVID-19 pandemic on schoolchildren in Florida

McKune

Acosta²,

Fujii³

et al. 2023

Front. Public Health

Self Cite

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A Highly Sensitive Immunoassay for Determination of Immune Response to SARS-CoV-2 in Capillary Blood Samples

et al. 2022

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Throughout the pandemic, serological assays have been revealed as crucial for detecting previous exposures to the virus and determining the timing of antibody maintenance after vaccination or natural infection. This study aimed to develop an optimized enzyme-linked immunosorbent assay (ELISA)-based serology, which could be used in case of reagent shortages, such as that occurred in the beginning of this health emergency. As a result, we present a high-sensitive immunoassay for the determination of IgG levels in venous serum samples, using 2 μg/mL antigen (receptor-binding domain of the spike protein S1) for coating the plate and utilizing human samples at a dilution 1:1000. This method showed non-inferiority features versus a commercial kit, is less expensive, and has a higher spectrophotometric range that allows for a better quantification of the antibody titers. The optical density values before and after heating venous serum samples at 56 °C during 30 min was quite similar, showing that heat inactivation can be used to reduce the biohazardous risks while handling samples. Furthermore, we show that finger-stick capillary blood samples can also serve as a suitable source for IgG detection, bypassing the need for serum isolation and being suitable for point-of-care application (Pearson’s coefficient correlation with capillary serum was 0.95, being statistically significant).

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SARS-CoV-2 infections in infants in Haiti 2020-2021; evidence from a serological cohort

Cited by 2 publications

References 17 publications

The infected and the affected: A longitudinal study of the impact of the COVID-19 pandemic on schoolchildren in Florida

The infected and the affected: A longitudinal study of the impact of the COVID-19 pandemic on schoolchildren in Florida

A Highly Sensitive Immunoassay for Determination of Immune Response to SARS-CoV-2 in Capillary Blood Samples

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