SARS-CoV-2 Global Spreading Investigation Using Principal Component Analysis of Sequence Variants

Scherer, Christiane; Grover, James; Kammeraad, Darby; Rudy, Gabe; Scherer, Andreas

doi:10.23937/2378-3648/1410050

Cited by 2 publications

References 2 publications

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Data-driven approaches for genetic characterization of SARS-CoV-2 lineages

Mostefai

Gamache

Huang

et al. 2021

Preprint

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The genome of the Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2), the pathogen that causes coronavirus disease 2019 (COVID-19), has been sequenced at an unprecedented scale, leading to a tremendous amount of viral genome sequencing data. To understand the evolution of this virus in humans, and to assist in tracing infection pathways and designing preventive strategies, we present a set of computational tools that span phylogenomics, population genetics and machine learning approaches. To illustrate the utility of this toolbox, we detail an in depth analysis of the genetic diversity of SARS-CoV-2 in first year of the COVID-19 pandemic, using 329,854 high-quality consensus sequences published in the GISAID database during the pre-vaccination phase. We demonstrate that, compared to standard phylogenetic approaches, haplotype networks can be computed efficiently on much larger datasets, enabling real-time analyses. Furthermore, time series change of Tajima's D provides a powerful metric of population expansion. Unsupervised learning techniques further highlight key steps in variant detection and facilitate the study of the role of this genomic variation in the context of SARS-CoV-2 infection, with Multiscale PHATE methodology identifying fine-scale structure in the SARS-CoV-2 genetic data that underlies the emergence of key lineages. The computational framework presented here is useful for real-time genomic surveillance of SARS-CoV-2 and could be applied to any pathogen that threatens the health of worldwide populations of humans and other organisms.

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Data-driven approaches for genetic characterization of SARS-CoV-2 lineages

Mostefai

Gamache

Huang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Population Genomics Approaches for Genetic Characterization of SARS-CoV-2 Lineages

et al. 2022

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The genome of the Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2), the pathogen that causes coronavirus disease 2019 (COVID-19), has been sequenced at an unprecedented scale leading to a tremendous amount of viral genome sequencing data. To assist in tracing infection pathways and design preventive strategies, a deep understanding of the viral genetic diversity landscape is needed. We present here a set of genomic surveillance tools from population genetics which can be used to better understand the evolution of this virus in humans. To illustrate the utility of this toolbox, we detail an in depth analysis of the genetic diversity of SARS-CoV-2 in first year of the COVID-19 pandemic. We analyzed 329,854 high-quality consensus sequences published in the GISAID database during the pre-vaccination phase. We demonstrate that, compared to standard phylogenetic approaches, haplotype networks can be computed efficiently on much larger datasets. This approach enables real-time lineage identification, a clear description of the relationship between variants of concern, and efficient detection of recurrent mutations. Furthermore, time series change of Tajima's D by haplotype provides a powerful metric of lineage expansion. Finally, principal component analysis (PCA) highlights key steps in variant emergence and facilitates the visualization of genomic variation in the context of SARS-CoV-2 diversity. The computational framework presented here is simple to implement and insightful for real-time genomic surveillance of SARS-CoV-2 and could be applied to any pathogen that threatens the health of populations of humans and other organisms.

show abstract

SARS-CoV-2 Global Spreading Investigation Using Principal Component Analysis of Sequence Variants

Cited by 2 publications

References 2 publications

Data-driven approaches for genetic characterization of SARS-CoV-2 lineages

Data-driven approaches for genetic characterization of SARS-CoV-2 lineages

Population Genomics Approaches for Genetic Characterization of SARS-CoV-2 Lineages

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