SARS-CoV-2 envelope protein induces necroptosis and mediates inflammatory response in lung and colon cells through receptor interacting protein kinase 1

Baral, Budhadev; Saini, Vaishali; Tandon, Akrati; Singh, Siddharth; Rele, Samiksha; Dixit, Amit Kumar; Parmar, Hamendra Singh; Meena, Ajay Kumar; Jha, Hem Chandra

doi:10.1007/s10495-023-01883-9

Cited by 8 publications

(2 citation statements)

References 67 publications

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“…To understand the expression of specific markers at protein levels western blotting was performed as mentioned in our earlier work. 47 Briefly post completion of the treatment time (LPS + hydrogel treatment) cells were harvested and protein was isolated by RIPA buffer and an equal amount of protein was loaded into each well of a running gel. The separated proteins were then transferred to a 0.45 μM pore size nitrocellulose membrane and after blocking, these were incubated with a primary antibody followed by incubation with secondary antibody and visualization.…”

Section: Methodsmentioning

confidence: 99%

Design and synthesis of a nucleobase functionalized peptide hydrogel: in vitro assessment of anti-inflammatory and wound healing effects

Bhowmik,

Baral,

Rit

et al. 2024

Nanoscale

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Section: Methodsmentioning

confidence: 99%

Design and synthesis of a nucleobase functionalized peptide hydrogel: in vitro assessment of anti-inflammatory and wound healing effects

Bhowmik,

Baral,

Rit

et al. 2024

Nanoscale

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“…For transfection, 3 µg of plasmid was utilised. The transfection methodology was described in previous literature [15,17].…”

Section: Cell Culture Synchronisation Protocol Plasmid and Transfectionmentioning

confidence: 99%

Interplay of Aurora kinase A functional residues and Epstein-Barr Nuclear Antigen 1 in Epstein-Barr Virus associated Gastric cancer

Varshney,

Singh,

Kandpal

et al. 2024

Preprint

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Background Epstein-Barr virus (EBV), an oncogenic gamma-herpesvirus, belongs to group 1 carcinogen and is implicated in various cancers, including gastric cancer. Aurora Kinase A is a major mitotic protein kinase that regulates mitotic progression; overexpression and hyperactivation of AURKA commonly promote genomic instability in many tumours. However, the relationship of functional residues of AURKA and EBV in gastric cancer progression remains unknown. Results We reveal that AURKA overexpression and EBV infection induce aneuploidy in gastric epithelial cells. The AURKA (S89) N-terminal residue is critical for the centrosome maturation process in EBV-infected gastric epithelial cells. The kinase domain residues T287 and T288 of AURKA are essential for centrosome maturation and bipolar spindle formation in EBV-infected gastric cancer cells. We also show that AURKA 287/288 dm reduces the transcript expression of cell cycle markers involved in mitotic entry in EBV infection. This mutant also enhanced the protein expression of p53 and Rb, which was reduced in EBV infection and decreased the Survivin expression. Further, EBNA1, the latent gene of EBV, stabilises the AURKA in its wild-type form when it was mutated at the 89th position but unable to stabilise it when mutated at 287/288 position. These mutants also induce mitotic catastrophe by regulating the apoptosis and autophagy pathway in EBV infection. AURKA287/288 dm also promotes autophagosome formation even in EBV infection. Conclusion Thus, this study demonstrates that the AURKA kinase domain is essential for its functioning and progression of oncogenesis of EBV-infected gastric epithelial cells.

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The significance of recurrent de novo amino acid substitutions that emerged during chronic SARS-CoV-2 infection: an observational study

Ip,

Chu,

Chan

et al. 2024

eBioMedicine

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SARS-CoV-2 envelope protein induces necroptosis and mediates inflammatory response in lung and colon cells through receptor interacting protein kinase 1

Cited by 8 publications

References 67 publications

Design and synthesis of a nucleobase functionalized peptide hydrogel: in vitro assessment of anti-inflammatory and wound healing effects

Design and synthesis of a nucleobase functionalized peptide hydrogel: in vitro assessment of anti-inflammatory and wound healing effects

Interplay of Aurora kinase A functional residues and Epstein-Barr Nuclear Antigen 1 in Epstein-Barr Virus associated Gastric cancer

The significance of recurrent de novo amino acid substitutions that emerged during chronic SARS-CoV-2 infection: an observational study

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