SARS-CoV-2 3CLpro Main Protease Drives Cytoskeletal Reorganization and Tunnelling Nanotube Formation for Stealth Intercellular Infection
Christopher Overall,
Georgina Butler,
Marli Vlok
et al.
Abstract:To identify and characterize roles for SARS-CoV-2 3CLpro main protease in promoting viral infection and dissemination, we employed Inactive Catalytic Domain Capture and proteomics to identify host cell interactors and substrates in the interactome of 3CLpro. Of 259 3CLpro interactors, 145 were associated with the cytoskeleton and its organisation. We determined enzyme kinetic specificity constants for 139 3CLpro cut-sites in 43 interactors using a multiplex assay, identifying 29 efficiently-cleaved substrates … Show more
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