Sarracenia alata (Alph.Wood) Alph.Wood Microcuttings as a Source of Volatiles Potentially Responsible for Insects’ Respond

Abstract

Rare carnivorous plants representing the genus Sarracenia are perceived as very interesting to scientists involved in various fields of botany, ethnobotany, entomology, phytochemistry and others. Such high interest is caused mainly by the unique capacity of Sarracenia spp. to attract insects. Therefore, an attempt to develop a protocol for micropropagation of the Sarracenia alata (Alph.Wood) Alph.Wood, commonly named yellow trumpets, and to identify the specific chemical composition of volatile compounds of this plant in vitro and ex vivo was undertaken. Thus, the chemical volatile compounds excreted by the studied plant to attract insects were recognized with the application of the headspace solid-phase microextraction (HS-SPME) coupled with the GC-MS technique. As the major volatile compounds (Z)-3-hexen-1-ol (16.48% ± 0.31), (E)-3-hexen-1-ol acetate (19.99% ± 0.01) and β-caryophyllene (11.30% ± 0.27) were identified. Further, both the chemical assumed to be responsible for attracting insects, i.e., pyridine (3.10% ± 0.07), and whole plants were used in in vivo bioassays with two insect species, namely Drosophila hydei and Acyrthosiphon pisum. The obtained results bring a new perspective on the possibilities of cultivating rare carnivorous plants in vitro since they are regarded as a valuable source of bioactive volatile compounds, as including ones with repellent or attractant activity.

Morphological and olfactory tree traits influence the susceptibility and suitability of the apple species Malus domestica and M. sylvestris to the florivorous weevil Anthonomus pomorum (Coleoptera: Curculionidae)

“…Acetophenone can repel Drosophila suzukii from raspberries ( Renkema & Smith, 2020 ) and can even have insecticidal effects ( Dettner et al, 1992 ). Pyridine, the second most-emitted plant volatile from the blossom buds has rarely been detected from plant odors, but it was reported for grape leaves ( Giacomuzzi et al, 2017 ), bearberry plants ( Radulović, Blagojević & Palić, 2010 ), and floral volatiles of Eriotheca longitubulosa ( MacFarlane, Mori & Purzycki, 2003 ) and is known to attract flies and thrips ( Łyczko et al, 2021 ).…”

Secondary Metabolites with Biomedical Applications from Plants of the Sarraceniaceae Family

“…p-cymene is a volatile compound extracted from S. alata [ 45 ]. It shows anti-inflammatory activity, being able to modulate cytokine (TNF-α, IL-1β, IL-6) production in vitro and in vivo [ 200 ] and exhibits cytotoxic activity against a large variety of cancer cell lines such as breast, ovarian, melanoma, colorectal and hepatocellular [ 201 ].…”

“…Among the sesquiterpenes found in S. alata ( Table 1 ), the predominant ones are β-caryophyllene and α-bergamotene ( Figure 4 ) [ 45 ]. β-caryophyllene is a volatile compound of essential oils from many herbs and spices [ 245 ] including Cannabis sativa [ 246 ].…”

“…Pyridine is a volatile compound from S. alata [ 45 ]. It is a scaffold that has been consistently incorporated in a diverse range of drug candidates to sharpen their potency and to improve pharmacokinetics [ 271 ].…”

Reactive Oxygen Species Initiate Defence Responses of Potato Photosystem II to Sap-Sucking Insect Feeding

“…Thus, when plants emit a particular compound, it is clearly detectable to particular insects that utilize the plant [ 9 , 10 ]. The aim of the released VOC by plants in response to an insect attack includes directly preventing herbivores, indirectly attracting natural enemies of attackers, and priming defences of intact organs on the same plant [ 11 , 12 , 13 ]. Miscellaneous molecular mechanisms regulate the interactions between plants and herbivore insects and the concomitant compensatory processes in the plants [ 4 , 14 , 15 ].…”