Saringosterol Acetate Isolated from <i>Sargassum fusiformis</i> Induces Mitochondrial‐Mediated Apoptosis in MCF‐7 Breast Cancer Cells

Li, Xue; Zhou, Dexi; Li, Fangtong; Jiang, Yunfei; Dai, Yulin; Jeon, You‐Jin

doi:10.1002/cbdv.202100848

Cited by 3 publications

(8 citation statements)

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“…Sterols are an important class of active compounds. [17] Based on the results, SA markedly decreased lipid accumulation and triglyceride contents. In a dosedependent manner, SA also regulated adipogenesisrelated proteins in the adipocytes.…”

Section: Discussionmentioning

confidence: 92%

“…S. fusiforme is traditional edible brown seaweed that has been used for thousands of years in Korea and China because of its various activities. Sterols are an important class of active compounds [17] . Based on the results, SA markedly decreased lipid accumulation and triglyceride contents.…”

Section: Discussionmentioning

confidence: 98%

“…[38] SA was isolated as described in our previous study. [17] Briefly, S. fusiforme was extracted in 70 % ethanol for 24 h at 40 °C. The resulting solution was evaporated, and separated into water and hexane layers.…”

Section: Methodsmentioning

confidence: 99%

“…Wild type S. fusiforme was obtained from east Jeju Island, Korea in May 2019, which of the harvested position is 33.440220“ N/126.942954” E. Repositories were deposited in Jeju National University (Jeju, Korea) [38] . SA was isolated as described in our previous study [17] . Briefly, S. fusiforme was extracted in 70 % ethanol for 24 h at 40 °C.…”

Section: Methodsmentioning

confidence: 99%

“…[16] In our previous study, SA isolated from hexane extract of S. fusiforme was shown to exhibit anticancer properties on MCF-7 breast cancer cells. [17] To the best of our knowledge, no previous reports have described the anti-obesity effects of SA on adipocytes.…”

Section: Introductionmentioning

confidence: 99%

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Treatment of Obese Zebrafish with Saringosterol Acetate through AMP Activated Protein Kinase Pathway

Zhou

et al. 2022

Chemistry & Biodiversity

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Object: Edible Brown Seaweed Sargassum fusiforme (Harvey) Setchell, 1931 abbreviated as Sargassum fusiforme was used for folk medical therapy in East Asia countries over five hundred years. Saringosterol acetate (SA) was isolated from S. fusiforme in our previous study and indicated various effects. However, anti-obesity activity of SA and its mechanism still unknown. Method: The inhibitory effect of SA, isolated from S. fusiforme, on adipogenesis in 3T3-L1 adipocytes was investigated in vitro and in zebrafish model. Cell toxicity, differentiation, signaling pathway, and lipid accumulation of SA treated 3T3-L1 adipocytes were determined. The body weight and triglyceride content of diet-induced obese (DIO) adult male zebrafish were measured from 12 to 17 weeks after fertilization. Result: SA attenuated the differentiation of cells and reduced lipid accumulation, and triglyceride content in the 3T3-L1 adipocytes. During the differentiation of adipocytes, SA suppressed fat accumulation and decreased the expression of signal factors responsible for adipogenesis. In SA-treated adipocytes, while fatty acid synthetase was downregulated, AMP-activated protein kinase (AMPK) was upregulated. Furthermore, SA suppressed body weight and triglyceride content in DIO zebrafish. Conclusion: SA is a potential therapeutic agent in the management of metabolic disorders, such as obesity.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Treatment of Obese Zebrafish with Saringosterol Acetate through AMP Activated Protein Kinase Pathway

Zhou

et al. 2022

Chemistry & Biodiversity

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Molecular mechanisms of Rhizoma Dioscoreae in breast cancer treatment: a network pharmacology and experimental verification

Wang,

Gao,

Zhang

et al. 2024

Preprint

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Background Breast cancer poses a significant threat on human health. Rhizoma Dioscoreae (RD), a traditional Chinese medicinal herb, has shown positive effect in breast cancer treatment; however, the underlying molecular mechanisms remain unknown. Methods We identified the active pharmaceutical ingredients (APIs) in RD and their potential targets using the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. Targets related to breast cancer were gathered from GeneCards and Online Mendelian Inheritance in Man (OMIM) database. We constructed RD API-gene interaction network using Cytoscape software. Protein-protein interaction (PPI) data were obtained from the Search Tool for the Retrieval of Interacting Genes (STRING) database. Key APIs and hub genes were screened via network topological parameters. Gene Ontology (GO) and Kyoto Encyclopedia of Genes (KEGG) pathway enrichment analyses were conducted. Molecular docking and in vitro experiments were performed to validate our network pharmacology findings. Results We identified 12 APIs in RD and 28 genes overlapped between RD and breast cancer. Molecular docking showed stable binding between key ingredients and hub genes. Stigmasterol and diosgenin served as the most critical APIs. Further experimental findings demonstrated that RD inhibited breast cancer via downregulating HIF-1α, RELA, VEGFA, PGR, and NCOA1, critical in tumor angiogenesis and hormone-mediated signaling. Conclusion RD might inhibit breast cancer angiogenesis and hormone-mediated oncogenesis by downregulating HIF-1α, RELA, VEGFA, PGR, and NCOA1, which provides more evidence for the application of RD in the management of breast cancer.

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The Effect of Sargassum Angustifolium-derived AgNPs on Apoptosis-associated Genes in Acute Lymphoblastic Leukemia Cells

Obeidi,

Babolei,

Khamisipour

et al. 2023

Asian Pac J Cancer Prev

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Introduction: Acute lymphoblastic leukemia (ALL) is among the most prevalent child cancers. Moreover, chemotherapy and bone marrow transplantation have failed to secure the survival of patients in some cases. Various researches have revealed that glycogen synthase kinase 3 (GSK-3) inhibitors can contain the growth of some cancers. Furthermore, inositol trisphosphate receptor (IP3R) exists in all cell types and is implicated in metastasis. The application of organic, natural substances offers new prospects for the treatment of this condition. Accordingly, the aim of this study was to examine the silver nanoparticles synthesized from Sargassum Angustifolium on the expression of IP3 and GSK receptors in ALL Jurkat cells. Methods: We isolated Sargassum Angustifolium extract and mixed it with silver nanoparticles (NPs) and treated the cells with the mixture. The changes in the expression of GSKα , IP3R3 and GSKβ genes in the Jurkat cell line were studied. In this research, quantitative mRNA expression of the target gene was measured using a real-time polymerase chain reaction (PCR). Hypoxanthine phosphoribosyltransferase (HPRT) genes were studied as the internal control. The experiments were replicated 3 times. Data analysis was performed through one-way ANOVA and t-test. The significance level was considered less than 0.05. Results: The results of this study revealed that different concentrations of the extracts significantly decreased the expression levels of GSKα , IP3R3 and GSKβ gene in Jurkat cells compared to control groups. The combination of algae extract and AgNPs was consistently the most effective group. Conclusion: silver nanoparticles synthesized from sargassum algae in the Persian Gulf could be utilized to treat ALL cancers and even other tumors.

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Saringosterol Acetate Isolated from Sargassum fusiformis Induces Mitochondrial‐Mediated Apoptosis in MCF‐7 Breast Cancer Cells

Cited by 3 publications

References 32 publications

Treatment of Obese Zebrafish with Saringosterol Acetate through AMP Activated Protein Kinase Pathway

Treatment of Obese Zebrafish with Saringosterol Acetate through AMP Activated Protein Kinase Pathway

Molecular mechanisms of Rhizoma Dioscoreae in breast cancer treatment: a network pharmacology and experimental verification

The Effect of Sargassum Angustifolium-derived AgNPs on Apoptosis-associated Genes in Acute Lymphoblastic Leukemia Cells

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