Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-κB) and induces cytokine production via Toll-like receptors

Chen, Xiaoming; Yu, Guoqing; Fan, Sairong; Bian, Manman; Ma, Huijun; Lü, Jianxin; Jin, Liqin

doi:10.1016/j.carbpol.2014.01.056

Cited by 43 publications

(19 citation statements)

References 26 publications

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“…Phosphorylation of IκBα at Ser 32 and Ser 36 , and the phosphorylation of IκBβ at Ser 19 and Ser 23 , which are targeted by the IKKβ subunit of the IKK complex in the course of the canonical NF-κB signaling [31], leading to the release releases of active NF-κB, which then translocates to the nucleus, binds to specific DNA enhancer sequences (κB binding sites), and activates pro-inflammatory target gene transcription [32]. Chen et al reported that S. fusiforme polysaccharides can stimulate the secretion of cytokines via the TLR2/4 and CD14 receptors and subsequent activation of P65, measured as an increase in nuclear P65 following polysaccharide treatment [9]. However, less information concerning the detail analysis of the relationship between the polysaccharides and the key proteins involved in the NF-κB/MAPK signaling pathway in the presence of S. fusiforme polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

“…The cells were collected, washed twice with ice-cold PBS, and then lysed in RIPA lysis buffer [9]. Equal amounts of total protein from the different cell extracts were separated by SDS-PAGE using 8–10% gels.…”

Section: Methodsmentioning

confidence: 99%

“…Accumulated evidence shows that polysaccharides extracted from S. fusiforme exhibit numerous biological activities, including anti-tumor, anti-oxidation, anticoagulant, immuno-stimulating and anti-aging activities [3,4,5]. Among these, the immuno-stimulating activity of the polysaccharides has been widely studied, and it has been shown to increase the weight of the thymus and spleen [6], alter gene expression in peritoneal macrophages [7], enhance the growth of lymphocytes, and promote the productions of IgG and inflammatory cytokines [8,9]. S. fusiforme polysaccharide exerts its immuno-stimulating activity through the TLRs (toll-like receptors)/NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway [9].…”

Section: Introductionmentioning

confidence: 99%

“…Among these, the immuno-stimulating activity of the polysaccharides has been widely studied, and it has been shown to increase the weight of the thymus and spleen [6], alter gene expression in peritoneal macrophages [7], enhance the growth of lymphocytes, and promote the productions of IgG and inflammatory cytokines [8,9]. S. fusiforme polysaccharide exerts its immuno-stimulating activity through the TLRs (toll-like receptors)/NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway [9]. In this study, a novel polysaccharide fraction was isolated from S. fusiforme .…”

Section: Introductionmentioning

confidence: 99%

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Sargassum Fusiforme Polysaccharide SFP-F2 Activates the NF-κB Signaling Pathway via CD14/IKK and P38 Axes in RAW264.7 Cells

Chen

Liu

et al. 2018

Marine Drugs

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Sargassum fusifrome is considered a “longevity vegetable” in Asia. Sargassum fusifrome polysaccharides exhibit numerous biological activities, specially, the modulation of immune response via the NF-κB signaling pathway. However, the precise mechanisms by which these polysaccharides modulate the immune response through the NF-κB signaling pathway have not been elucidated. In this study, we purified and characterized a novel fraction of Sargassum fusifrome polysaccharide and named it SFP-F2. SFP-F2 significantly upregulated the production of the cytokines TNF-α, IL-1β and IL-6 in RAW264.7 cells. It also activated the NF-κB signaling pathway. Data obtained from experiments carried out with specific inhibitors (PDTC, BAY 11-7082, IKK16 and SB203580) suggested that SFP-F2 activated the NF-κB signaling pathway via CD14/IKK and P38 axes. SFP-F2 could therefore potentially exert an immune-enhancement effect through inducing the CD14/IKK/NF-κB and P38/NF-κB signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sargassum Fusiforme Polysaccharide SFP-F2 Activates the NF-κB Signaling Pathway via CD14/IKK and P38 Axes in RAW264.7 Cells

Chen

Liu

et al. 2018

Marine Drugs

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“…[7] NF-κB is a common cytokine which can be activated by many bacteria and viruses. [8] NF-κB has been shown to induce the excessive release of inflammatory cytokines including TNF-α, IL-1β, and IL-6, and then promote significant accumulation of neutrophils in inflammatory regions, thereby causing inflammatory responses. NF-κB-induced activation of TNF-α, IL-1β, and IL-6 can also up-regulate NF-κB.…”

Section: Discussionmentioning

confidence: 99%

Effects of aspirin on the expression of nuclear factor-κB in a rat model of acute pulmonary embolism

Wang¹,

Jiang²,

Zhang³

et al. 2014

World Journal of Emergency Medicine

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BACKGROUND: Acute pulmonary embolism (APE) is a disorder involving the pulmonary circulation resulting from a blockage of the pulmonary artery. The present study aimed to investigate the effects of aspirin on the nuclear factor-κB (NF-κB) activity in a rat model of APE. METHODS:A total of 108 healthy male Sprague-Dawley rats were randomly assigned into six groups (n=18 rats per group): control group, sham operation group, APE model group, and low-, medium-and high-dose aspirin groups. Six, 24, and 72 hours after the induction of APE, rats in the low-, medium-and high-dose aspirin groups were given aspirin at a respective daily dose of 150, 300, and 600 mg/kg by gavage for three consecutive days. Rats in the other groups were treated with equal volumes of normal saline. Six rats in each group were anesthetized with 10% chloral hydrate solution at each time point, and then the lung tissues were collected and analyzed using immunohistochemical staining. RESULTS:Positive immunohistochemical staining was present in the bronchial epithelial cells, alveolar cells, macrophages, and surrounding bronchial smooth muscle cells. When compared with the APE model group, the number of positive cells was signifi cantly lower in the other groups at each time point (P<0.001). Statistically signifi cant differences were also observed among the aspirin-treated groups at 6 hours (P<0.05, P<0.001). Compared with the APE model group, NF-κB protein expression was reduced in the other groups at each time point (P<0.05, P<0.001). Rats from the APE model group had thrombosis, damaged alveolar walls, and pulmonary hemorrhage, along with different degrees of infl ammatory cellular infi ltration at each time point. However, pathological changes such as pulmonary hemorrhage and infi ltration of infl ammatory cells were attenuated after the aspirin treatment.CONCLUSION: Aspirin can signifi cantly inhibit NF-κB activity in the lung of rats with APE in a dose-dependent manner, and can alleviate lung injury after APE.

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Polysaccharides and immune function

Duttaroy

2021

Evidence-Based Nutrition and Clinical Evidence of Bioactive Foods in Human Health and Disease

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Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-κB) and induces cytokine production via Toll-like receptors

Cited by 43 publications

References 26 publications

Sargassum Fusiforme Polysaccharide SFP-F2 Activates the NF-κB Signaling Pathway via CD14/IKK and P38 Axes in RAW264.7 Cells

Sargassum Fusiforme Polysaccharide SFP-F2 Activates the NF-κB Signaling Pathway via CD14/IKK and P38 Axes in RAW264.7 Cells

Effects of aspirin on the expression of nuclear factor-κB in a rat model of acute pulmonary embolism

Polysaccharides and immune function

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