Sarcocystis spp. Infection in two Red Panda Cubs (Ailurus fulgens)

Zoll, Whitney M.; Needle, David B.; French, Stephanie; Lim, Ailam; Bolin, Steven R.; Langohr, Ingeborg M.; Agnew, Dalen

doi:10.1016/j.jcpa.2015.04.009

Cited by 7 publications

(5 citation statements)

References 15 publications

(17 reference statements)

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“…No inflammation was found around the sarcocyst-like cysts, indicating that the infection was subclinical. To date, only one study of sarcocystosis outbreak in red panda cubs has been document (Zoll et al, 2015), it maybe attribute to S. neurona or S. dasypi .…”

Section: Discussionmentioning

confidence: 99%

“…The red panda ( Ailurus fulgens ) is an endangered species that primarily lives in temperate forests of China, Bhutan, India, Myanmar, and Nepal (Hunter, 1991). To date, only one study reported the presence of antibodies to T. gondii in red pandas (Qin et al, 2007), whereas other reports found no evidence of T. gondii infection in red panda by serology, molecular or histology methods (Langan et al, 2000; Zoll et al, 2015; Loeffler et al, 2007). There is no direct evidence to confirm that they can serve as an intermediate host of T. gondii .…”

Section: Introductionmentioning

confidence: 99%

“…These species undergo multiple development stages within different host cells and can found incidentally in the tissues of mammals, birds and reptiles (Dubey et al, 2016). However, there is only one report documented schizonts of a Sarcocystis -like organism infection in red panda (Zoll et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Evidence of red panda as an intermediate host of Toxoplasma gondii and Sarcocystis species

Yang

Dong

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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Toxoplasma gondii has been found to infect almost all warm-blooded animals; however, some hosts lack direct evidence of T. gondii infection. The red panda ( Ailurus fulgens ) is an endangered species that mainly lives in temperate forests of South Asia. Here, T. gondii infection in red pandas from zoos in China were reported. Antibodies to T. gondii were found in 14.3% (2/14) of red pandas via the modified agglutination test (MAT) with a cut-off titer of 1:25. One viable T. gondii strain was isolated from tissues of red panda and designated as TgRedpandaCHn1. DNA from tachyzoites obtained from cell culture was characterized by PCR-RFLP with 10 markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29–2, L358, PK1, and Apico) and virulence genes of ROP5 and ROP18. The results indicate that this isolate belonged to ToxoDB genotype #20. The ROP18/ROP5 genotype combination predicated that this strain is non-lethal to mice, which is supported by the infection in mice. T. gondii tissue cysts were readily formed and mice survived. Tissue cysts observed in the histopathological sections of the tongue and diaphragm of one red panda were speculated as sarcocysts, but not T. gondii base on morphological characteristics. To our knowledge, this study is the first to report on the isolation of T. gondii from red panda. Additionally, this report provides direct evidence of red panda as an intermediate host of T. gondii and Sarcocystis species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evidence of red panda as an intermediate host of Toxoplasma gondii and Sarcocystis species

Yang

Dong

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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“…Thin sections (70-nm thickness) were cut with a PTXL ultramicrotome (RMC, Boeckeler Instruments, Tucson, AZ, USA), placed on 200 mesh copper grids, stained with uranyl acetate and lead citrate, and imaged using a JEOL 100CX Transmission Electron Microscope (JEOL, Tokyo, Japan) at a 100 kV accelerating voltage, as previously described. 41…”

Section: Methodsmentioning

confidence: 99%

Necrotizing Ventriculitis in Fledgling Chimney Swifts (Chaetura Pelagica) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1)

et al. 2019

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Five chimney swift fledglings died following a progressive loss of appetite and condition while being cared for by an experienced wildlife rehabilitator. All animals had severe necrotizing and heterophilic ventriculitis, with myriad epithelial cells characterized by karyomegaly with intranuclear inclusion bodies. Transmission electron microscopy showed distention of epithelial cell nuclei and chromatin peripheralization by nonenveloped, icosahedral, 75- to 85-nm-diameter virions. Degenerate nested PCR for a highly conserved region of the adenovirus DNA polymerase gene was positive. BLAST analysis of the amplicon sequence indicated the presence of a novel adenovirus, with 74% homology to Antarctic penguin adenoviruses and 72% homology to a bat adenovirus, at low query coverages of only 65% and 63%, respectively. BLAST analysis of the predicted amino acid sequence generated the highest scores for squamate adenoviruses at 100% query coverage. Based on phylogenetic analysis of the partial amino acid sequence of the DNA polymerase, the chimney swift virus was a novel adenovirus most closely related to the Atadenovirus genus. Using a probe based on the novel viral sequence, DNA in situ hybridization identified viral nucleic acid in the nucleus. While the tentatively named chimney swift adenovirus-1 (CsAdV-1) is so far classified with the Atadenoviruses, it is relatively divergent from other members of that genus and may represent the first identified member of a new genus of Adenoviruses.

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“…O T. gondii possue um ciclo biológico com dois tipos de hospedeiros: a) hospedeiro definitivo, os felídeos, com destaque para o gato doméstico; e b) intermediário: todos animais endotérmicos, a exemplo de roedores, cães, suínos, ovinos, caprinos e aves, podendo parasitar até mesmo mamíferos marinhos (Zoll et al, 2015, Dubey et al, 2016. No intestino delgado do hospedeiro definitivo, ocorre a produção de oocistos através da reprodução sexuada de gametas do parasito (MILLER et al, 1972).…”

Section: Toxoplasmas Gondii Em Caprinosunclassified

Aspectos epidemiológicos de enfermidades causadas por vírus e protozoários de animais de produção no Brasil e Moçambique

Mataca¹

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Os vírus da artrite-encefalite caprina (CAEV) e língua azul (BTV) e os parasitas Neospora caninum e Toxoplasma gondii podem ser importantes agentes infecciosos para caprinos leiteiros. Além disso, o herpesvírus bovino 1 (BHV-1) e o vírus da estomatite vesicular (VSV) também podem ocasionalmente infectar caprinos e causar problemas na produção. Ainda, a Peste Suína Africana (PSA) é uma doença infecciosa transfronteiriça que afeta de forma insidiosa os suínos domésticos e causa grandes prejuízos para a indústria suinícola. Este estudo objetivou avaliar a prevalência, os fatores de risco para infecção viral (CAEV, BTV, BHV-1 e VSV) e os protozoários (T. gondii e N. caninum) (capítulo 1); bem como a disposição natural dos grupos soropositivos quanto à sua similaridade multivariada em fazendas de caprinos leiteiros comerciais na Zona da Mata de Minas Gerais, Brasil e a distribuição espacial, temporal e a tendência temporal da PSA em Moçambique (capítulo 2). A prevalência média de 413 amostras de soro encontrada no capítulo 1 foi de 65% (269/413) e 47,1% (195/413) para os parasitas N. caninum e T. gondii, e 35% (145/413) e 24% (99/413) para BTV e CAEV, respectivamente. Todas as amostras foram soronegativas para BHV- 1 e VSV. Fatores de risco significativos foram encontrados apenas na infecção por T. gondii e CAEV. As fazendas com mais casos positivos de CAEV, N. caninum e T. gondii estão conectadas por fronteiras, demonstrando possível interação espacial da soropositividade dos rebanhos. No capítulo II foi demostrado que Moçambique, entre os 21 anos de estudos, notificou 28.624 casos de PSA distribuídos para as três regiões do país, com destaque para a região norte com 64,9% do total de casos. A maior incidência relativa (40,8%), foi demostrada na província de Cabo Delgado, e a tendência temporal demostrou que a maioria das províncias têm tendência decrescente de casos. Espera-se alertar aos programas oficiais de vigilância, a importância de controlar essas infecções virais e protozoárias na região de produção de leite caprino no Brasil, mas também, para a PSA em Moçambique, com atuação permanente entre as fronteiras das províncias e países, de forma a evitar a propagação viral para regiões vulneráveis. Palavras-chave: Risco epidemiológico. Vigilância geográfica. Defesa sanitária animal. Cluster. Vírus. Protozoários.

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Sarcocystis spp. Infection in two Red Panda Cubs (Ailurus fulgens)

Cited by 7 publications

References 15 publications

Evidence of red panda as an intermediate host of Toxoplasma gondii and Sarcocystis species

Evidence of red panda as an intermediate host of Toxoplasma gondii and Sarcocystis species

Necrotizing Ventriculitis in Fledgling Chimney Swifts (Chaetura Pelagica) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1)

Aspectos epidemiológicos de enfermidades causadas por vírus e protozoários de animais de produção no Brasil e Moçambique

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