Sarcocystis spp. diversity in the roe deer (Capreolus capreolus) from Lithuania and Spain

Rudaitytė-Lukošienė, Eglė; Cuevas, Guillermo E. Delgado de las; Prakas, Petras; Calero‐Bernal, Rafael; Martínez-González, Manuel; Strazdaitė-Žielienė, Živilė; Servienė, Elena; Habela, M.; Butkauskas, Dalius

doi:10.1007/s00436-020-06603-9

Cited by 14 publications

(20 citation statements)

References 24 publications

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“…aretiecanis 95.8–96.3 KU820984 ; MK420017 ( Hu et al, 2016 ; Gjerde et al, 2020 ) Sarcocystis sp. MT737952 Fox 16 Fox16 PCR 639 S. miescheriana 100 MH404232 Gazzonis et al (2019) S. miescheriana MT737934 Fox16 clone1-Fox16 clone8 664 S. miescheriana 99.9–100 MH404232 Gazzonis et al (2019) S. miescheriana MW020901- MW020903 Fox 46 Fox46 PCR 617 S. hjorti 100 GQ250990 Dahlgren and Gjerde (2010a) S. hjorti MT737935 Fox 47 Fox47 PCR 638 S. gracilis 100 MN334289 Rudaitytė-Lukošienė et al (2020) S. gracilis MT737936 Fox47 clone1-Fox47 clone4 675 S. gracilis 99.7–100 MN334289 Rudaitytė-Lukošienė et al (2020) S. gracilis MW020904- MW020907 Fox47 clone5 647 S. tenella …”

Section: Resultsmentioning

confidence: 99%

“…tenella 98.6–98.9 KU820983 , KP263759 ( Kolenda et al, 2015 ; Hu et al, 2016 ) Sarcocystis sp. MW020916 Fox 115 Fox115 PCR 637 S. gracilis 100 MN334289 Rudaitytė-Lukošienė et al (2020) S. gracilis MT737939 Fox 120 Fox120 PCR 621 S. tenella 100 KP263759 Kolenda et al (2015) S. tenella MT737940 Fox120 clone1, 647 S. tenella 99.2–99.7 MF039329 , KP263756 , ( Kolenda et al, 2015 ; Hu et al, 2017 ) S. tenella MW020919, MW020920, MW020922 Fox120 clone7, KP263759 Fox120 clone9 Fox120 clone8, Fox120 clone10 645–647 S. tenella/S. capracanis 98.6–98.9 KP263756 , KP263759 ( Kolenda et al, 2015 ; Hu et al, 2016 ) Sarcocystis sp.…”

Section: Resultsmentioning

confidence: 99%

“… MW020925, MW020926 Dog 5 Dog5 clone1, 663 S. capreolicanis 99.4–99.5 KY019029 , MN334253 ( Gjerde et al, 2017a ; Rudaitytė-Lukošienė et al, 2020 ) S. capreolicanis MT737953, MT737956 Dog5 clone5 Dog5 clone2, Dog5 clone3 675 S. gracilis 99.1–99.4 MN334289 Rudaitytė-Lukošienė et al (2020) S. gracilis MT737954, MT737955 Dog 6 Dog6 clone1, Dog6 clone3 656 S. linearis/S. taeniata 98.7–99 MN334294, KU753890 ( Prakas et al, 2016 ; Rudaitytė-Lukošienė et al, 2020 ) Sarcocystis sp. MT737957, MT737958 Dog6 clone9 675 S. gracilis 99.8 MN334289 Rudaitytė-Lukošienė et al (2020) S. gracilis MT737959 …”

Section: Resultsmentioning

confidence: 99%

“… MT737851- MT737854 Deer 13 D13 clone1-D13 clone3, 658–660 S. linearis/S. taeniata 97.9–99.9 KY973371 , KY973372 , KU753890 , KT626602 , MN334301, KF831293 ( Gjerde, 2014a ; Prakas et al, 2016 ; Reissig et al, 2016 ; Gjerde et al, 2017b ; Rudaitytė-Lukošienė et al, 2020 ) Sarcocystis sp. MT737855- MT737857, MT737859 D13 clone 5 D13 clone4 654 S. hjorti 99.2 KF831294 Gjerde (2014a) S. hjorti MT737858 Deer 14 D14 PCR 529 S. pilosa 100 LC466183 Irie et al (2019) S. pilosa MT737860 D14 clone1 653 S. pilosa 99.7 LC466183 Irie et al (2019) S. pilosa MT737861 D14 clone2, 654 S. hjorti 98.9–99.9 KF831294 Gjerde (2014a) ...…”

Section: Methodsmentioning

confidence: 99%

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Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Basso

Rojas

Buob

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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Red deer ( Cervus elaphus ) carcasses showing grey-greenish discolouration have been increasingly observed in the canton of Grisons, Switzerland. We investigated whether Sarcocystis infections were associated with this pathology, and whether wild and domestic canids were involved in their transmission. Meat from affected red deer ( n = 26), faeces and intestines from red foxes ( Vulpes vulpes ) ( n = 126), and faeces from hunting dogs ( n = 12) from the region, were analysed. Eosinophilic myositis and/or fasciitis were diagnosed in 69% of the deer, and sarcocysts were observed in 89% of the animals. Molecular typing targeting a ~700bp variable region of the 18S rRNA gene revealed Sarcocystis hjorti in 73%, S. venatoria/S. iberica in 54%, S. linearis/S. taeniata in 12%, S. pilosa in 8% and S. ovalis in 4% of the deer samples. No inflammatory changes were observed in red deer carcasses with normal appearance ( n = 8); however, sarcocysts were observed in one sample, and S. hjorti , S. venatoria/S. iberica or S. silva DNA was detected in five samples. Sarcocystis oocysts/sporocysts were observed in 11/106 faecal and 6/20 intestinal fox samples, and in 2/12 canine samples. Sarcocystis tenella ( n = 8), S. hjorti ( n = 2), S. gracilis ( n = 2), and S. miescheriana ( n = 1) were identified in foxes, and S. gracilis ( n = 2), S. capreolicanis ( n = 1) and S. linearis/S. taeniata ( n = 1) in dogs. This study provides first molecular evidence of S. pilosa and S. silva infection in red deer and S. linearis/S. taeniata in dogs and represents the first record of S. ovalis transmitted by corvids in Central Europe. Although Sarcocystis species infecting red deer are not regarded as zoonotic, the affected carcasses can be declared as unfit for human consumption due to the extensive pathological changes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Basso

Rojas

Buob

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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“…Furthermore, a recent study of six Sarcocystis spp. infecting roe deer showed that COI intraspecific variability coincided with intraspecific variability of the 18S rRNA gene, rendering species-, not gene-specific genetic variability ( Rudaitytė-Lukošienė et al, 2020 ). Future sampling however has to show whether low variability is true for the entire geographical distribution and host range of S .…”

Section: Discussionmentioning

confidence: 99%

Description of Sarcocystis scandentiborneensis sp. nov. from treeshrews (Tupaia minor, T. tana) in northern Borneo with annotations on the utility of COI and 18S rDNA sequences for species delineation

Pérez

Wibbelt

Brinkmann

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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Sarcocystis scandentiborneensis sp. nov. was discovered in histological sections of striated musculature of treeshrews ( Tupaia minor , T . tana ) from Northern Borneo. Sarcocysts were cigar-shaped, 102 μm–545 μm long, and on average 53 μm in diameter. The striated cyst wall varied in thickness (2–10 μm), depending on whether the finger-like, villous protrusions (VP) were bent. Ultrastructurally, sarcocysts were similar to wall type 12 but basal microtubules extended into VPs that tapered off with a unique U-shaped, electron-dense apical structure. In phylogenetic trees of the nuclear 18S rRNA gene, S . scandentiborneensis formed a distinct branch within a monophyletic subclade of Sarcocystis spp. with (colubrid) snake-rodent life cycle. We mapped all intraspecific (two haplotypes) and interspecific nucleotide substitutions to the secondary structure of the 18S rRNA gene: in both cases, the highest variability occurred within helices V2 and V4 but intraspecific variability mostly related to transitions, while transition/transversion ratios between S . scandentiborneensis , S . zuoi , and S . clethrionomyelaphis were skewed towards transversions. Lack of relevant sequences restricted phylogenetic analysis of the mitochondrial Cytochrome C oxidase subunit I (COI) gene to include only one species of Sarcocystis recovered from a snake host ( S. pantherophisi ) with which the new species formed a sister relationship. We confirm the presence of the functionally important elements of the COI barcode amino acid sequence of S . scandentiborneensis , whereby the frequency of functionally important amino acids (Alanine, Serine) was markedly different to other taxa of the Sarcocystidae. We regard S . scandentiborneensis a new species, highlighting that structurally or functionally important aspects of the 18S rRNA and COI could expand their utility for delineation of species. We also address the question why treeshrews, believed to be close to primates, carry a parasite that is genetically close to a Sarcocystis lineage preferably developing in the Rodentia as intermediate hosts.

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First molecular characterization of Sarcocystis miescheriana in wild boars (Sus scrofa) from Latvia

et al. 2020

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Sarcocystis spp. diversity in the roe deer (Capreolus capreolus) from Lithuania and Spain

Cited by 14 publications

References 24 publications

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Description of Sarcocystis scandentiborneensis sp. nov. from treeshrews (Tupaia minor, T. tana) in northern Borneo with annotations on the utility of COI and 18S rDNA sequences for species delineation

First molecular characterization of Sarcocystis miescheriana in wild boars (Sus scrofa) from Latvia

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