Sarcocystis species identification in the moose (Alces alces) from the Baltic States

Prakas, Petras; Kirillova, Viktorija; Calero‐Bernal, Rafael; Kirjušina, Muza; Rudaitytė-Lukošienė, Eglė; Habela, M.; Gavarāne, Inese; Butkauskas, Dalius

doi:10.1007/s00436-019-06291-0

Cited by 19 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sarcocystis hjorti was also reported from red deer in Lithuania ( Prakas and Butkauskas, 2012 ) and Spain ( Gjerde et al, 2017b ), suggesting a widespread distribution in Europe. This species uses red deer and moose ( Alces ) as IH ( Gjerde, 2014b ; Prakas et al, 2019 ), and it was shown experimentally, that red foxes and arctic foxes ( Vulpes lagopus ) could act as DH of S. hjorti isolated from moose ( Dahlgren and Gjerde, 2010b ). In the present study, this parasite was also detected in two red foxes (Fox 6 and 46) from the same region, confirming that the red fox is a natural DH of S. hjorti .…”

Section: Discussionmentioning

confidence: 99%

“…This Sarcocystis species uses red deer, moose ( Gjerde, 2014b ; Prakas et al, 2019 ) and sika deer ( Irie et al, 2017 Rudaitytė-Lukošienė et al, 2018 ) as IH and corvid birds as DH. So far, S. ovalis has been only detected in red deer from Norway ( Dahlgren and Gjerde, 2010a ; Gjerde, 2013 ), moose from Norway, Canada ( Dahlgren and Gjerde, 2008 ) and Lithuania ( Prakas et al, 2019 ) and sika deer from Japan ( Irie et al, 2017 ) and Lithuania ( Rudaitytė-Lukošienė et al, 2018 ), and this represents the first record of S. ovalis in Central Europe. The European magpie ( Pica ) and the Japanese jungle crow ( Corvus macrorhynchos ) have been confirmed as definitive hosts for S. ovalis ( Gjerde and Dahlgren, 2010 ; Irie et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…Cunters), sequences with 99.2–100% identity with GenBank® entries of S. linearis derived from red deer (KY973371, KY973372) ( Gjerde et al, 2017b ) and roe deer (MN334301) ( Rudaitytė-Lukošienė et al, 2020 ) were revealed by cloning. This recently described Sarcocystis species uses red deer ( Gjerde et al, 2017b ), roe deer ( Capreolus ) ( Gjerde et al, 2017a ; Rudaitytė-Lukošienė et al, 2020 ) and moose ( Prakas et al, 2019 ) as IH. Its DH was still not described, but based on its phylogenetic position, canids were suspected to play this role ( Gjerde et al, 2017a ).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Basso

Rojas

Buob

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

Red deer ( Cervus elaphus ) carcasses showing grey-greenish discolouration have been increasingly observed in the canton of Grisons, Switzerland. We investigated whether Sarcocystis infections were associated with this pathology, and whether wild and domestic canids were involved in their transmission. Meat from affected red deer ( n = 26), faeces and intestines from red foxes ( Vulpes vulpes ) ( n = 126), and faeces from hunting dogs ( n = 12) from the region, were analysed. Eosinophilic myositis and/or fasciitis were diagnosed in 69% of the deer, and sarcocysts were observed in 89% of the animals. Molecular typing targeting a ~700bp variable region of the 18S rRNA gene revealed Sarcocystis hjorti in 73%, S. venatoria/S. iberica in 54%, S. linearis/S. taeniata in 12%, S. pilosa in 8% and S. ovalis in 4% of the deer samples. No inflammatory changes were observed in red deer carcasses with normal appearance ( n = 8); however, sarcocysts were observed in one sample, and S. hjorti , S. venatoria/S. iberica or S. silva DNA was detected in five samples. Sarcocystis oocysts/sporocysts were observed in 11/106 faecal and 6/20 intestinal fox samples, and in 2/12 canine samples. Sarcocystis tenella ( n = 8), S. hjorti ( n = 2), S. gracilis ( n = 2), and S. miescheriana ( n = 1) were identified in foxes, and S. gracilis ( n = 2), S. capreolicanis ( n = 1) and S. linearis/S. taeniata ( n = 1) in dogs. This study provides first molecular evidence of S. pilosa and S. silva infection in red deer and S. linearis/S. taeniata in dogs and represents the first record of S. ovalis transmitted by corvids in Central Europe. Although Sarcocystis species infecting red deer are not regarded as zoonotic, the affected carcasses can be declared as unfit for human consumption due to the extensive pathological changes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Basso

Rojas

Buob

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

show abstract

“…ovalis has previously been identified in sika deer in Lithuania (Rudaitytė-Lukošienė et al, 2018), but the species was originally reported from moose from Norway and Canada (Dahlgren and Gjerde, 2008) and then from red deer in Norway (Dahlgren and Gjerde, 2010). Recently, S. ovalis was also reported from moose in Lithuania (Prakas et al, 2019).…”

Section: Discussionmentioning

confidence: 98%

“…The species S. taeniata was first described from moose in Canada (Gjerde, 2014b), but has later been found in farmed sika deer in Lithuania (Prakas et al, 2016). The species S. linearis was first described from roe deer in Italy (Gjerde et al, 2017a), but has later been found in red deer in Spain (Gjerde et al, 2017b) and in moose in Lithuania (Prakas et al, 2019). By light microscopy, sarcocysts of S .…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular characteristics of seven Sarcocystis species from sika deer (Cervus nippon centralis) in Japan, including three new species

Abe

Matsuo

Moribe

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

Samples of diaphragm were collected from 53 sika deer from Gifu Prefecture, Japan; 220 sarcocysts were isolated, examined in wet mounts and classified according to their cyst wall protrusions. The sarcocysts were then examined molecularly in order to assign them to different species. All but 11 of the 220 sarcocysts were initially identified by means of a multiplex PCR assay targeting cox1 of five species, whereas the remaining 11 sarcocysts were identified by standard PCR and sequencing. DNA from selected sarcocysts was used for PCR amplification and sequencing of cox1 (59 sequences) and 18S rDNA (23 sequences). The 220 sarcocysts comprised seven major cox1 sequence types or species. Types 4 and 7 were assigned to the known species Sarcocystis pilosa and Sarcocystis ovalis, whereas types 1, 3 and 5 were considered to represent three new species, for which the names Sarcocystis japonica, Sarcocystis matsuoae and Sarcocystis gjerdei have been proposed. Types 2 and 6 were most similar to Sarcocystis tarandi and Sarcocystis taeniata, respectively, but could not be unequivocally assigned to these species. Sarcocysts belonging to S. japonica were macroscopic with fairly thick finger-like protrusions, whereas most sarcocysts of the six other species were microscopic. Sarcocysts of S. cf. tarandi and S. matsuoae were spindle-shaped and possessed thin finger-like cyst-wall protrusions. Sarcocysts of S. pilosa and S. gjerdei had similar hair-like protrusions, whereas those of S. cf. taeniata had a smooth surface. Sarcocysts of S. japonica, S. pilosa, S. cf. tarandi, S. gjerdei, S. matsuoae, S. cf. taeniata and S. ovalis were found in 50 (94.3%), 29 (54.7%), 22 (41.5%), 10 (18.9%), 8 (15.1%), 6 (11.3%) and 1 (1.9%) of the 53 sika deer examined, respectively. An improved multiplex PCR assay targeting cox1 was developed, through which the seven Sarcocystis spp. found in the present study could be identified.

show abstract

Molecular characterisation of five Sarcocystis species in domestic sheep (Ovis aries) from Spain

et al. 2019

View full text Add to dashboard Cite

Sarcocystis species identification in the moose (Alces alces) from the Baltic States

Cited by 19 publications

References 26 publications

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Morphological and molecular characteristics of seven Sarcocystis species from sika deer (Cervus nippon centralis) in Japan, including three new species

Molecular characterisation of five Sarcocystis species in domestic sheep (Ovis aries) from Spain

Contact Info

Product

Resources

About