Sarcocystidae in wild birds of southeastern Brazil

Rêgo, Wagner Martins Fontes do; Costa, Júlia Gatti Ladeia; Baraviera, Ramon Castro de Araujo; Pinto, Lorena Velozo; Bessa, Gabriella de Lima; Lopes, Rosálida Estevam Nazar; Silveira, Júlia A.G.; Vítor, Ricardo Wagner Almeida

doi:10.1590/s1984-296120201094

Cited by 7 publications

(9 citation statements)

References 19 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The experimental intermediate host for both Sarcocystis species is Melopsittacus undulatus; however, its natural intermediate host remains unknown (Dubey et al, 2000(Dubey et al, , 2001b. Rêgo et al (2021) evaluated samples of wild free-ranging birds, naturally infected and rescued in the state of Minas Gerais, Brazil, presenting 5/44 (11.4%) samples positive for Sarcocystis spp. using the 18S ribosomal gene.…”

Section: Resultsmentioning

confidence: 99%

“…Recent studies have described Sarcocystis spp. that affect birds, i.e., S. albifronsi, S. anasi (Kutkienė et al, 2012), S. calchasi (Parmentier et al, 2019), S. columbae (Olias et al, 2010), S. falcatula (Konradt et al, 2017;Llano et al, 2022;Rêgo et al, 2021), S. halieti, S. lari (Gjerde et al, 2018), S. lindsayi (Dubey et al, 2001a), S. ramphastosi (Dubey et al, 2008), and S. rileyi (Dubey et al, 2010). The definitive hosts of S. falcatula are Didelphis albiventris (Dubey et al, 2000), D. aurita (Gallo et al, 2018) and D. marsupialis (Dubey et al, 2001b).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detecção molecular de Sarcocystis spp. no músculo peitoral de aves de cativeiro da região sul do Brasil

et al. 2023

View full text Add to dashboard Cite

Estudos sobre doenças de aves silvestres são essenciais no contexto da saúde pública, pois esses animais atuam como sentinelas, permitindo obter informações sobre uma determinada área geográfica. Além disso, as aves são fontes de proteína alimentar para os animais e, portanto, desempenham um papel importante no ciclo de vida do Sarcocystis. Este estudo teve como objetivo identificar Sarcocystis spp. nos músculos do peito de aves de cativeiro naturalmente infectadas. Os músculos do peito de 89 aves foram coletados, e o DNA amplificado pela PCR do gene RNA ribossômico 18S para detecção de Sarcocystis spp. Os produtos da PCR foram sequenciados e 5,61% (5/89) amostras apresentaram 100% de similaridade com o Sarcocystis spp. (um Cyanoliseus patagonus, um Psittacula krameri, dois Pyrrhura frontalis e um Ramphastos dicolorus). O grande número de espécies naturalmente infectadas analisadas por métodos moleculares permitiu a detecção de Sarcocystis spp. em diferentes espécies de aves, corroborando a epidemiologia de Sarcocystis spp. Até onde sabemos, este é o primeiro relato especificando a presença de Sarcocystis spp. no músculo do peito de espécies de aves.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detecção molecular de Sarcocystis spp. no músculo peitoral de aves de cativeiro da região sul do Brasil

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The most recent studies on Sarcocystis spp. in birds of prey have focused on the diagnosis of this apicomplexan genus using muscle digestion and subsequent nested PCR [ 9 ] or an immunofluorescence antibody test [ 25 ]. These methods are relatively sensitive to the detection of Sarcocystis spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of Sarcocystis halieti in the muscles of two species of birds of prey from Spain

et al. 2021

View full text Add to dashboard Cite

Background Members of the genus Sarcocystis are protozoan parasites characterized by a prey–predator two-host life-cycle. Sarcocysts are formed in the muscles or central nervous system of the intermediate host (IH), while sporocysts develop in the small intestine of the definitive host (DH). Various birds of prey have been confirmed to be DH for Sarcocystis spp. Three Sarcocystis species, S. wobeseri, S. halieti and S. falcatula, have been identified in the muscles of birds of prey, of which the latter are known to be pathogenic and can cause encephalitis in various birds. The aim of this study was to identify Sarcocystis spp. in the muscles of birds of prey from Spain. Methods Between 2019 and 2020, muscle tissue samples taken from 59 birds of prey admitted to the Wildlife Recovery Centre in Ilundain (Navarra, Spain) were examined for the presence of Sarcocystis spp. Sarcocysts in fresh squashed samples were morphologically characterized under the light microscope (LM). Sarcocystis spp. were identified by means of 28S ribosomal RNA and internal transcribed spacer 1 sequence analysis. Results Microscopic examination of squashed tissue samples stained with methylene blue revealed the presence of sarcocysts in three of the 59 (5.1%) birds examined. Only one sarcocyst type was observed under the LM. Sarcocysts were thread-like (1050–2160 × 130–158 μm) and had a thin (0.7–1.4 μm) and smooth cyst wall. Septa divided the cysts into compartments filled with banana-shaped (5.9 × 1.7 μm) bradyzoites. On the basis of DNA sequence results, S. halieti was identified in the western marsh harrier (Circus aeruginosus) and the black kite (Milvus migrans) for the first time. Sarcocysts of S. halieti were shorter and wider compared to those observed in the great cormorant (Phalacrocorax carbo) and the herring gull (Larus argentatus). According to current knowledge, S. halieti may infect birds belonging to four different orders: Suliformes, Charadriiformes, Strigiformes and Accipitriformes. Conclusions This is the first report of S. halieti in the western marsh harrier and the black kite as IH. So far, little research has been conducted on birds of prey as IH for Sarcocystis spp. These results indicate that further studies combining morphological, histopathological, and molecular methods are required. Graphical abstract

show abstract

“…Lastly, thin-walled (≤ 1 µm) and thick-walled (2-4 µm) sarcocysts were detected in the muscles of raptors from the south-eastern USA, however, no detailed microscopical examination was performed [8]. The most recent studies on Sarcocystis from birds of prey focused on diagnosis of this apicomplexan genus using muscle digestion and subsequent nested PCR [9] or an immuno uorescence antibody test [26]. The above-mentioned methods are relatively sensitive to the detection of Sarcocystis spp.…”

Section: Discussionmentioning

confidence: 99%

“…Also, there are numerous studies about birds of prey as IH of Sarcocystis spp. [4][5][6][7][8][9][10]. It was recorded that pathogenic S. falcatula may cause encephalitis in the free-ranging great horned owl (Bubo virginianus) [6], the golden eagle (Aquila chrysaetos) and the bald eagle (Haliaeetus leucocephalus) [7].…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Sarcocystis Halieti in Birds of Prey From Spain

Prakas

Bea²,

Juozaiė-Ngugu

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Members of the genus Sarcocystis are protozoan parasites characterized by a prey-predator two-host life cycle. Sarcocysts are formed in muscles or CNS of the intermediate host (IH), while sporocysts develop in the small intestine of the definitive host (DH). Various birds of prey were confirmed to be DH for Sarcocystis spp. By contrast, only two species, S. wobeseri and S. falcatula were identified in the muscles of birds of prey. The latter species is pathogenic and can cause encephalitis in various birds. The aim of the present study was to identify Sarcocystis species in the muscles of birds of prey from Spain. Methods: In the period between 2019 and 2020, muscle tissues of 59 birds collected from Spain were examined for the presence of Sarcocystis spp. Sarcocysts in fresh squashed samples were morphologically characterised under a light microscope (LM). Sarcocystis species were identified by means of 28S rRNA and ITS1 sequence analysis. Results: With the help of methylene blue-staining microscopic sarcocysts were detected in 3/59 (5.1%) birds of prey from Spain. Under LM, one type of sarcocysts was observed. Sarcocysts were thread-like (1050–2160 × 130–158 μm), had a thin (0.7–1.4 μm) and smooth cyst wall. Septa divided the cysts into compartments filled with banana-shaped (5.9 × 1.7 μm) bradyzoites. On the basis of DNA sequence results, S. halieti was identified in the western marsh harrier (Circus aeruginosus) and the black kite (Milvus migrans) for the first time. Sarcocysts of S. halieti detected in the black kite and the western marsh harrier were shorter and wider as compared to those observed in the great cormorant (Phalacrocorax carbo) and the herring gull (Larus argentatus). Hence, S. halieti might infect birds belonging to three different orders, Suliformes, Charadriiformes and Accipitriformes. Conclusions: This is the first report of S. halieti in birds of prey as IH. Due to the inconsistency of research on Sarcocystis spp. from birds of prey, further complex morphological, histopathological, and molecular studies are required.

show abstract

Sarcocystidae in wild birds of southeastern Brazil

Cited by 7 publications

References 19 publications

Detecção molecular de Sarcocystis spp. no músculo peitoral de aves de cativeiro da região sul do Brasil

Detecção molecular de Sarcocystis spp. no músculo peitoral de aves de cativeiro da região sul do Brasil

Molecular identification of Sarcocystis halieti in the muscles of two species of birds of prey from Spain

Molecular Identification of Sarcocystis Halieti in Birds of Prey From Spain

Contact Info

Product

Resources

About