“sarc” Sequence Transcription in Moloney Sarcoma Virus-Transformed Nonproducer Cell Lines

Bondurant, Maurice C.; Ramabhadran, Rajani; Green, Maurice; Wold, William S.M.

doi:10.1128/jvi.29.1.76-82.1979

Cited by 5 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All transformed nonproducer cells were found to express high levels of both viral RNA and p21 transforming protein (at least 10to 30-fold higher than endogenous mouse ras expression) with little variation between cell lines. This lack of variation was somewhat unexpected and contrasts markedly with results of studies on the expression of other retrovirus proviruses (5,17,41). We can envisage two explanations to account for this observation.…”

Section: Discussioncontrasting

confidence: 60%

See 1 more Smart Citation

Expression of Kirsten murine sarcoma virus in transformed nonproducer and revertant NIH/3T3 cells: evidence for cell-mediated resistance to a viral oncogene in phenotypic reversion

Norton¹,

Cook²,

Roberts³

et al. 1984

J Virol

View full text Add to dashboard Cite

Expression of the provirus in a clonally related series of Kirsten murine sarcoma virus-transformed NIH/ 3T3 nonproducer cell lines was examined at both the transcriptional and translational levels. All cells expressed high levels of genome-sized viral RNA with little variation between cell lines despite differences in provirus integration site and copy number. Expression of K-ratis RNA was estimated to be at least 10to 20-fold higher than that of the mouse cellular homolog of the viral transforming gene. Levels of the viruscoded transforming protein, p21, were similarly elevated, with little variation between nonproducer cells. In two revertant cell lines containing a normal provirus and a rescuable transforming gene, no impairment in expression at either the transcriptional or translational level was found. After superinfection with Kirsten murine sarcoma virus, one revertant became more tumorigenic. whereas the other remained nontumorigenic. These results show that cell transformation by Kirsten murine sarcoma virus is invariably associated with elevated expression of the virus-coded oncogene and that one of the revertants is resistant to the action of the viral transforming gene.

show abstract

Section: Discussioncontrasting

confidence: 60%

“…Taking into account the fact that the coding sequence for the K-ras p21 is ca. 0.6 kilobase (40), we calculated that expression of K-ras-containing transcripts from the KiMSV provirus occurs at about KiMSV EXPRESSION AND CELL TRANSFORMATION 441 2 5-…”

Section: Resultsmentioning

confidence: 99%

Expression of Kirsten murine sarcoma virus in transformed nonproducer and revertant NIH/3T3 cells: evidence for cell-mediated resistance to a viral oncogene in phenotypic reversion

Norton¹,

Cook²,

Roberts³

et al. 1984

J Virol

View full text Add to dashboard Cite

show abstract

Sub-genomic RNA in Moloney leukemia virus grown in Iymphoid-derived cell lines consists primarily of homologous viral RNA

et al. 1979

View full text Add to dashboard Cite

Moloney murine leukemia virus (MoMuLV) grown in 2 lymphoid-derived murine cell lines (JLS-V9 and TB) contained 2 size classes of RNA subunits: 2.8 +/- 0.4 x 10(6) (n = 30) and 1.6 +/- 0.1 x 10(6) (n = 15) daltons. Detectable levels of low molecular weight viral RNA (LMW vRNA) were not present in MoMuLV grown in mouse embryo fibroblasts, nor rat cells, nor was it produced by them when they were infected with MoMuLV containing both species of vRNA. DNA probes complementary to both subunits were synthesized separately using purified reverse transcriptase (R.T.) and calf thymus DNA as primer. Polyadenylated LMW vRNA was selected and, by molecular hybridization was found to be completely homologous with the large RNA subunit. This was confirmed using cDNA probes representing defined regions of the genome. The LMW vRNA therefore contains multiple subsets of the viral RNA, which could correspond to multiple deletion mutants possibly generated by an efficient RNA splicing mechanism. In addition, low levels of non-homologous virus-like RNA were detected in MoMuLV grown in TB (2%) AND NIH/3T3 cells (0.4%).

show abstract

Biological and Molecular Biological Characterization of the Virus Progeny from Transformed Clones MuSV-124 and MuSV-349: Evidence for MuLV-specific Nucleotide Sequences in the MoMuSV Size Class of RNA from MoMuSV-124

Dekaban

Ball

Loosmore

et al. 1980

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYThe genetic information of MoMuSV-349 and MoMuSV-I24, two clones of productively transformed TB cells, was distributed between two size classes of RNA (tool. wt. 2-9 × IO 6 and I'9 × I@) in the proportions of 5: 1. Some preparations of MoMuSV-I24 lacked the large RNA. The virions produced by both clones also contained all the nucleotide sequences of Moloney leukaemia virus and the ratio of MuSV:MuLV produced by the two clones differed markedly. The distribution of the sequences specific for Moloney murine leukaemia virus (MoMuLV) between the two size classes of RNA was studied using molecular hybridization to DNA probes complementary to and representative of: (i) the Moloney murine sarcoma virus (MoMuSV) RNA genome (tool. wt. I'9 × t@); (ii) those nucleotide sequences shared by MoMuSV and MoMuLV; (iii) nucleotide sequences specific for MoMuSV; (iv) nucleotide sequences specific for MoMuLV. The only detectable Moloney leukaemia virus-specific nucleotide sequences present in MoMuSV-I24 virions were in the RNA of tool. wt. 1"9 x lO ~, whereas these sequences were detected in the RNA of mol. wt. 2"9x xo 6 isolated from MoMuSV-349 virions. The biological properties of the replicating information in MoMuSV-I24 suggest that, consistent with the small size of RNA, it is defective, whereas MoMuSV-349 produces virions containing an intact MoMuLV genome, competent for replication.

show abstract

“sarc” Sequence Transcription in Moloney Sarcoma Virus-Transformed Nonproducer Cell Lines

Cited by 5 publications

References 29 publications

Expression of Kirsten murine sarcoma virus in transformed nonproducer and revertant NIH/3T3 cells: evidence for cell-mediated resistance to a viral oncogene in phenotypic reversion

Expression of Kirsten murine sarcoma virus in transformed nonproducer and revertant NIH/3T3 cells: evidence for cell-mediated resistance to a viral oncogene in phenotypic reversion

Sub-genomic RNA in Moloney leukemia virus grown in Iymphoid-derived cell lines consists primarily of homologous viral RNA

Biological and Molecular Biological Characterization of the Virus Progeny from Transformed Clones MuSV-124 and MuSV-349: Evidence for MuLV-specific Nucleotide Sequences in the MoMuSV Size Class of RNA from MoMuSV-124

Contact Info

Product

Resources

About