Saponins of Japanese Dioscoreaceae. III

Partition Chromatography Of Steroids

“…Concentrated sulfuric acid (322) and fuming sulfuric acid (12) give characteristic colors or fluorescence with many steroids (462,13,6,281,425,316)8 but the filter paper soon disintegrates. The Liebermann-Burchard reagent (sulfuric acid-acetic anhydride) (298,235,215,316,119,429) is less destructive but gives a fluorescence similar to that of the more convenient 15 per cent phosphoric acid reagent proposed by Neher and Wettstein (282). The latter reagent gives a variety of colors in ultraviolet light with minute amounts of all types of steroids (298,190,230,393,150,316,39).…”

“…Certain generalizations on the effect of configuration on chromatographic mobility were made which may be valuable for predicting the structure of unknown sapogenins. For paper chromatography of the saponins 1-butanolor isoamyl alcohol-acetic acid-water (4:1:5) mixtures have been proposed (429).…”

“…, Narthecium(398), and Japanese Dioscoreaceae(426,428,429,427). Steroidal alkaloids identified by partition chromatography included those from…”