Saponins extracted from Polemonium caeruleum have adjuvant activity in guinea pig intranasal immunization with trivalent influenza antigens

Va, Evseenko; As, Gudymo; Nv, Danilchenko; Gs, Onkhonova; Lт, Vu; Ab, Ryzhikov

doi:10.15761/fdccr.1000142

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Frontiers Drug Chemistry Clinical Res

2020

DOI: 10.15761/fdccr.1000142

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Saponins extracted from Polemonium caeruleum have adjuvant activity in guinea pig intranasal immunization with trivalent influenza antigens

Evseenko Va¹,

Gudymo As²,

Danilchenko Nv³

et al.

Abstract: Saponins extracted from Polemonium caeruleum were studied as intranasal adjuvant by guinea pig intranasal immunization with trivalent influenza antigens. Sera titers of the animals immunized intranasally and control animals immunized intramuscularly were measured in HI and ELISA. On day 21 reciprocal GMT HI titers in group dually immunized intranasally were 201/64/31 against H1N1pdm09/H3N2/B components accordingly. Data obtained in ELISA shows that there was no statistically significant (P=0.95) differences in… Show more

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2022

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Development and laboratory production of virus-like immune-stimulating complexes based on saponins and evaluation of their adjuvant potential using mice immunisation with influenza antigens

Evseenko

Gudymo

Danilchenko

et al. 2022

Biological Products. Prevention, Diagnosis, Treatment

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The COVID-19 pandemic has exacerbated the public’s need for effective vaccines. Consequently, significant financial support has been provided to developers of a number of innovative vaccines, including the vaccines with saponin-based adjuvants. In 2021, the World Health Organisation recommended Mosquirix, the first malaria vaccine, which contains a saponin adjuvant. An anti-covid vaccine by Novavax is in the approval phase. A promising approach to vaccine development is presented by the use of virus-like immune-stimulating complexes (ISCOMs) containing saponins and by the creation of combinations of ISCOMs with antigens. The aim of the study was to develop, produce and characterise virus-like immune-stimulating complexes based on saponins of Quillaja saponaria, as well as similar saponins of Russian-sourced Polemonium caeruleum. Materials and methods: The ISCOM adjuvants, Matrix-BQ and Matrix-BP, were produced using liquid chromatography and examined using electron microscopy. Balb/c mice were immunised intraperitoneally and intramuscularly with ISCOM-antigen preparations. Afterwards, the immunised animals were challenged with the influenza virus strain, A/California/4/2009(H1N1)pdm09, adapted and lethal to mice. The serum samples were examined using haemagglutination inhibition (HI) tests. Results: The authors produced the ISCOMs containing saponins of Quillaja saponaria and Polemonium caeruleum. After one intramuscular injection of either of the ISCOM-antigen preparations with 1 µg of each of A/Brisbane/02/2018 (H1N1) pdm09, A/Kansas/14/2017 (H3N2), and B/Phuket/3073/2013 haemagglutinin antigens (HAs), HI tests detected serum antibody titres to the corresponding antigens of ≥1:40. Two intramuscular injections of the ISCOM-antigen preparation containing 50 ng of each of the HAs and Matrix-BQ resulted in a protective response. In some animals, two intraperitoneal injections of ISCOM-antigen preparations resulted in the maximum antibody titre to the A/Kansas/14/2017 (H3N2) vaccine strain of 1:20,480. Two intramuscular injections of a test preparation containing 5 µg, 1 µg, 200 ng, or 50 ng of each of the HAs and Matrix-BQ or a control preparation containing 5 µg, 1 µg, or 200 ng of each of the HAs (commercially available vaccines) to the mice that were afterwards infected with the lethal influenza strain protected the experimental animals from death. Conclusions: The ISCOM-based preparations had high immunostimulatory activity in the mouse-model study. The presented results indicate the potential of further studies of ISCOM-based preparations in terms of both vaccine and immunotherapeutic development.

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Development and laboratory production of virus-like immune-stimulating complexes based on saponins and evaluation of their adjuvant potential using mice immunisation with influenza antigens

Evseenko

Gudymo

Danilchenko

et al. 2022

Biological Products. Prevention, Diagnosis, Treatment

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show abstract

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Saponins extracted from Polemonium caeruleum have adjuvant activity in guinea pig intranasal immunization with trivalent influenza antigens

Cited by 1 publication

References 13 publications

Development and laboratory production of virus-like immune-stimulating complexes based on saponins and evaluation of their adjuvant potential using mice immunisation with influenza antigens

Development and laboratory production of virus-like immune-stimulating complexes based on saponins and evaluation of their adjuvant potential using mice immunisation with influenza antigens

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