Saponin-enhanced biomass accumulation and demulsification capability of the demulsifying bacteria Alcaligenes sp. S-XJ-1

Huang, Xiangfeng; Zhang, Yuyan; Wei, Yuezhou; Liu, Jia; Liu, Lu; Peng, Kaiming

doi:10.1039/c6ra02237e

Cited by 6 publications

(2 citation statements)

References 40 publications

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“…The extract in the same concentration generated an increase inthis parameter in the case of A. calcoaceticus . Those results are contrary to previous studies which have suggested that saponins increase cell membrane permeability and CSH while decreasing Zeta potential [38]. This inconsistency may be due to the complex composition of the ivy extract and the resultant impact on all of its components.…”

Section: Resultscontrasting

confidence: 97%

Environmental Aspects of the Use of Hedera helix Extract in Bioremediation Process

et al. 2019

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This paper analyzes the impact of saponins from English ivy leaves on the properties of environmental bacterial strains and hydrocarbon degradation ability. For this purpose, two bacterial strains, Raoultella ornitinolytica M03 and Acinetobacter calcoaceticus M1B, have been used in toluene, 4-chlorotoluene, and α,α,α-trifluorotoluene biodegradation supported by Hedera helix extract. Moreover, theeffects of ivy exposition on cell properties and extract toxicity were investigated. The extract was found to cause minor differences in cell surface hydrophobicity, membrane permeability, and Zeta potential, although it adhered to the cell surface. Acinetobacter calcoaceticus M1B was more affected by the ivy extract; thus, the cells were more metabolically active and degraded saponins at greater amounts. Although the extract influenced positively the cells’ viability in the presence of hydrocarbons, it could have been used by the bacteria as a carbon source, thus slowing down hydrocarbon degradation. These results show that the use of ivy saponins for hydrocarbon remediation is environmentally acceptable but should be carefully analyzed to assess the efficiency of the selected saponins-rich extract in combination with selected bacterial strains.

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Section: Resultscontrasting

confidence: 97%

Environmental Aspects of the Use of Hedera helix Extract in Bioremediation Process

et al. 2019

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“…It can be crucial for effective biodegradation of hydrophobic pollutants because the limited permeability of the cell envelope can also limit the transmembrane transport, which may be the rate-limiting step in hydrocarbons utilization. Moreover, the surfactants, as permeabilizing agents, can increase the transmembrane diffusion of the pollutants [40]. Taking into account relatively high toxicity of A. hippocastanum and V. nigrum towards A. calcoaceticus M1B cells, as well as the positive effect of S. muccorosi and rhamnolipids on all analyzed strains, the two later extracts were used for further analysis, together with the synthetic surfactant Triton X-100.…”

Section: Discussionmentioning

confidence: 99%

Surfactant addition in diesel oil degradation – how can it help the microbes?

Zdarta

Smułek

Pacholak

et al. 2020

J Environ Health Sci Engineer

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Purpose Despite wide research on bioremediation of hydrocarbon-contaminated soil, the mechanisms of surfactant-enhanced bioavailability of the contaminants are still unclear. The presented study was focused on the in-depth description of relationships between hydrocarbons, bacteria, and surfactants. In order to that, the biodegradation experiments and cell viability measurements were conducted, and the properties of cell surface were characterized. Methods MTT assay was employed to measure plant extracts toxicity to microbes. Then, membrane permeability changes were evaluated, followed by diesel oil biodegradation in the presence of surfactants measurements by GCxGC-TOFMS and PCR-RAPD analysis. Results Our study undoubtedly proves that different surfactants promote assimilation of different groups of hydrocarbons and modify cell surface properties in different ways. Increased biodegradation of diesel oil was observed when cultures with Acinetobacter calcoaceticus M1B were supplemented with Saponaria officinalis and Verbascum nigrum extracts. Interestingly, these surfactants exhibit different influences on cell surface properties and their viability in contrast to the other surfactants. Moreover, the preliminary analyses have shown changes in the genome caused by exposure to surfactants. Conclusions The results indicated that the benefits of surfactant use may be related to deep modification at the omics level, not only that of cell surface properties and confirms the complexity of the interactions between bacterial cells, pollutants and surfactants.

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Chemically modified surface functional groups of Alcaligenes sp. S-XJ-1 to enhance its demulsifying capability

Zhang

Liu

Huang

et al. 2017

Appl Microbiol Biotechnol

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Cell-surface functional groups (amino, carboxyl, hydroxyl, as well as phosphate) were chemically modified in various ways to enhance the demulsification capability of the demulsifying bacteria Alcaligenes sp. S-XJ-1. Results demonstrated that the demulsifying activity was significantly inhibited by amino enrichment with cetyl trimethyl ammonium bromide, amino methylation, hydroxyl acetylation, and phosphate esterification, but was gradually promoted by carboxyl blocking with increasing the extents of esterification. Compared with the raw biomass, an optimal esterification of carboxyl moieties enhanced the demulsification ratio by 26.5% and shortened the emulsion half-life from 24 to 8.8 h. The demulsification boost was found to be dominated by strengthened hydrophobicity (from 53° to 74°) and weakened electronegativity (from -34.6 to -4.3 mV at pH 7.0) of the cell surface, allowing the rapid dispersion and adsorption of cells onto the oil-water interface. The chemical modification of the functional groups on the biomass surface is a promising tool for the creation of a high-performance bacterial demulsifier.

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Saponin-enhanced biomass accumulation and demulsification capability of the demulsifying bacteria Alcaligenes sp. S-XJ-1

Abstract: Saponin significantly enhanced biomass accumulation and demulsification capability of the demulsifying bacteria.

Cited by 6 publications

References 40 publications

Environmental Aspects of the Use of Hedera helix Extract in Bioremediation Process

Environmental Aspects of the Use of Hedera helix Extract in Bioremediation Process

Surfactant addition in diesel oil degradation – how can it help the microbes?

Chemically modified surface functional groups of Alcaligenes sp. S-XJ-1 to enhance its demulsifying capability

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