Saponin-Based Nanoemulsification Improves the Antioxidant Properties of Vitamin A and E in AML-12 Cells

Choudhry, Qaisra Naheed; Kim, Mi Jeong; Kim, Tae Kyun; Pan, Jeong Hoon; Kim, Jun Ho; Park, Sung Jin; Lee, Jin Hyup; Kim, Young Jun

doi:10.3390/ijms17091406

Cited by 18 publications

(11 citation statements)

References 45 publications

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“…Liposomes for parenteral administration can also ameliorate the stability of verbascoside by preventing its hydrolysis [ 9 ]. Nevertheless, nanoemulsions may increase the stability of natural compounds and encapsulation improves the antioxidant activity of APIs [ 19 ]. It is worth noting that our PL-SNEDDS compositions potentiated the free radical scavenging activity of P. lanceolata extract compared to positive control (non-encapsulated P. lanceolata extract).…”

Section: Discussionmentioning

confidence: 99%

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Self-Nanoemulsifying Drug Delivery Systems Containing Plantago lanceolata—An Assessment of Their Antioxidant and Antiinflammatory Effects

et al. 2017

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The most important components of Plantago lanceolata L. leaves are catalpol, aucubin, and acteoside (=verbascoside). These bioactive compounds possess different pharmacological effects: anti-inflammatory, antioxidant, antineoplastic, and hepatoprotective. The aim of this study was to protect Plantago lanceolata extract from hydrolysis and to improve its antioxidant effect using self-nano-emulsifying drug delivery systems (SNEDDS). Eight SNEDDS compositions were prepared, and their physical properties, in vitro cytotoxicity, and in vivo AST/ALT values were investigated. MTT cell viability assay was performed on Caco-2 cells. The well-diluted samples (200 to 1000-fold dilutions) proved to be non-cytotoxic. The acute administration of PL-SNEDDS compositions resulted in minor changes in hepatic markers (AST, ALT), except for compositions 4 and 8 due to their high Transcutol contents (80%). The non-toxic compositions showed a significant increase in free radical scavenger activity measured by the DPPH test compared to the blank SNEDDS. An indirect dissolution test was performed, based on the result of the DPPH antioxidant assay; the dissolution profiles of Plantago lancolata extract were statistically different from each SNEDDS. The anti-inflammatory effect of PL-SNEDDS compositions was confirmed by the ear inflammation test. For the complete examination period, all compositions decreased ear edema as compared to the positive (untreated) control. It can be concluded that PL-SNEDDS compositions could be used to deliver active natural compounds in a stable, efficient, and safe manner.

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Section: Discussionmentioning

confidence: 99%

“…DPPH reduction assay was an efficient and rapid method to screen the scavenger activity of PL-SNEDDS samples in vitro [ 20 ]. DPPH reaction is predictive but not sufficient to certify the therapeutic potential of P. lanceolata extract [ 19 ]. Therefore, ear inflammation test was carried out.…”

Section: Discussionmentioning

confidence: 99%

Self-Nanoemulsifying Drug Delivery Systems Containing Plantago lanceolata—An Assessment of Their Antioxidant and Antiinflammatory Effects

et al. 2017

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“…The coarse emulsion was then processed two times in a ultra-high-pressure homogenizer at 200 MPa. The resulting nanoemulsions (droplet size < 300 nm) were used for preparation of retinyl acetate micelles for in vitro evaluation of their biological functions [ 89 ]. Tanglao et al (2019) also used whey proteins (polymerized) to encapsulate vitamin A in virgin coconut O/W emulsions.…”

Section: Encapsulation Of Lipid-soluble Bioactive Compounds By Nanmentioning

confidence: 99%

“…The authors demonstrated that vitamins were more effective to suppress lipid peroxidation, protein carbonylation, and DNA damage reactions in emulsified state compared to their non-emulsified form. In another recent study, nanoemulsions containing Tween 80 in combination with various encapsulating agents (Capsul, maltodextrin, sodium caseinate) were successfully used to encapsulate vitamins A and E for application in extruded feed products [ 89 ].…”

Section: Encapsulation Of Lipid-soluble Bioactive Compounds By Nanmentioning

confidence: 99%

Encapsulation of Lipid-Soluble Bioactives by Nanoemulsions

et al. 2020

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Lipid-soluble bioactives are important nutrients in foods. However, their addition in food formulations, is often limited by limited solubility and high tendency for oxidation. Lipid-soluble bioactives, such as vitamins A, E, D and K, carotenoids, polyunsaturated fatty acids (PUFA) and essential oils are generally dispersed in water-based solutions by homogenization. Among the different homogenization technologies available, nanoemulsions are one of the most promising. Accordingly, this review aims to summarize the most recent advances in nanoemulsion technology for the encapsulation of lipid-soluble bioactives. Modern approaches for producing nanoemulsion systems will be discussed. In addition, the challenges on the encapsulation of common food ingredients, including the physical and chemical stability of the nanoemulsion systems, will be also critically examined.

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“…Meanwhile, ROS may lead to a series of diseases, such as diabetes, hypertension, cancer and atherosclerotic cardiovascular disease (Finkel et al , 2000; Memarpoor-Yazdi et al , 2012 ). Recently, more and more researchers had focused on extraction of novel, safe and natural antioxidants, for instance, carotenoids and vitamin A ( Choudhry et al , 2016 ; Korekar et al , 2014; Maadane et al , 2015 ; Páramo et al , 2012). Such antioxidants are mainly obtained from vegetables, fruits, foods ( Geetha et al , 2002 ; Podsedk, 2007 ), and are regarded as the second antioxidant defense line in living organisms ( Yigit et al , 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Purification and Identification of a Natural Antioxidant Protein from Fertilized Eggs

Yang¹,

Wang²,

Wang³

et al. 2017

Korean Journal for Food Science of Animal Resources

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Fertilized hen eggs are rich in a variety of bioactive ingredients. In this study, we aimed to obtain an antioxidant protein from fertilized eggs and the radical scavenging abilities on 1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (•OH), superoxide anion (O2−•) were used to evaluate the antioxidant activity of the purified protein. During 20 d of incubation, the radical scavenging ability of protein extracted from fertilized eggs exhibited significantly differences and the protein on day 16 showed higher antioxidant capacity. Based on this, the antioxidant protein of the samples on day 16 were isolated for the follow-up study. With a molecular weight 43.22 kDa, the antioxidant protein was purified by Diethylaminoethyl cellulose −52 (DEAE-52) column and Sephadex G-100. The LC-MS analysis showed that the purified protein molecular weight was 43.22 kDa, named D2-S. The sequence of amino acids was highly similar to ovalbumin and the coverage reached to 84%. The purified protein showed a radical scavenging rate of 52.34±3.27% on DPPH and 63.49±0.25% on •OH, respectively. Furthermore, the C-terminal amino acid sequence was NAVLFFGRCVSP, which was consistent with the sequence of ovabumin. These results here indicated that purified protein may be a potential resource as a natural antioxidant.

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Saponin-Based Nanoemulsification Improves the Antioxidant Properties of Vitamin A and E in AML-12 Cells

Cited by 18 publications

References 45 publications

Self-Nanoemulsifying Drug Delivery Systems Containing Plantago lanceolata—An Assessment of Their Antioxidant and Antiinflammatory Effects

Self-Nanoemulsifying Drug Delivery Systems Containing Plantago lanceolata—An Assessment of Their Antioxidant and Antiinflammatory Effects

Encapsulation of Lipid-Soluble Bioactives by Nanoemulsions

Purification and Identification of a Natural Antioxidant Protein from Fertilized Eggs

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