Saponin B, a novel cytostatic compound purified from Anemone taipaiensis, induces apoptosis in a human glioblastoma cell line

Wang, Yuangang; Tang, Hai‐Feng; Zhang, Yun; Li, Juan; Li, Bo; Gao, Zhenhui; Wang, Xiaoyang; Cheng, Guang; Zhou, Fei

doi:10.3892/ijmm.2013.1500

Cited by 15 publications

(18 citation statements)

References 41 publications

(32 reference statements)

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“…Saponin B blocked the cell cycle at the S phase 31 . Saponin B induced chromatin condensation of U87MG GBM cells and led to the formation of apoptotic bodies.…”

Section: Bioactivitiesmentioning

confidence: 95%

“…Raddeanin A increased BAX expression, reduced BCL-2, BCL-xL and survivin expression, and significantly activated caspase-3, caspase-8, caspase-9 and poly-ADP ribose polymerase (PARP) 25 . Saponins B, 1, and 6 of A. taipaiensis exhibit significant anticancer activity against human leukemia, glioblastoma multiforme (GBM), and HCC26, 27, 28, 29, 30, 31. Saponin 1 caused characteristic apoptotic morphological changes in GBM cells 26 , which was confirmed by DNA ladder electrophoresis and flow cytometry.…”

Section: Bioactivitiesmentioning

confidence: 99%

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Anemone medicinal plants: ethnopharmacology, phytochemistry and biology

Hao

Xiao

2017

Acta Pharmaceutica Sinica B

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The Ranunculaceae genus Anemone (order Ranunculales), comprising more than 150 species, mostly herbs, has long been used in folk medicine and worldwide ethnomedicine. Various medicinal compounds have been found in Anemone plants, especially triterpenoid saponins, some of which have shown anti-cancer activities. Some Anemone compounds and extracts display immunomodulatory, anti-inflammatory, antioxidant, and antimicrobial activities. More than 50 species have ethnopharmacological uses, which provide clues for modern drug discovery. Anemone compounds exert anticancer and other bioactivities via multiple pathways. However, a comprehensive review of the Anemone medicinal resources is lacking. We here summarize the ethnomedical knowledge and recent progress on the chemical and pharmacological diversity of Anemone medicinal plants, as well as the emerging molecular mechanisms and functions of these medicinal compounds. The phylogenetic relationships of Anemone species were reconstructed based on nuclear ITS and chloroplast markers. The molecular phylogeny is largely congruent with the morphology-based classification. Commonly used medicinal herbs are distributed in each subgenus and section, and chemical and biological studies of more unexplored taxa are warranted. Gene expression profiling and relevant “omics” platforms could reveal differential effects of phytometabolites. Genomics, transcriptomics, proteomics, and metabolomics should be highlighted in deciphering novel therapeutic mechanisms and utilities of Anemone phytometabolites.

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“…Saponin B blocked the cell cycle at the S phase 31 . Saponin B induced chromatin condensation of U87MG GBM cells and led to the formation of apoptotic bodies.…”

Section: Bioactivitiesmentioning

confidence: 95%

Section: Bioactivitiesmentioning

confidence: 99%

Anemone medicinal plants: ethnopharmacology, phytochemistry and biology

Hao

Xiao

2017

Acta Pharmaceutica Sinica B

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“…In particular, numerous saponins have recently been reported to inhibit glioma cell proliferation in vitro (19)(20)(21)(22) and suppress glioblastoma progression in vivo (21). Our previous studies demonstrated that saponin B and saponin 1 derived from A. taipaiensis exhibited significant in vitro and in vivo antiglioma activity (10,11). In addition, our previous preliminary structure-activity relationship studies demonstrated that the cytotoxicity of these A. taipaiensis-derived saponins is markedly affected by the length of their oligosaccharide chain at C-3.…”

Section: Discussionmentioning

confidence: 98%

“…In particular, saponins derived from the rhizomes of Anemone taipaiensis (Ranunculaceae) exhibit potent cytotoxicity toward the human hepatocellular carcinoma cell line HepG2 and the human promyelocytic leukemia cell line HL-60 (9). Furthermore, our previous studies demonstrated that saponin 1 and saponin B, derived from A. taipaiensis, effectively inhibited brain tumor progression (10,11). Following these results, the present study aimed to determine the potential antiglioma activities of other saponins derived from A. taipaiensis.…”

Section: Introductionmentioning

confidence: 89%

Saponin 6 derived from Anemone taipaiensis induces U87 human malignant glioblastoma cell apoptosis via regulation of Fas and Bcl-2 family proteins

Tang

et al. 2016

Molecular Medicine Reports

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Glioblastoma multiforme (GBM) is the most common and aggressive type of brain tumor, and is associated with a poor prognosis. Saponin 6, derived from Anemone taipaiensis, exerts potent cytotoxic effects against the human hepatocellular carcinoma HepG2 cell line and the human promyelocytic leukemia HL‑60 cell line; however, the effects of saponin 6 on glioblastoma remain unknown. The present study aimed to evaluate the effects of saponin 6 on human U87 malignant glioblastoma (U87 MG) cells. The current study revealed that saponin 6 induced U87 MG cell death in a dose‑ and time‑dependent manner, with a half maximal inhibitory concentration (IC50) value of 2.83 µM after treatment for 48 h. However, saponin 6 was needed to be used at a lesser potency in HT‑22 cells, with an IC50 value of 6.24 µM. Cell apoptosis was assessed by flow cytometry using Annexin V‑fluorescein isothiocyanate/propidium iodide double staining. DNA fragmentation and alterations in nuclear morphology were examined by terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling and transmission electron microscopy, respectively. The present study demonstrated that treatment with saponin 6 induced cell apoptosis in U87 MG cells, and resulted in DNA fragmentation and nuclear morphological alterations typical of apoptosis. In addition, flow cytometric analysis revealed that saponin 6 was able to induce cell cycle arrest. The present study also demonstrated that saponin 6‑induced apoptosis of U87 MG cells was attributed to increases in the protein expression levels of Fas, Fas ligand, and cleaved caspase‑3, ‑8 and ‑9, and decreases in the levels of B‑cell lymphoma 2. The current study indicated that saponin 6 may exhibit selective cytotoxicity toward U87 MG cells by activating apoptosis via the extrinsic and intrinsic pathways. Therefore, saponin 6 derived from A. taipaiensis may possess therapeutic potential for the treatment of GBM.

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“…In the present study, we investigated the influence of CRM197 and shRNA-VCAM-1 on the activation of caspase-3, 8, and 9, which contribute most in the apoptosis of glioma cells (Wang et al, 2013b;Guo et al, 2014;Xie et al, 2014). Caspase-3 is a common proteolytic enzyme involved in both the extrinsic and intrinsic apoptotic pathways (Wang et al, 2013c). The expression of pro-caspase-3 was decreased and ratio of cleaved caspase-3 was elevated by treatments of CRM197 and shRNA-VCAM-1 alone or in combination, indicating that both agents increased the activation of caspase-3 to induce apoptosis.…”

Section: Discussionmentioning

confidence: 99%

CRM197 in Combination With shRNA Interference of VCAM-1 Displays Enhanced Inhibitory Effects on Human Glioblastoma Cells

Lin

Wang

et al. 2015

J. Cell. Physiol.

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CRM197 is a naturally nontoxic diphtheria toxin mutant that binds and inhibits heparin-binding epidermal growth factor-like growth factor. CRM197 serves as carrier protein for vaccine and other therapeutic agents. CRM197 also inhibits the growth, migration, invasion and induces apoptosis in various tumors. Vascular cell adhesion molecule-1 (VCAM-1) is an important cell surface adhesion molecule associated with malignancy of gliomas. In this work, we aimed to investigate the role and mechanism of CRM197 combined with shRNA interference of VCAM-1 (shRNA-VCAM-1) on the migration, invasion and apoptosis of glioblastoma cells. U87 and U251 human glioblastoma cells were treated with CRM197 (10 μg/ml) and shRNA interfering technology was employed to silence VCAM-1 expression. Cell viability, migration, invasiveness and apoptosis were assessed with CCK8, Transwell and Annexin V-PE/7-AAD staining. Activation of cleaved caspase-3, 8 and 9, activity of matrix metalloproteinase-2/9 (MMP-2/9) and expression of phosphorylated Akt (p-Akt) were also checked. Results showed that CRM197 and shRNA-VCAM-1 not only significantly inhibited the cell proliferation, migration, invasion, but also promoted the apoptosis of U87 and

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Saponin B, a novel cytostatic compound purified from Anemone taipaiensis, induces apoptosis in a human glioblastoma cell line

Cited by 15 publications

References 41 publications

Anemone medicinal plants: ethnopharmacology, phytochemistry and biology

Anemone medicinal plants: ethnopharmacology, phytochemistry and biology

Saponin 6 derived from Anemone taipaiensis induces U87 human malignant glioblastoma cell apoptosis via regulation of Fas and Bcl-2 family proteins

CRM197 in Combination With shRNA Interference of VCAM-1 Displays Enhanced Inhibitory Effects on Human Glioblastoma Cells

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