Saponin attenuates diesel exhaust particle (DEP)-induced MUC5AC expression and pro-inflammatory cytokine upregulation via TLR4/TRIF/NF-κB signaling pathway in airway epithelium and ovalbumin (OVA)-sensitized mice

Jo, Sooyeon; Na, Hyung Gyun; Choi, Yoon Seok; Bae, Chang Hoon; Song, Si-Youn; Kim, Yong-Dae

doi:10.1016/j.jgr.2022.03.009

Cited by 6 publications

(6 citation statements)

References 26 publications

(27 reference statements)

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“…Additionally, the STAT6 and NF-κB pathways are reported to be associated with the production of mucin. 39,40 Activated STAT6 is mainly involved in IL-4/IL-13 responsiveness, 41−43 and knockdown of STAT6 provides protection from IL-13-stimulated mucus production in vivo. Furthermore, NF-κB/p65 was reported as an inhibitor of mucus production.…”

Section: Discussionmentioning

confidence: 99%

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Wogonoside Ameliorates Airway Inflammation and Mucus Hypersecretion via NF-κB/STAT6 Signaling in Ovalbumin-Induced Murine Acute Asthma

Yu,

Cai,

et al. 2024

J. Agric. Food Chem.

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Asthma is recognized as a chronic respiratory illness characterized by airway inflammation and airway hyperresponsiveness. Wogonoside, a flavonoid glycoside, is reported to significantly alleviate the inflammation response and oxidative stress. Herein, this study aimed to investigate the therapeutic effect and underlying mechanism of wogonoside on airway inflammation and mucus hypersecretion in a murine asthma model and in human bronchial epithelial cells (16HBE). BALB/c mice were sensitized and challenged with ovalbumin (OVA). Pulmonary function and the number of cells in the bronchoalveolar lavage fluid (BALF) were examined. Pathological changes in lung tissue in each group were evaluated via hematoxylin and eosin and periodic acid–Schiff staining, and changes in levels of cytokines in BALF and of immunoglobulin E in serum were determined via an enzyme-linked immunosorbent assay. The expression of relevant genes in lung tissue was analyzed via real-time PCR. Western blotting and immunofluorescence were employed to detect the expression of relevant proteins in lung tissue and 16HBE cells. Treatment with 10 and 20 mg/kg wogonoside significantly attenuated the OVA-induced increase of inflammatory cell infiltration, mucus secretion, and goblet cell percentage and improved pulmonary function. Wogonoside treatment reduced the level of T-helper 2 cytokines including interleukin (IL)-4, IL-5, and IL-13 in BALF and of IgE in serum and decreased the mRNA levels of cytokines (IL-4, IL-5, IL-6, IL-13, and IL-1β and tumor necrosis factor-α), chemokines (CCL-2, CCL-11, and CCL-24), and mucoproteins (MUC5AC, MUC5B, and GOB5) in lung tissues. The expression of MUC5AC and the phosphorylation of STAT6 and NF-κB p65 in lung tissues and 16HBE cells were significantly downregulated after wogonoside treatment. Thus, wogonoside treatment may effectively decrease airway inflammation, airway remodeling, and mucus hypersecretion via blocking NF-κB/STAT6 activation.

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Section: Discussionmentioning

confidence: 99%

“…The decreased levels of cytokines induced by wogonoside treatment helped reduce the overproduction of mucus by epithelial cells. Additionally, the STAT6 and NF-κB pathways are reported to be associated with the production of mucin. , …”

Section: Discussionmentioning

confidence: 99%

Wogonoside Ameliorates Airway Inflammation and Mucus Hypersecretion via NF-κB/STAT6 Signaling in Ovalbumin-Induced Murine Acute Asthma

Yu,

Cai,

et al. 2024

J. Agric. Food Chem.

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“…Anti-inflammatory effects of KRG are associated with cytokine regulation and phagocytosis in innate immunity as well as the activation of B and T lymphocytes [ [44] , [45] , [46] ]. Ginsenosides such as ginsenoside-Rh2 (Rh2) and ginsenoside-Rg3 (Rg3) and their metabolites protopanaxatriol (PPT) and protopanaxadiol (PPD) have antioxidant, antitumor, anti-inflammatory and immunomodulatory activities [ [47] , [48] , [49] , [50] , [51] ]. For instance, Rg3 can significantly reduce the activity of NF-kB, elevate caspase-3 expression, and inhibit VEGF expression [ [52] , [53] , [54] , [55] , [56] ].…”

Section: Ginseng and Endometriosismentioning

confidence: 99%

Protective role of ginseng in endometriosis during covid-19

Song

Lee

2023

Journal of Ginseng Research

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“…To induce airway inflammation among various agents, including cigarette smoke extract, fly ash, lipopolysaccharides, and carbon black, diesel exhaust particles (DPM) have been reported to induce mucin and inflammation in NCI-H292 airway epithelial cells [20]. Based on this considerable evidence, it was recently reported that the airway inflammation of saponin was confirmed using an in vitro model in which DPM was treated with NCI-H292 [21].…”

Section: Introductionmentioning

confidence: 99%

Cell-Free Supernatant from Lactobacillus and Streptococcus Strains Modulate Mucus Production via Nf-κB/CREB Pathway in Diesel Particle Matter-Stimulated NCI-H292 Airway Epithelial Cells

Lee¹,

Kang²

2022

Molecules

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Airway epithelial cells are a major site of airway inflammation and may play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Diesel particulate matter (DPM) is associated with mucus hypersecretion and airway inflammation and has been reported to overexpress airway mucin in the NCI-H292 airway epithelial cells. Therefore, regulation of mucin hypersecretion is essential for developing novel anti-inflammatory agents. This study aimed to investigate the effects of cell-free supernatant (CFS) from Lactobacillus and Streptococcus on nitro oxide (NO) production in RAW264.7 and proteins associated with mucus production in NCI-H292 cells. We observed that NO production was reduced by CFS from Lactobacillus and Streptococcus in RAW 264.7, and MUC4, MUC5AC, and MUC5B gene expression was increased by phosphorylation of nuclear factor kappa B (NF-κB) p65 and cAMP response element-binding protein (CREB) in DPM-stimulated NCI-H292 cells. However, CFS from L. paracasei MG4272, MG4577, L. gasseri MG4247, and S. thermophilus MG5140 inhibited mRNA expression related to mucus production by downregulating the CREB/NfκB signaling pathway. These results suggest that CFS from L. paracasei MG4272, MG4577, L. gasseri MG4247, and S. thermophilus MG5140 can contribute as a strategic candidate to the prevention of airway inflammatory diseases caused by DPM.

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Saponin attenuates diesel exhaust particle (DEP)-induced MUC5AC expression and pro-inflammatory cytokine upregulation via TLR4/TRIF/NF-κB signaling pathway in airway epithelium and ovalbumin (OVA)-sensitized mice

Cited by 6 publications

References 26 publications

Wogonoside Ameliorates Airway Inflammation and Mucus Hypersecretion via NF-κB/STAT6 Signaling in Ovalbumin-Induced Murine Acute Asthma

Wogonoside Ameliorates Airway Inflammation and Mucus Hypersecretion via NF-κB/STAT6 Signaling in Ovalbumin-Induced Murine Acute Asthma

Protective role of ginseng in endometriosis during covid-19

Cell-Free Supernatant from Lactobacillus and Streptococcus Strains Modulate Mucus Production via Nf-κB/CREB Pathway in Diesel Particle Matter-Stimulated NCI-H292 Airway Epithelial Cells

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