Sanitary Assurance at Biosolids Composting Facilities: Assessing the Efficiency of Temperature-Contact Time Criterion

Isobaev, Pulat; Wichuk, Kristine; McCartney, Daryl; Neumann, Norman F.

doi:10.1080/1065657x.2019.1641446

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…Bacterial dormancy is a major factor restricting the effectiveness of harmless treatment of livestock waste. 39,40 In the present study, we demonstrated that the prevalent heat stress of 55 °C in livestock waste treatment can remain ESBLproducing pathogens survival through dormancy, which may be one of the reasons why composting and other related processes cannot completely eliminate them from livestock waste. Composting systems can be subject to wide temperature variations due to environmental factors.…”

Section: ■ Materials and Methodsmentioning

confidence: 76%

Neglected Drivers of Antibiotic Resistance: Survival of Extended-Spectrum β-Lactamase-Producing Pathogenic Escherichia coli from Livestock Waste through Dormancy and Release of Transformable Extracellular Antibiotic Resistance Genes under Heat Treatment

Wang

Lin

et al. 2023

Environ. Sci. Technol.

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Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae has caused a global pandemic with high prevalence in livestock and poultry, which could disseminate into the environment and humans. To curb this risk, heat-based harmless treatment of livestock waste was carried out. However, some risks of the bacterial persistence have not been thoroughly assessed. This study demonstrated that antibiotic-resistant bacteria (ARB) could survive at 55 °C through dormancy, and simultaneously transformable extracellular antibiotic resistance genes (eARGs) would be released. The ESBL-producing pathogenic Escherichia coli CM1 from chicken manure could enter a dormant state at 55 °C and reactivate at 37 °C. Dormant CM1 had stronger β-lactam resistance, which was associated with high expression of β-lactamase genes and low expression of outer membrane porin genes. Resuscitated CM1 maintained its virulence expression and multidrug resistance and even had stronger cephalosporin resistance, which might be due to the ultra-low expression of the porin genes. Besides, heat at 55 °C promoted the release of eARGs, some of which possessed a certain nuclease stability and heat persistence, and even maintained their transformability to an Acinetobacter baylyi strain. Therefore, dormant multidrug-resistant pathogens from livestock waste will still pose a direct health risk to humans, while the resuscitation of dormant ARB and the transformation of released eARGs will jointly promote the proliferation of ARGs and the spread of antibiotic resistance.

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Section: ■ Materials and Methodsmentioning

confidence: 76%

Neglected Drivers of Antibiotic Resistance: Survival of Extended-Spectrum β-Lactamase-Producing Pathogenic Escherichia coli from Livestock Waste through Dormancy and Release of Transformable Extracellular Antibiotic Resistance Genes under Heat Treatment

Wang

Lin

et al. 2023

Environ. Sci. Technol.

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“…Así mismo, los lodos residuales pasan por un proceso de estabilización para que puedan ser reutilizados, aprovechando sus características, especialmente los nutrientes. Sin embargo, los procesos de estabilización y saneamiento más utilizados pueden producir la entrada a este estado, como ha sido publicado en procesos de digestión aerobia y anaerobia (Fu et al, 2015;Jiang et al, 2013;Viau y Pecia, 2009) y composteo (Isobaev et al, 2019).…”

Section: Bacterias Vbnc En Los Sectores Productivosunclassified

Bacterias viables, pero no cultivables: Cuando los patógenos están presentes, pero no son detectados por los análisis convencionales

Gómez¹,

Moleón²,

Viñas³

et al. 2023

LATAM

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Según la OMS, anualmente 600 millones de personas enferman y 420 000 mueren por el consumo de alimentos contaminados; de ellos, 125 000 son niños menores de 5 años. Una fracción de estas cifras, se puede relacionar con la habilidad bacteriana para sobrevivir ante ambientes estresantes, ingresando al estado viable pero no cultivable (VBNC, por sus siglas en inglés), en el que no son detectadas por métodos convencionales. Estos microorganismos afectan a los sectores productivos, provocando pérdidas económicas directas e indirectas, afectando a la salud de las personas expuestas. En este artículo se revisan los esfuerzos por obtener las implicaciones de la existencia de bacterias VBNC en distintas matrices y cómo esta presencia compromete su seguridad microbiológica.

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Sanitary Assurance at Biosolids Composting Facilities: Assessing the Efficiency of Temperature-Contact Time Criterion

Cited by 2 publications

References 28 publications

Neglected Drivers of Antibiotic Resistance: Survival of Extended-Spectrum β-Lactamase-Producing Pathogenic Escherichia coli from Livestock Waste through Dormancy and Release of Transformable Extracellular Antibiotic Resistance Genes under Heat Treatment

Neglected Drivers of Antibiotic Resistance: Survival of Extended-Spectrum β-Lactamase-Producing Pathogenic Escherichia coli from Livestock Waste through Dormancy and Release of Transformable Extracellular Antibiotic Resistance Genes under Heat Treatment

Bacterias viables, pero no cultivables: Cuando los patógenos están presentes, pero no son detectados por los análisis convencionales

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