Sanguinarine Induces Bimodal Cell Death in K562 but not in High Bcl-2-expressing JM1 Cells

Weerasinghe, Priya; Hallock, Sarathi; Tang, Shou‐Ching; Liepins, Andrejs

doi:10.1078/0344-0338-00150

Cited by 31 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sanguinarine-induced apoptosis has been detected in human epidermoid carcinoma (A431) [8] , human prostate cancer (LNCaP, DU-145, PC-3) [22,23] , and breast cancer (MCF-7) cell lines [7] , human endocervical (HeLa) [24] , human melanoma (M4Beu), human colon adenocarcinoma (DLD-1), lung non-small cell carcinoma (A549) [7] , human uveal melanoma (OCM-1) [25] , histiocytic lymphoma (U937), myeloid leukemia (ML-1a) [6] , human erythroleukemia (K562, JM1) cell lines [6,[26][27][28] , immortalized human keratinocytes (HaCaT) [26] and human primary fibroblasts [7,26] . In addition to this information, we found that sanguinarine did not cause ototoxic effect on cochlear cells, while cisplatin caused 20% necrosis on cochlear cells.…”

Section: Discussionmentioning

confidence: 99%

Apoptotic Effects of Sanguinarine on the Organ of Corti 1 Cells: Comparison with Cisplatin

Çeçen¹,

Erçetin²,

Kırkım³

et al. 2015

Int Adv Otol

View full text Add to dashboard Cite

OBJECTIVE:Sanguinarine is an alkaloid obtained from the root of Sanguinaria canadensis and other plants from the Papaveraceae family and is well known to possess a broad range of biological functions, such as antimicrobial, antifungal, anti-inflammatory, and antineoplastic activities. We aimed to specify the in vitro effect of sanguinarine on the House Ear Institute-Organ of Corti 1 (HEI-OC1) cells and to compare this effect with the ototoxic effect of cisplatin (CDDP). MATERIALS and METHODS:We performed cell proliferation assay for determining the in vitro effect of sanguinarine alone and compared it with the effect of cisplatin. Flow cytometry annexin-V apoptosis detection was performed. RESULTS:We found that sanguinarine and CDDP inhibited the cell growth in a dose-dependant manner in HEI-OC1 cells after 24 h of incubation. In sanguinarine-treated group, apoptosis was 6.6%, necrosis was 26.7%, and the cell viability was 66.7%. Further, in CDDP-treated group, apoptosis was 5.6%, necrosis was 45.4%, and the cell viability was 48.7%. According to the annexin-V apoptosis detection results, we found that sanguinarine caused 3.9% apoptosis and 1.3% necrosis, while CDDP caused 2.9% apoptosis and 20% necrosis on HEI-OC1 cells. CONCLUSION:Our findings suggested that lower doses of sanguinarine are promising antineoplastic agents, which did not indicate any toxic effect on HEI-OC1 cells. Application of these data to clinical practice requires further support by in vivo studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Apoptotic Effects of Sanguinarine on the Organ of Corti 1 Cells: Comparison with Cisplatin

Çeçen¹,

Erçetin²,

Kırkım³

et al. 2015

Int Adv Otol

View full text Add to dashboard Cite

show abstract

“…The antiproliferative and/or pro-apoptotic activities of sanguinarine have been demonstrated in cells derived from several human cancers including epidermal (Ahmad et al, 2000), keratinocytes (Adhami et al, 2003;Reagan-Shaw et al, 2006), prostate (Malikova et al, 2006b;Adhami et al, 2004;Huh et al, 2006), cervical (Ding et al, 2002), breast Debiton et al, 2003;Holy et al, 2006), leukemia (Han et al, 2008;Weerasinghe et al, 2001c;Weerasinghe et al, 2001b;Weerasinghe et al, 2001a), lymphoma (Hussain et al, 2007), melanoma (Burgeiro et al, 2013;Hammerova et al, 2011;Serafim et al, 2008), colon (Lee et al, 2012;Matkar et al, 2008), colorectal (Han et al, 2013a;Lee et al, 2012;Pica et al, 2012), gastric (Choi et al, 2009), pancreatic (Ahsan et al, 2007), lung (Jang et al, 2009), neuroendocrine (Larsson et al, 2010), osteosarcoma (Park et al, 2010), and in rat glioblastoma cells (Han et al, 2007), bladde (Han et al, 2013b). However, the impact of sanguinarine on neuroblastoma cells has not been shown.…”

Section: Discussionmentioning

confidence: 99%

Promoting Effects of Sanguinarine on Apoptotic Gene Expression in Human Neuroblastoma Cells

Çeçen¹,

Altun²,

Erçetin³

et al. 2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Neuroblastoma is the most common extracranial solid tumor in children. Approximately half of the affected patients are diagnosed with high-risk poor prognosis disease, and novel therapies are needed. Sanguinarine is a benzophenanthridine alkaloid which has anti-microbial, anti-oxidant and anti-inflammatory properties. The aim of this study is whether sanguinarine has in vitro apoptotic effects and which apoptotic genes might be affected in the human neuroblastoma cell lines SH-SY5Y (N-myc negative), Kelly (N-myc positive, ALK positive), and SK-N-BE(2). Cell viability was analysed with WST-1 and apoptotic cell death rates were determined using TUNEL. After RNA isolation and cDNA conversion, expression of 84 custom array genes of apoptosis was determined. Sanguinarine caused cell death in a dose dependent manner in all neuroblastoma cell lines except SK-N-BE(2) with rates of 18% in SH-SY5Y and 21% in Kelly human neuroblastoma cells. Cisplatin caused similar apoptotic cell death rates of 16% in SH-SY5Y and 23% in Kelly cells and sanguinarine-cisplatin combinations caused the same rates (18% and 20%). Sanguinarine treatment did not affect apoptototic gene expression but decreased levels of anti-apoptotic genes NOL3 and BCL2L2 in SH-SY5Y cells. Caspase and TNF related gene expression was affected by the sanguinarine-cisplatin combination in SH-SY5Y cells. The expression of regulation of apoptotic genes were increased with sanguinarine treatment in Kelly cells. From these results, we conclude that sanguinarine is a candidate agent against neuroblastoma.

show abstract

“…SA-mediated caspase-3 activation was also seen in a human erythroleukemia K 562 cell line in the apoptosis-inducing concentration (4 μM) (ref. 41 ).…”

Section: +mentioning

confidence: 99%

“…40 ), human erythroleukemia (K 562, JM1) (ref. [40][41][42] ) cell lines, immortalized human keratinocytes (HaCaT) (ref. 25 ) and human primary ).…”

Section: Effects On Programmed Cell Deathmentioning

confidence: 99%

Effects of Sanguinarine and Chelerythrine on the Cell Cycle and Apoptosis

Malíková

Zdařilová²,

Hlobílková³

2006

BIOMED PAP

View full text Add to dashboard Cite

Sanguinarine Induces Bimodal Cell Death in K562 but not in High Bcl-2-expressing JM1 Cells

Cited by 31 publications

References 28 publications

Apoptotic Effects of Sanguinarine on the Organ of Corti 1 Cells: Comparison with Cisplatin

Apoptotic Effects of Sanguinarine on the Organ of Corti 1 Cells: Comparison with Cisplatin

Promoting Effects of Sanguinarine on Apoptotic Gene Expression in Human Neuroblastoma Cells

Effects of Sanguinarine and Chelerythrine on the Cell Cycle and Apoptosis

Contact Info

Product

Resources

About