Sandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70): Application in diagnosis/prognosis of coronavirus disease 2019 (COVID-19)

A novel label-free electrochemical immunosensor for the detection of heat shock protein 70 of lung adenocarcinoma cell line following paclitaxel treatment using l-cysteine-functionalized Au@MnO2/MoO3 nanocomposites

“…The electrochemically active surfaces associated with the signal transducer were quantified using the Randles–Sevcik equation: I = 2.69 × 10 5 × A × D 1/2 × n 3/2 × ν 1/2 × C . 46,47 Here, n signifies the number of transferred electrons, A represents the ECSA, D signifies the diffusion coefficient of K 3 [Fe(CN) 6 ], and C corresponds to the concentration of K 3 [Fe(CN) 6 ] solution (5 mM). Thus, with the known values of n , D , and C , along with the slope of the I vs. ν 1/2 plot, the value of ECSA can be calculated.…”

Sandwich-Like Electrochemical Immunosensing of Squamous Cell Carcinoma Antigen Based on Gold Nanoparticles/Reduced Graphene and Toluidine Blue/UIO-66-NH2

“…large surface area, good stability and conductivity, endowing itself to be an ideal carrier to assemble PAb for the construction of the STEM assay. [24][25][26] Another key aspect for the STEM assay is to design effective signal amplifier. UIO-66-NH 2 , a porous hierarchically zirconium-based MOFs nanomaterial, has large specific areas, stable porous structure and high porosity, 27,28 which provides an ideal platform for accommodating a large number of electroactive molecules as probes; meanwhile, there are abundant of -NH 2 groups on the surface of UIO-66-NH 2 , making it easily to modify with antibody.…”