Sandwich Enzymoimmunoassay of Hepatitis B Surface Antigen (HB<sub>s</sub>Ag)

Adachi, Hidenari; Fukuda, Takashi; Funahashi, Shuji; Kurahori, Tomohiro; Ishikawa, Eiji

doi:10.1111/j.1423-0410.1978.tb02925.x

Cited by 21 publications

(4 citation statements)

References 13 publications

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“…Serum C3 (70-176 mg/dl) and C4 (16-45 mg/ dl) were measured by radial immunodiffusion (14). Hepatitis B surFace antigen (15) and surface and core antibody (16) determinations were performed on sera from all patients and on the cryoprecipitates from 2 patients.…”

Section: Methodsmentioning

confidence: 99%

Two types of inflammatory vascular disease in Sjögren's syndrome. Differential association with seroreactivity to rheumatoid factor and antibodies to Ro (SS‐A) and with hypocomplementemia

Molina

Provost

Alexander

1985

Arthritis & Rheumatism

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Two types of inflammatory vascular disease (IVD) occur in Sjogren's syndrome: neutrophilic IVD (NIVD) and mononuclear IVD (MIVD). In 45 patients with Sjogren's syndrome, we examined the 2 types of IVD with respect to serologic associations. NIVD, unlike MIVD, was significantly associated with seroreactivity reflected by hyperglobulinemia (P = 0.01), rheumatoid factor (P = 0.002), antinuclear antibodies (P = 0.02), and antibodies to Ro (SS-A) (P = 0.00006), and with hypocomplementemia (P = 0.03). The differential association of the 2 types of IVD with serologic reactivity and hypocomplementemia suggests that there may be basic differences in the immunopathogenesis of these 2 forms of IVD in Sjogren's syndrome.Inflammatory vascular disease (IVD) is increasingly being recognized as a complication of Sjogren's syndrome (SS) (1,2). Two distinct types of IVD have been observed (2): neutrophilic IVD (NIVD) and mononuclear IVD (MIVD). We previously reported a Publication #570 of the O'Neill Research Laboratories,

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Section: Methodsmentioning

confidence: 99%

Two types of inflammatory vascular disease in Sjögren's syndrome. Differential association with seroreactivity to rheumatoid factor and antibodies to Ro (SS‐A) and with hypocomplementemia

Molina

Provost

Alexander

1985

Arthritis & Rheumatism

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“…The results confirm the usefulness of the Dot-EIA for HBsAg detection in sera. The sensitivity of the technique was equal to the standard-EIA [4,5], whereas it offers some advantages over standard EIA. Antiserum absorption to the well surface must be performed with overnight incubation for the standard EIA.…”

Section: Discussionmentioning

confidence: 98%

A rapid microassay for detecting hepatitis B surface antigen by dot enzyme immunoassay

Matsumura¹

1988

FEMS Microbiology Letters

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“…Serum albumin was quantified utilizing the technique with enhanced specificity of bromocresol green colorimetric assay (Diamond Diagnostics Kit, Germany) [ 25 ]. Electrochemiluminescence immunoassay (ECLA) on COBAS immunoassay analyzer was employed for assessing HCV antibody (anti-HCV) [ 26 ], while HBsAg was detected using Sorin Biomedica Co. kit (Italy) [ 27 ]. AFP was measured by enzyme-linked immunosorbent assay (ELISA) (IMMULITE 1000 system by a kit provided by Siemens Medical Solutions Diagnostics, USA) [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Inverse relationship between the level of miRNA 148a-3p and both TGF-β1 and FIB-4 in hepatocellular carcinoma

Dawood

Saleh

Elbahr

et al. 2021

Biochemistry and Biophysics Reports

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Background and aim Hepatocellular carcinoma (HCC) is a major health burden globally. Dysregulation of miRNA 148a-3p is engaged in carcinogenesis. TGF-β is a profibrogenic cytokine. This study assesses the expression level of miRNA 148a-3p and its relationship with serum TGF-β1 and fibrosis index based on four factors (FIB-4) in Egyptian patients with HCV-associated HCC. Subjects and Methods: The study included 72 HCC patients with HCV, 48 HCV cirrhotic patients, and 47 healthy controls. Serum TGF-β1 was assessed by ELISA and the expression of miRNA 148a-3p was measured by RT-PCR. Results Patients with HCC had lower plasma miRNA 148a-3p, higher serum TGF-β1, and higher FIB-4 levels than patients with cirrhosis and controls. miRNA 148a-3p discriminated HCC either from control (AUC: 0.997, 95.83% sensitivity, 85.11% specificity) or from cirrhosis (AUC: 0.943, 91.67% sensitivity, 81.25% specificity). Moreover, it distinguished metastatic from nonmetastatic patients (AUC: 0.800, 88.89% sensitivity, 60.0% specificity). The decreased miRNA 148a-3p and the increased TGF-β1 levels were related to distant metastasis, multinodular lesions, advanced TNM stage, and BCLC score (C). A negative correlation between miRNA 148a-3p and each of FIB-4 and TGF-β1 was detected. The decreased miRNA 148a-3p was associated with poor overall survival and poor progression-free survival. Conclusion An inverse relationship between miRNA 148a-3p and both TGF-β1 and FIB-4 was observed, which could be involved in HCC pathogenesis. Moreover, this miRNA is a potential diagnostic and prognostic biomarker for HCC.

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Sandwich Enzymoimmunoassay of Hepatitis B Surface Antigen (HB_sAg)

Cited by 21 publications

References 13 publications

Two types of inflammatory vascular disease in Sjögren's syndrome. Differential association with seroreactivity to rheumatoid factor and antibodies to Ro (SS‐A) and with hypocomplementemia

Two types of inflammatory vascular disease in Sjögren's syndrome. Differential association with seroreactivity to rheumatoid factor and antibodies to Ro (SS‐A) and with hypocomplementemia

A rapid microassay for detecting hepatitis B surface antigen by dot enzyme immunoassay

Inverse relationship between the level of miRNA 148a-3p and both TGF-β1 and FIB-4 in hepatocellular carcinoma

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Sandwich Enzymoimmunoassay of Hepatitis B Surface Antigen (HBsAg)

Cited by 21 publications

References 13 publications

Two types of inflammatory vascular disease in Sjögren's syndrome. Differential association with seroreactivity to rheumatoid factor and antibodies to Ro (SS‐A) and with hypocomplementemia

Two types of inflammatory vascular disease in Sjögren's syndrome. Differential association with seroreactivity to rheumatoid factor and antibodies to Ro (SS‐A) and with hypocomplementemia

A rapid microassay for detecting hepatitis B surface antigen by dot enzyme immunoassay

Inverse relationship between the level of miRNA 148a-3p and both TGF-β1 and FIB-4 in hepatocellular carcinoma

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Product

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About

Sandwich Enzymoimmunoassay of Hepatitis B Surface Antigen (HB_sAg)